Isolation and identification of microorganisms that promote growth and inoculation with banana seedlings in vitro

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.12080

Keywords:

Banana; Microorganisms; In vitro cultivation.

Abstract

The inoculation of growth-promoting bacteria has already shown benefits in different cultures such as increased biomass production for the growth of roots and stems, greater resistance to water stress conditions and changes in their physiological properties. Plant growth promoting bacteria have stood out for their potential application in plant production, allowing the reduction of the use of fertilizers and pesticides, which is due to the ability to stimulate the growth of plants by biological means by nitrogen fixation and production of phytohormones , such as indole-3-acetic acid (IAA). The aim of this study was to verify the potential for promoting plant growth of 25 wild bacteria from the Agricultural Microbiology Culture Collection at the Federal University of Lavras (CCMA-UFLA) by assessing the capacity for biological nitrogen fixation and IAA production; select growth-promoting bacteria, in addition to evaluating the effect of 3 isolates on seedlings of Musa sp. grown in vitro. The experiment was conducted in a completely randomized design (CRD), containing 3 treatments, plus the control, with 30 explants per treatment. The bacterium CCMA0056 caused the death of all seedlings in vitro. Banana growth is reduced when inoculated with the bacteria Azospirillum brasilense and Enterobacter cloacae.

References

Andrade, F. M., de Assis Pereira, T., Souza, T. P., Guimarães, P. H. S., Martins, A. D., Schwan, R. F., Pasqual, M., & Dóri, J. (2019). Beneficial effects of inoculation of growth-promoting bacteria in strawberry. Microbiol. Res., 223, 120–128.

Baldani, V. D., Baldani, J. I., & Döbereiner, J. (2000). Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp. Biol. Fert. Soils, 30, 485–491.

Cassán, F., Vanderleyden, J. & Spaepen, S. (2013). Physiological and agronomical aspects of phytohormone production by model plant-growth-promoting rhizobacteria (PGPR) belonging to the genus Azospirillum. J. Plant Growth Regul, 33, 440–459.

Dias, A. C. F., Costa, F. E. C, Andreote, F. D., Lacava, P. T., Teixeira, M. A., Assumpção, L. C., Araújo, W. L., Azevedo, J. L., & Melo, I. S. 2009. Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion. World J. Microb. Biot. 25, 189–195.

Döbereiner, J., Baldani, V. L. D., & Baldani, J. I. (1995). Como isolar e identificar bactérias diazotróficas de plantas não-leguminosas. Brasília: Embrapa-SPI.

Jausoro, V., Llorente, B. E., & Apo´stolo, N. M. (2010) Structural diferences between hyperhydric and normal in vitro shoots of Handroanthus impetiginosus (Mart. ex DC) Mattos (Bignoniaceae). Plant Cell Tissue Organ, 101, 183–191.

Jha, C. K., Patel, B., & Saraf, M. (2012). Stimulation of the growth of Jatropha curcas by the plant growth promoting bacterium Enterobacter cancerogenus msa2. World J. Microb. Biot, 28: 891–899.

Kloepper, J. W., Reddy, M. S., Rodríguez-Kabana, R., Kenney, D. S., Kokalis Burelle, N., & Martinez-Ochoa. (2004). Application for rhizobacteria in transplant production and yield enhancement. Acta Hort (ISHS), 631: 219-229.

Kuss, A. V., Kuss, V. V., Lovato, T., & Flôres, M. L. (2007). Fixação de nitrogênio e produção de ácido indolacético in vitro por bactérias diazotróficas endofíticas. Pesquisa Agropecuária Brasileira, 42, 1459-1465.

Loaces, I., Ferrando, L., & Scavino, A. F. 2011. Dynamics, diversity and function of endophytic siderophore producing bacteria in rice. Microb Ecol. 61, 606–618.

Loeper, J., Loeper, J., & Fragny, M. (1978). The physiological role of the silicone and its antiatheromatous action, in: Biochemistry of silicone and related problems. AVI Press, 2810306.

Oliveira, H. Comportamento de cultivares de bananeira (Musa spp.) resistentes a doença no processo de micropropagação. 2010. 79 f. Dissertação (Mestrado em Agronomia) – Universidade Federal Rural da Amazônia. Belém.

Pedrinho, E. A. N., Júnior, R. F. G., Campanharo, J. C., Alves, L. M. C. & Lemos, E. G. M. (2010). Identificação e avaliação de rizobactérias isoladas de raízes de milho. Bragantia, 69: 905-911.

Peixoto Neto, P. A. S., Azevedo, J. L., & Caetano, L. C. (2004). Microrganismos endofíticos em plantas: status atual e perspectivas. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 3(4), 69-72.

Pereira, G. V. M. De., Magalhães, K. T., Lorenzetii, E. R., Souza, T. P., & Scwan, R. F. A (2012). Multiphasic Approach for the Identification of Endophytic Bacterial in Strawberry Fruit and their Potential for Plant Growth Promotion. Microbial Ecology, 63:(2), 405–417.

Pereira, M. M. A., Morais, L. C., Marques, E. A., Martins, A. D., Cavalcanti, V. P., Rodrigues, F. A., Gonçalves, W. M., Blank, A. F.,Pasqual & M., Dória, J.(2019). Humic Substances and Efficient Microrganisms: Elicitation of Medicinal Plantas – A review. Journal of Agricultural Science; 11(7), 1-13.

Puente, M. E., Li, C. Y., & Bashan, Y. (2009) Endophytic bacteria in cacti seeds can improve the development of cactus seedlings. Environmental and Experimental Botany, 66:402–408.

Silva Neto, S. P. da. (2001). Propagação por biotecnologia. In: Ruggieiro, C. (Coord.). Bananicultura: FUNEP,128-149.

Souza, R., Ambrosini, A., & Passaglia, L. M. P. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology, Ribeirão Preto, 38 (4), 401-19.

Szilagyi-Zecchin, V. J., Mógor, A. F., Ruaro, L., & Röder, C. (2015). Crescimento de mudas de tomateiro (Solanum lycopersicum) estimulado pela bactéria Bacillus amyloliquefaciens subsp. plantarum fzb42 em cultura orgânica. Rev. Ciênc. Agr, 38, 26-33.

Vettori, L., Russo, A., Felici,C., Fiaschi, G., Morini , S., & Toffanin, A. (2010). Improving micropropagation: effect of Azospirillum brasilense Sp245 on acclimatization of rootstocks of fruit tree. Journal of Plant Interactions, 5, 4.

Published

30/01/2021

How to Cite

PEREIRA, M. M. A.; ARAÚJO, R. C. .; MARTINS, A. D. .; RODRIGUES, F. A. .; PASQUAL, M.; DÓRIA, J. Isolation and identification of microorganisms that promote growth and inoculation with banana seedlings in vitro. Research, Society and Development, [S. l.], v. 10, n. 1, p. e54010112080, 2021. DOI: 10.33448/rsd-v10i1.12080. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/12080. Acesso em: 25 apr. 2024.

Issue

Section

Agrarian and Biological Sciences