Relationship between viability of thawed semen and pregnancy rate of Nelore cows subjected to fixed-time artificial insemination
Keywords:Bovine; CASA system; Flow cytometry; Pregnancy.
This research aimed to evaluate the relationship of membrane integrity analysis, mitochondrial cytochemical analysis, and sperm kinetics of bovine semen after thawing and the pregnancy rate in Nelore females submitted to fixed-time artificial insemination (FTAI). Thirty-seven conventional semen straws, from 16 Nelore bulls and 3 Aberdeen Angus bulls were used. The semen samples were evaluated in conventional microscopy for sperm motility, vigor, and morphology, through flow cytometry for membrane integrity and mitochondrial function, and the CASA system for evaluation of sperm kinetics. All females were subjected to the same FTAI protocol and were inseminated by the same technical team. The correlation between all variables was performed by Pearson's correlation. Cluster analysis was used to analyze the relationship between pregnancy rate and combinations of laboratory tests (P ≤ 0.05). Semen samples from Angus bulls (89.2 ± 5.5) presented superior VSL (P=0.004) to Nelore semen (78.6 ± 8.6), but this did not affect the pregnancy rate (Nelore: 51.0% versus Angus: 50.5%, P > 0.05). By flow cytometry, 44.3% membrane integrity and 47.3% mitochondrial integrity were identified. Five clusters were selected, one of which presented the best pregnancy rate, containing the most balanced rates between progressive velocity and mitochondrial activity (VSL 71.16 μm/s, MITO 49.69%, TxP 62.4%). Samples of thawed bovine semen with progressive velocity and balanced mitochondrial activity demonstrated better fertility to TAI program.
Aitken, R. John, Ryan, A. L., Baker, M. A., & McLaughlin, E. A. (2004). Redox activity associated with the maturation and capacitation of mammalian spermatozoa. Free Radical Biology and Medicine, 36(8), 994–1010. https://doi.org/10.1016/j.freeradbiomed.2004.01.017
Aitken, R. J., Paterson, M., Fisher, H., Buckingham, D. W., & Van Duin, M. (1995). Redox regulation of tyrosine phosphosryllation in human spermatozoa and its role in the controlo f human sperm function. Free Radic Biol Med, 36, 994–1010.
Amann, R. P., & Waberski, D. (2014). Computer-assisted sperm analysis (CASA): Capabilities and potential developments. Theriogenology, 81(1), 5-17.e3. https://doi.org/10.1016/j.theriogenology.2013.09.004
Arruda, R. L., Orro, I. R., Passos, T. S., Costa e Silva, E. V., & Zúccari, C. E. S. N. (2010). Técnicas para avaliação laboratorial da integridade estrutural e funcional do sêmen congelado de touros. Revista Brasileira de Reprodução Animal, 168–184.
ASBIA. (2015). Relatório Estatístico De Importação, Exportação E Comercialização De Sêmen.
Baruselli, P. S., Ferreira, R. M., Sá, Filho, M. F., & Bó, G. A. (2018). Review: Using artificial insemination v. natural service in beef herds. Animal, 12(s1), s45–s52. https://doi.org/10.1017/S175173111800054X
Baruselli, P, Sales, J. N., Sala, R., Vieira, L., & Sá Filho, M. (2012). History, evolution and perspect ives of timed artificial in semination programs in Brazil. Animal Reproduction, 9(3), 139–152.
Baruselli, P. S, Reis, E. L., Marques, M. O., Nasser, L. F., & Bó, G. A. (2004). The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Animal Reproduction Science, 82–83, 479–486. https://doi.org/10.1016/j.anireprosci.2004.04.025
Bergstein, T. G., Weiss, R. R., & Bicudo, S. D. (2014). Técnicas de análise de sêmen. 189–194.
Blom, E. (1983). Pathological condictions in the genital organs and in the semen of group for rejection of breeding bulls for import or export to and from Denmark, 1958-1982. Nordisk Veterinaer Medicin, 3, 105–130.
Bó, G. A., Huguenine, E., de la Mata, J. J., Núñez-Olivera, R., Baruselli, P. S., & Menchaca, A. (2018). Programs for fixed-time artificial insemination in South American beef cattle. Animal Reproduction, 15(Irrs), 952–962. https://doi.org/10.21451/1984-3143-AR2018-0025
CBRA, C. B. de R. A.-. (2013). Manual para exame andrológico e avaliação de sêmen animal.
de Lamirande, E., & Cagnon, C. (1993). Human sperm hyperactivation and capacitation as parts of an oxidative process. Free Radical Biology and Medicine, 14(2), 157–166. https://doi.org/10.1016/0891-5849(93)90006-G
Fontes, P. L. P., Oosthuizen, N., & Cliff Lamb, G. (2020). Reproductive management of beef cattle. Animal Agriculture, 57–73. https://doi.org/10.1016/b978-0-12-817052-6.00004-5
Halliwell, B., & Gutteridge, J. M. C. (1999). Free Radicals in Biology and Medicine. (3rd ed.).
Hancock, G. L. (1959). The morphologic characteristics of spermatozoa and fertility. Internacional Journal of Fertility, 4, 347–359.
Hirao, K. (1975). A multiple regression analysis on six measurements of bovine semen characteristics for fertility. Japanese Journal of Veterinary Research, 4, 204–208.
Kathiravan, P., Kalatharan, J., Karthikeya, G., Rengarajan, K., & Kadirvel, G. (2011). Objective Sperm Motion Analysis to Assess Dairy Bull Fertility Using Computer-Aided System - A Review. Reproduction in Domestic Animals, 46(1), 165–172. https://doi.org/10.1111/j.1439-0531.2010.01603.x
Koppers, A. J., De Iuliis, G. N., Finnie, J. M., McLaughlin, E. A., & Aitken, R. J. (2008). Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. Journal of Clinical Endocrinology and Metabolism, 93(8), 3199–3207. https://doi.org/10.1210/jc.2007-2616
Lamirande, E. de, Tsai, C., Harakat, A., & Gagnon, C. (1998). Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates. Journal of Andrology, 19(1995), 585–594.
Machado, R., Corrêa, R. F., Barbosa, R. T., & Bergamaschi, M. A. C. M. (2008). Escore da condição corporal e sua aplicação no manejo reprodutivo de ruminantes. Circular Técnica, 57, 1–16.
Marques, M. de O., Morotti, F., Lorenzetti, E., Bizarro-Silva, C., & Seneda, M. M. (2018). Intensified use of TAI and sexed semen on commercial farms. Animal Reproduction, 15(3), 197–203. https://doi.org/10.21451/1984-3143-AR2018-0070
Martinez-Pastor, F., Johannisson, A., Gil, J., Kaabi, M., Anel, L., Paz, P., & Rodriguez-Martinez, H. (2004). Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen. Animal Reproduction Science, 84(1–2), 121–133. https://doi.org/10.1016/j.anireprosci.2003.12.006
Meneghetti, M., Filho, O. G. S., Peres, R. F. G., Lamb, G. C., & Vasconcelos, J. L. M. (2009). Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: Basis for development of protocols. Theriogenology, 72(2), 179–189. https://doi.org/10.1016/j.theriogenology.2009.02.010
Negreiros, M. P. M., Seugling, G. H. F., Almeida, A. B. M., Hidalgo, M. M. T., Martins, M., Isabel, M., Blaschi, W., & Barreiros, T. R. R. (2020). Influence of nutritional and ovarian parameters on pregnancy rates of Nelore cows artificially inseminated at fixed time. Research, Society and Development, 9(9), 1–18. http://www.elsevier.com/locate/scp
O’Connell, M., McClure, N., & Lewis, S. (2002). The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reproduction, 17(3), 704–709. https://doi.org/10.1093/humrep/17.3.704
Oliveira, L. Z., Arruda, R. P. de, Andrade, A. F. C. de, Celeghini, E. C. C., Reeb, P. D., Martins, J. P. N., Santos, R. M. dos, Beletti, M. E., Peres, R. F. G., Monteiro, F. M., & Hossepian de Lima, V. F. M. (2013). Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program. Animal Reproduction Science, 137(3–4), 145–155. https://doi.org/10.1016/j.anireprosci.2013.01.010
Pinheiro, O. L., Barros, C. M., Figueiredo, R. A., Valle, E. R., Encarna, R. O., Padovani, C. R., & Ib, F. (1998). Estrous Behavior And The Estrus-To-Ovulation Interval In Nelore Cattle ( Bos Indicus ) With Natural Estrus Or Estrus Induced With. Theriogenology, 55(014), 667–681.
Rodríguez-Martínez, H. (2003). Laboratory Semen Assessment and Prediction of Fertility : still Utopia ?* Outcomes from Routine Laboratory Sperm. Reproduction of Domestic Animals, 38, 312–318.
Rui, B., Bicudo, L., & Arruda, R. (2015). Ferramentas para avaliação da funcionalidade da mitocôndria espermática. Revista Brasileira de Reprodução Animal, 39(2), 277–283.
Sellem, E., Broekhuijse, M. L. W. J., Chevrier, L., Camugli, S., Schmitt, E., Schibler, L., & Koenen, E. P. C. (2015). Use of combinations of in vitro quality assessments to predict fertility of bovine semen. Theriogenology, 84(9), 1447-1454.e5. https://doi.org/10.1016/j.theriogenology.2015.07.035
Sudano, M. J., Crespilho, A. M. I., Fernandes, C. B., Junior, A. M., Papa, F. O., Rodrigues, J., MacHado, R., & Landim-Alvarenga, F. D. C. (2011). Use of bayesian inference to correlate in vitro embryo production and in vivo fertility in Zebu Bulls. Veterinary Medicine International, 2011, 10–12. https://doi.org/10.4061/2011/436381
Tortorella, R. D., Modesto, M. R., Neves, J. P., & Ramos, A. F. (2016). Development of fixed-time artificial insemination protocols for locally adapted Curraleiro Pé-Duro cows. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 68(5), 1159–1167. https://doi.org/10.1590/1678-4162-8555
Troiano, L., Granata, A. R. M., Cossarizza, A., Kalashnikova, G., Bianchi, R., Pini, G., Tropea, F., Carani, C., & Franceschi, C. (1998). Mitochondrial membrane potential and DNA stainability in human sperm cells: A flow cytometry analysis with implications for male infertility. Experimental Cell Research, 241(2), 384–393. https://doi.org/10.1006/excr.1998.4064
Tsakmakidis, I. A. (2010). Ram semen evaluation: Development and efficiency of modern techniques. Small Ruminant Research, 92(1–3), 126–130. https://doi.org/10.1016/j.smallrumres.2010.04.017
Vianna, F. P., Papa, F. O., Zahn, F. S., Melo, C. M., & Dell’Aqua, J. A. (2009). Thermoresistance sperm tests are not predictive of potential fertility for cryopreserved bull semen. Animal Reproduction Science, 113(1–4), 279–282. https://doi.org/10.1016/j.anireprosci.2008.06.009
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Copyright (c) 2021 Fernando Roberto Pereira; Ana Beatriz Marques de Almeida; Myrian Megumy Tsunokawa Hidalgo; José Roberto Potiens; Anne Kemmer Souza; Flávio Guiselli Lopes; Thales Ricardo Rigo Barreiros; Fábio Morotti; Maria Isabel Mello Martins
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