Phytochemical, physicochemical, microbiological study and anticholinesterase activity of Ginkgo biloba L. and Bacopa monnieri L. used in phytotherapy

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13480

Keywords:

Quality control; Phytochemical analysis; Anticholinesterase activity; Ginkgo biloba L.; Bacopa monnieri L.

Abstract

Herbal medicines based on Ginkgo biloba L. and Bacopa monnieri L. are used to improve memory and cognitive function. The quality control and anticholinesterase activity of the herbal medicines prepared from G. biloba and B. monnieri were evaluated which are commercialized in handling pharmacies. Samples of herbal medicines based on G. biloba and B. monnieri were obtained from handling pharmacies in Teresina-PI, submitted to labeling analysis and anticholinesterase activity. The phytochemical study was performed by preliminary prospecting, TLC and HPLC. The tests physicochemical and microbiological analyses were made according to the Pharmacopoeia 2010. It was observed that the labeling, foreign material, disintegration, and microbiological parameters were in accordance with ANVISA standards. The colorimetric tests were uniform in the samples of B. monnieri and variable of G. biloba. The average weight evaluation shows that the G1 and B3 samples have capsular content above that described in the labeling and presented pH values ​​different. Among the thermogravimetric profiles, the mass losses of samples G1 and B3 showed values ​​with high ash content, suggesting adulterations. Phytochemical prospecting showed flavonoids as common secondary metabolites in both species, corroborating with TLC and HPLC analysis, which identified the compounds chlorogenic acid, rutin, myricetin and quercetin. The samples G4, B2 and B5 present metabolites capable of inhibiting the enzyme acetylcholinesterase with IC50 of 0.8540 mg/mL, 0.9650 mg/mL and 1.8221×10-5 mg/mL, respectively. The samples G1 and B3 of G. biloba and B. monnieri, did not obey some parameters of quality control for herbal medicines according to the criteria of the Brazilian Pharmacopeia.

Author Biographies

Tatiana de Oliveira Lopes, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Jaqueline Fernanda de Sousa Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Rudielson dos Santos Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renata da Silva Carneiro, Piaui State University

Departamento de Química, PPGQ -GERATEC- Universidade Estadual do Piauí, Teresina, PI, CEP-64002-150, Brasil.

Antônio Rafael de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Yáscara Lopes de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renato Pinto de Sousa, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil

Clara Andrezza Crisóstomo Bezerra Costa, Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

 

Iron Jonhson de Araújo Veras, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil

Ronaldo dos Santos Sousa Júnior, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Johnnatan Duarte de Freitas , Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

Mahendra Rai, SGB Amravati University

Department of Biotechnology, SGB Amravati University, Amravati, 444 602, Maharashtra State, India

Laécio Santos Cavalcante, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Francisca Lucia de Lima, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Mariana Helena Chaves, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Chistiane Mendes Feitosa, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Valdiléia Teixeira Uchôa, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

References

Andrade, F. R. O., Souza, A. A., Arantes, M. C. B., De Paula, J. R., & Bara, M. T. F. (2013) Microbiological analysis of raw materials and masterful pharmaceutical formulations. Revista Eletronica de Farmacia, 2(2), 38-44.

Azad, A. K., Awang, M., & Rahman, M. M. (2012) Phytochemical and microbiological evaluation of a local medicinal plant Bacopa monnieri (L.) Penn. Internacional Journal of Current Pharmaceutical Review and Resrarch, 3(3), 66-78.

Banov, D., Baby, A. R., Del Bosco, L. M., Kaneko, T. M., & Velasco, M. V. R. (2006) Characterization of dry extract of Ginkgo biloba L. in topical formulations. Acta Farmaceutica Bonaerense 25, 219-224.

Bonfilio, R., Santos, O. M. M., Novaes, Z. R., Matinatti, A. N. F., & De Araújo, M. B. (2013) Physical, chemical and microbiological quality control in 2347, handled in 2010 and 2011. Revista de Ciências Farmacêuticas Básica e Aplicada, 34(4), 527-535.

Brasil. Ministério da Saúde. 2010. Agência Nacional de Vigilância Sanitária, Resolução – RE nº 89, de 16 de março de 2003.Lista de Registro Simplificado de Fitoterápicos. Brasília.

Brasil. Ministério da Saúde. 2007. Agência Nacional de Vigilância Sanitária. Resolução RDC n° 67, de 08 de outubro de 2007. Dispõe sobre Boas Práticas de Manipulação de Preparações Magistrais e Oficinais para Uso Humano em farmácias. Diário Oficial da República Federativa do Brasil, Brasília, DF.

Brasil. Ministério da Saúde. 2004. Agência Nacional de Vigilância Sanitária, Resolução – RE nº 89, de 16 de março de 2004.Lista de Registro Simplificado de Fitoterápicos. Brasília.

Cavalcante, A. N., Feitosa, C. M., Santos, F. P. S., Sousa, A. P. R., Júnior, R. S.S., Souza, A. A., Pinto, B. F., Araújo, C. M., & Rashed, K. (2018) Elaboration and characterization of the inclusion complex between β-cyclodextrin and the anticholinesterase 2-oleyl-1,3-dipalmitoyl-glycerol extracted from the seeds of MART. Journal of Molecular Structure, 1177, 209-216. https://doi.org/10.1016/j.molstruc.2018.09.067

Chakraborty, P. (2018) Herbal genomics as tools for dissecting new metabolic pathways of unexplored medicinal plants and drug discovery. Biochimie Open, 6, 9–16. https://doi.org/10.1016/j.biopen.2017.12.003

Chaves, M. H. (1997) Analysis of plant extracts by ccd: a methodology applied to the discipline “Organic Chemistry”. Química Nova, 20(5), 560-562. https://doi.org/10.1590/S0100-40421997000500019.

Dai, C. X., Hu, C. C., Shang, Y. S., & Xie, J. (2018) Role of Ginkgo biloba extract as an adjunctive treatment of elderly patients with depression and on the expression of serum S100B. Medicine 97(39), e12421. https://doi.org/10.1097/md.0000000000012421.

Dudley, E., Ptang, J., Newton, R. P., & Brenton, A. G. (2008) Fingerprint Profile Of Ginkgo Biloba Nutritional Supplements By LC/ESI-MS/MS. PHYTOCHEMISTRY, 69(7), 1555-1564. HTTPS://DOI.ORG/10.1016/J.PHYTOCHEM.2008.01.026

Dutta, S., & Chakraborty, S. (2020) Estimation of phytochemical characteristics and antioxidant property of secondary metabolites of a memory enhancing medicinal herb Baccopa monnieri of East Kolkata Wetland. Internacional Journal of Pharmaceutical Science and Resrarch 11(6), 2860-2867. http://dx.doi.org/10.13040/IJPSR.0975-8232.11(6).2860-67

Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 88-90. https://doi.org/10.1016/0006-2952(61)90145-9

Esteves, B., Marques, A. V., Domingos, I., & Pereira, H. (2008) Heat-induced colour changes of pine (Pinus pinaster) and eucalyptus (Eucalyptus globulus) wood. Wood Science and Technology, 42, 369-384. https://doi.org/10.1007/s00226-007-0157-2

Farmacopeia Brasileira. 2012. F.N. (2a ed.), Brasília, DF.

Fazlul, M. K. K., Deepthi, S. P., Mohammed, I., Farzana, Y., Munira, B., & Nazmul, M. H. M. (2019) Antibacterial and antifungal activity of various extracts of Bacopa monnieri. Journal of Pharmaceutical Research Internacional, 11(1), 1698-1702. https://doi.org/10.3923/ijp.2008.230.232.

Gayoso, L. C., Moreno, I., Puerta, E., Suárez-Santiago, J. E., Santos-Magalhães, N. S., Ramirez, M. J., & Irache, J. M. (2017) Effect of the oral administration of nanoencapsulated quercetin on a mouse model of Alzheimer‘s disease. International Journal of Pharmaceutics, 517(1-2), 50-57. https://doi.org/10.1016/ j.ijpharm.2016.11.061

Guo, Y., Wang, T., Fu, F-F., El-Kassaby, Y. A., & Wang, G. (2020) Temporospatial flavonoids metabolism variation in Ginkgo biloba leaves. Frontiers in Genetics, 11, 589326. https://doi.org/10.3389/fgene.2020.589326

Ibrahim, M. P., & Nuhu, A. A. (2016) Phytochemical screening and antibacterial/antifungal activities of Ginkgo biloba extract EGB 761. Journal of Pharmacy and Biological Science, 11(1), 43-49. https://doi.org/10.9790 / 3008-11124349.

Jadiya, P., Khan, A., Sammi, S. R., Kaur, S., & Nazir, A. (2011) Anti-Parkinsonian effects of Bacopa monnieri: Insights from transgenic and pharmacological Caenorha-biditis elegans models of Parkinson´s disease. Biochemical and Biophysical Research Communications, 413(4), 605-610. https://doi.org/10.1016/ j.bbrc.2011.09.010

Kim, M. S., Bang, J. H., Lee, J., Han, J. S., Baik, T. G., & Jeon, W. K. (2016) Ginkgo biloba L. Extract protects against chronic cerebral hypoperfusion by modulating neuroinflammation and the cholinergic system. Phytomedicine, 23(12), 1356–1364. https://doi.org/10.1016 /j.phymed.2016.07.013

Kongkeaw, C., Dilokthornsakul, P., Thanarangsarit, P., Limpeanchob, N., & Scholfield, N. (2014) Meta-analysis of randomized controlled trials on cognitive effects of Bacopa monnieri extract. Journal of Ethnopharmacology, 151(1), 528-535. https://doi.org/10.1016/j.jep.2013.11.008.

Le, X. T., Pham, H. T., Do, P. T., Fujiwara, H., Tanaka, K., Li, F., Nguyen, T. V., Nguyen, K. M., & Matsumoto, K. (2013) Bacopa monnieri ameliorates memory deficits in olfactory bulbectomized mice: possible involvement of glutamatergic and cholinergic systems. Neurochemical Research, 38, 2201-2215. https://doi.org/10.1007/s11064-013-1129-6.

Lu, J., Zheng, Y. L., Luo, L., Wu, D. M., Sun, D. X., & Feng, Y. J. (2006) Quercetin reverses D-galactose induced neurotoxicity in mouse brain. Behavioural Brain Research, 171(2), 251-260. https://doi.org/10.1016/j.bbr.2006.03.043

Macêdo, S. K. S., Lima, K. S. B., Rolim-Neto, P. J., Rolim, L. A., & Nunes, X. P. (2020) Pharmacognostic, thermal and morphological characterization of the bark and leaves of Triplaris gardneriana Wedd. Brazilian Journal of Development, 6(3), 11904-11915. https://doi.org/10.34117/bjdv6n3-158

McLaren, K. (2008) The Development of the CIE 1976 (L*a*b*) uniform colour-space and colour-difference formula. Coloration Technology, 92, 338-341. https://doi.org/10.1111/j.1478-4408.1976.tb03301.x

Mathew, J., Paul, J., Nandhu, M. S., & Paulose, C. S. (2010) Bacopa monnieri and Bacoside-A for ameliorating epilepsy associated behavioral deficits. Fitoterapia 81(5), 315-322. https://doi.org/10.1016/j.fitote.2009.11.005

Matos FJA. (2009). Introduction to experimental phytochemistry. Fortaleza: Edições UFC.

Müller WE, Eckert A, Eckert GP, Fink H, Friedland K, Gauthier S, Hoerr R, Ihl R, Kasper S, Möller H (2019) Therapeutic efficacy of the Ginkgo special extract EGb761® within the framework of the mitochondrial cascade hypothesis of Alzheimer’s disease. The World Journal of Biological Psychiatry, 20(3), 1814-1412. https://doi.org/ 10.1080/15622975.2017.1308552

Oliveira ACD, Ropke CD (2016) The ten years of the National Policy on Medicinal Plants and Herbal Medicines (PNPMF) and the main obstacles in the production chain of plant extracts and herbal medicines in Brazil. Revists Fitos, 10(2), 185-198. https://doi.org/10.5935/2446-4775.20160015

Park SY (2010) Potential therapeutic agents against Alzheimerʼs disease from natural sources. Archives of Pharmacal Research, 33, 1589-1609. https://doi.org/10.1007/ s12272-010-1010-y.

Pincelli ALPSM, De Moura LF, Brito JO (2012) Effect of thermal rectification on colors of Eucalyptus saligna and Pinus caribaea woods. Maderas Ciencia y Tecnologia 14(2), 239-248. http://dx.doi.org/10.4067/S0718-221X2012000200010

Radha P, Sumathi S, Padma PR (2011) Antioxidant status of oxidant challenged rats treated with Bacopa monnieri leaf extract. Journal of Pharmaceutical Research, 4(10), 3538-3539.

Ramasamy S, Chin SP, Sukumaran SD, Buckle MJC, Kiew LV, Chung LY (2015) In Silico e In Vitro analysis of Bacoside A aglycones e seus derivados como os constituintes responsáveis pelos efeitos cognitivos de Bacopa monnieri. PLoS ONE 10(5), e0126565. https://doi.org/10.1371/journal.pone.0126565

Rao S, Rajkumar P, Kaviraj C, Parveen, PA (2012) Efficient plant regeneration from leaf explants of Bacopa monniera (L.) Wettst.: A threatened medicinal herb. Annals of Phytomedicine, 1(1), 110-117.

Rhee IK, De Meent MV, Ingkaninan K, Verpoorte R (2001) Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. Journal of Chromatography A 915(1-2), 217-223. https://doi.org/10.1016/S0021-9673(01)00624-0

Russo A, Borrelli F (2005) Bacopa monnieri, a Reputed Nootropic Plant: An Overview. Phytomedicine 12(4), 305-318. https://doi.org/10.1016/j.phymed.2003.12.008

Saini N, Singh D, Sandhir R (2012) Neuroprotective effects of Bacopa monnieri in experimental model of dementia. Neurochemical Research, 37, 1928-1937. https://doi.org/10.1007/s11064-012-0811-4

Sherma J, Rabel F (2020) Review of advances in planar chromatography-mass spectrometry published in the period 2015–2019, Journal of Liquid Chromatography and Related Technologies, 43(11-12), 394-412. https://doi.org/10.1080/10826076.2020.1725561

Silva LO, Silva RL (2014) Quality control regarding the determination of weight in capsules handled in pharmacies in the city of Mogi Guaçu, Foco, 7, 41-60.

Singh B, Kaur P, Singh G, Ahuja P (2008) Biology and chemistry of Ginkgo biloba. Fitoterapia, 79(6), 401-418. https://doi.org/10.1016/j.fitote.2008.05.007

Soobrattee MA, Neergheen VS, Luximon-Ramma A (2005) Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research, 579(1-2), 200-213. https://doi.org/10.1016/ j.mrfmmm.2005.03.023

Song J, Fang G, Zhang Y, Deng Q, Wang S (2010) Fingerprint analysis of Ginkgo biloba leaves and related health foods by highperformance liquid chromatography/electrospray ionization-mass spectrometry. Journal of AOAC International, 93(6), 1798–1805. https://doi.org/10.1093/jaoac/93.6.1798

Tian J, Liu Y, Chen K (2017) Ginkgo biloba extract in vascular protection: molecular mechanisms and clinical applications. Current Vascular Pharmacolohy, 15, 532-548. https://doi.org/10.2174/1570161115666170713095545.

Tosun I, Ustun N, Tekguler B (2008) Physical changes and post-ripening of blackberry fruits. Scientia Agrícola, 65(1), 87-90. https://doi.org/10.1590/S0103-90162008000100012.

Wang D, Zhao J, Li S, Shen G, Hu S (2018) Quercetin attenuates domoic acid-induced cognitive deficits in mice. Nutritional Neuroscience, 21(2), 123-131. https://doi.org/10.1080/1028415X.2016.1231438

Vinutha B, Prashanth D, Salma K, Sreeja SL, Pratiti D, Padmaja R, Radhika S, Amit U, Venkateshwarlu K, Deepak H (2007) Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. Journal of Ethnopharmacology, 109(2), 359-63. https://doi.org/10.1016/j.jep.2006.06.014

Yamchuen P, Chaiwiang N, Lapphanichayakool P, Ingkaninan K, Limpeanchob N (2017) Neuroprotective effect of Bacopa monnieri extract on oxidized low density lipoprotein-induced neurotoxicity in SH-SY5Y neuroblastoma cells. Thai Journal of Pharmacology, 39(1), 5-18.

Yang G, Wang Y, Sun J, Zhang K, Liu J (2016) Commetary: Ginkgo biloba for mild cognitive impairment and Alzheimer's disease: A systematic review and meta-analysis of randomized controlled trials. Journal of Neurologia and Neuromedicine, 1(8), 4-6. https://doi.org/10.2174/1568026615666150813143520.

Youdim KA, Dobbie MS, Kuhnle G, Proteggente AR, Abbott NJ, Rice-Evans C (2003) Interaction between flavonoids and the blood-brain barrier: in vitro studies. Journal of Neurochemistry, 85(1), 180-192. https://doi.org/10.1046 / j.1471-4159.2003.01652.x

Zhanga L, Li D, Cao F, Xiao W, Zhao L, Ding G, Wang ZZ (2018) Identification of human acetylcholinesterase inhibitors from the constituents of EGb761 by modeling docking and molecular dynamics simulations. Combinatorial Chemistry & High Throughput Screening, 21(1), 41-49. https://doi.org/10.2174/1386207320666171123201910

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20/03/2021

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LOPES, T. de O. .; SILVA, J. F. de S. .; SILVA, R. dos S. .; CARNEIRO, R. da S. .; OLIVEIRA, A. R. de .; OLIVEIRA, Y. L. de .; SOUSA, R. P. de .; COSTA, C. A. C. B. .; VERAS, I. J. de A. .; SOUSA JÚNIOR, R. dos S. .; FREITAS , J. D. de .; RAI, M.; CAVALCANTE, L. S. .; LIMA, F. L. de .; CHAVES, M. H. .; FEITOSA, C. M. .; UCHÔA, V. T. . Phytochemical, physicochemical, microbiological study and anticholinesterase activity of Ginkgo biloba L. and Bacopa monnieri L. used in phytotherapy. Research, Society and Development, [S. l.], v. 10, n. 3, p. e39010313480, 2021. DOI: 10.33448/rsd-v10i3.13480. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/13480. Acesso em: 24 apr. 2024.

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Vol. 10 No. 3

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Health Sciences

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Copyright (c) 2021 Tatiana de Oliveira Lopes; Jaqueline Fernanda de Sousa Silva; Rudielson dos Santos Silva; Renata da Silva Carneiro; Antônio Rafael de Oliveira; Yáscara Lopes de Oliveira; Renato Pinto de Sousa; Clara Andrezza Crisóstomo Bezerra Costa; Iron Jonhson de Araújo Veras; Ronaldo dos Santos Sousa Júnior; Johnnatan Duarte de Freitas ; Mahendra Rai; Laécio Santos Cavalcante; Francisca Lucia de Lima; Mariana Helena Chaves; Chistiane Mendes Feitosa; Valdiléia Teixeira Uchôa

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