Microorganisms and Antimicrobial Resistance in complex wounds

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12161

Keywords:

Wounds and Injuries; Microbiota; Wound Infection; Drug resistance microbial.

Abstract

Objective: To characterize the microbiota of complex wounds and the antimicrobial resistance of the microorganisms found. Methods: Cross-sectional, observational and dissertative study, carried out based on the results of microbiological cultures of patients undergoing outpatient follow-up to treat complex wounds. Study approved under CAAE opinion number 93044418.6.0000.5013. Results: 110 tests were analyzed, collected from vasculogenic, diabetic, pressure ulcer, surgical, oncological, acute, anthrax lesions, fournier syndrome and marjolin ulcer. 22 species of bacteria were identified, 18 Gram-negative and 4 Gram-positive, with predominance of Pseudomonas aeruginosas, Proteus mirabilis and Staphylococcus aureus. All the microorganisms identified showed resistance to at least one antimicrobial, the P. mirabilis species being resistant to a greater number of antibiotics. Conclusion: This study points out that the complex wound microbiota is predominantly composed of Gram-negative microorganisms, the species P. aeruginosa stands out. Antimicrobial resistance was detected in all microorganisms found.

References

Pires, B. M. F. B. (2018) Análise microbiológica e capacidade de formação de biofilme bacteriano em cepas de Pseudomonas aeruginosa e Staphylococcus aureus encontrados em úlceras venosas tratadas com plasma rico em plaquetas (Dissertação de Mestrado). Universidade Federal Fluminense, Niterói, RJ; 2018. https://app.uff.br/riuff/bitstream/1/11809/1/TESE_Bruna%20Maiara.pdf.

Tedeschi, S., Negosanti, L., Sgarzani, R., Trapani, F., Pignanelli, S., Battilana, M. et al. (2017) Superficial swab versus deep-tissue biopsy for the microbiological diagnosis of local infection in advanced-stage pressure ulcers of spinal-cord-injured patients: a prospective study. Clinical Microbiology and Infection. 23(12), 943-947. 10.1016/j.cmi.2017.04.015.

Haalboom. M, Blokhuis-Arkes. M. H. E., Beuk, R. J., Meerwaldt. R., Klont. R., Schijffelen. M. J., Bowler. P. B., Burnet. M., Sigl. E., & Van der Pale, J. A. M. (2019) Culture results from wound biopsy versus wound swab: does it matter for the assessment of wound infection? Clinical Microbiology and Infection. 25, 629.e7e629.e12. 10.1016/j.cmi.2018.08.012

Rodrigues, W. C. C., Pinheiro, L. B., Lima, A. T., Battisti, L., Mota, M. A. G., Costa, M. C. et al. (2017) Epidemiological and clinical profile of the burned patients under physiotherapeutic care in the State University of Goiás. Rev Brasileira de Queimaduras 16(2), 94-99. http://www.rbqueimaduras.com.br/details/369/pt-BR.

Santos, K. C. B., Ribeiro, G. S. C., Feitosa, A. H. C., Silva, B. R. S., & Cavalcante, T. B. (2018) Quality of life of hospitalized patients with chronic wounds. Rev. Eletr. Enf. 20 (49). 10.5216/ree.v20.54130.

Santos, W. B., Araujo, M. G. S., Silva, J. C., Bernado, T. H. L., Bastos, M. L. S., & Verissímo, R. C. S. S. (2016) Surgical wounds infective microbiota: national and international analysis of scientific production. Rev.SOBECC. 21(1), 46-51. 10.5327/Z1414-4425201600010007.

Campos, M. G. C. A., Sousa, A. T. O., Vasconcelos, J. M. B., Lucena, S. A. P., & Gomes, S. K. A. (2016) Feridas complexas e estomias: aspectos preventivos e manejo clínico. João Pessoa: Ideia.

Souza, G. A. A. D., Garcia, L. M., Rocha, S. S., & Maciel, A. P. F. (2016) Microbiological profile of skin and soft tissue infections in hospitalized patients from a university hospital. R Epidemiol Control Infec. 6(1), 33-36. 10.17058/reci.v6i1.5901.

Silva, V., Macoleta, A., Silva, A., Flores, D., Aparicio, T., Aburto, I. et al. (2018) Prevalence and susceptibility pattern of bacteria isolated from infected chronic wounds in adult patients. Rev Chil Infectol. 35(2),155-162. 10.4067/s0716-10182018000200155.

Borges, E. L., Amorim, I. P. G., & Carvalho, D. V. (2014) Características dos Pacientes com Úlcera Venosa Atendidos nas Unidades de Atenção Primária de Nova Lima, Minas Gerais. Estima. 12(1), 31-42. https://www.revistaestima.com.br/index.php/estima/article/view/88.

Oliveira, A. F., & Oliveira Filho, H. (2014) Microbiological species and antimicrobial resistance profile in patients with diabetic foot infections. J Vasc Bras.13(4):289-293. 10.1590/1677-5449.0015.

Chudobova, D., Cihalova, K., Guran, R., Dostalova, S., Smerkova, S., Vesely, F. et al. (2015) Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration. Braz J Infec Dis. 19(6), 604-613. 10.1016/j.bjid.2015.08.013.

Khosravi, A. D., Hovezaivi, H., Mohammadian, A., Farahani, A., & Jenabi, A. (2016) Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR. Acta Cir Bras. 31(3), 206-211. 10.1590/S0102-865020160030000009.

Oliveira, F. P. (2019) Análise microbiológica de feridas tratadas com fator de crescimento epidérmico: estudo de coorte (Tese de Mestrado). Niterói: Universidade Federal Fluminense; 2019. https://app.uff.br/riuff/bitstream/1/11799/1/Fernanda%20Pessanha%20de%20Oliveir a%20TESE.pdf.

Mao, T., Zhai, H., Duan, Z., & Yang H. Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016. Polish J Microb. 2019;68(2):225-32. 10.33073/pjm-2019-024.

Soares, R. S. (2019) O perfil bacteriológico e as variáveis relacionadas a ferida neoplásica no paciente em cuidado paliativo (Tese de Mestrado). Niterói: Universidade Federal Fluminense. https://app.uff.br/riuff/bitstream/1/9596/1/Raquel%20de%20Souza%20Soares.pdf.

Downloads

Published

06/02/2021

How to Cite

OLIVEIRA, F. T. de; LIMA, G. K. S.; SANTOS, I. M. R. dos; SILVA, D. P. da; BERNARDO, T. H. L.; BASTOS, M. L. de A.; SILVA, P. S. G. da; BARROS, D. de A. Microorganisms and Antimicrobial Resistance in complex wounds. Research, Society and Development, [S. l.], v. 10, n. 2, p. e10110212161, 2021. DOI: 10.33448/rsd-v10i2.12161. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/12161. Acesso em: 19 apr. 2024.

Issue

Vol. 10 No. 2

Section

Health Sciences

License

Copyright (c) 2021 Fabianny Torres de Oliveira; Gabriella Keren Silva Lima; Igor Michel Ramos dos Santos; Davi Porfirio da Silva; Thaís Honório Lins Bernardo; Maria Lysete de Assis Bastos; Paulo Sérgio Gomes da Silva; Denise de Almeida Barros

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.