In vitro evaluation of the acaricidal activity of propolis against cattle ticks
DOI:
https://doi.org/10.33448/rsd-v10i7.16203Keywords:
Tick control; Rhipicephalus (Boophilus) microplus; Natural substance.Abstract
Propolis is a natural substance that is produced by bees and has been used since ancient times for its pharmacological properties. The cattle tick Rhipicephalus (Boophilus) microplusis responsible for great economic losses by cattle breeding programs. The aim of the present study was to evaluate the in vitro effect of propolis extracts on engorged female ticks. The experiment was performed at the Uberaba IFTM. A completely randomized experimental design was used, including eight treatments and five replicates, with a total 40 experimental units, each consisting of 10 engorged females of homogeneous weight. The experimental units were immersed in alcoholic propolis extracts that were diluted with distilled water to the following concentrations: 0, 10, 20, 30, 40, 50, 60, and 70%. A regression analysis was used to analyze the relationship between propolis extract concentration and weight of eggs laid (WEL), hatching percentage (HP), and reproductive efficiency (RE). This demonstrates the viability of propolis as an alternative for the control of cattle ticks, with the 70% extract concentration being most efficientand most effective for controlling R. microplus under laboratory conditions.
References
Andreotti, R., Garcia, M.V & Koller, W.W. (2019). Carrapatos na Cadeia Produtiva de Bovinos. Empresa Brasileira de Pesquisa Agropecuária Embrapa Gado de Corte. Ministério da Agricultura, Pecuária e Abastecimento, 240.
Bispo, J. L. P., Almeida, E. C. de.; Nunes, D. M. (2020).Efeito do extrato bruto de jenipapo (Genipa americana) no controle do carrapato bovino Rhipicephalus (Boophilus) microplus. Research, Society and Development. 9 (9), e481997308. 10.33448/rsd-v9i9.7308. https://rsdjournal.org/index.php/rsd/article/view/7308.
Barreta, A. A., Arruda, C., Miguel, F. G., Baptista, N., Nascimento, A. P., Oliveira, F.M., Hori, J. I., Barud, H. S., Damaso, B., Ramos, C., Ferreira, R & Kenupp, J. (2017). Functional Properties of Brazilian Propolis: From Chemical Composition Until the Market. Superfood and Functional Food - An Overview of Their Processing and Utilization.57-98. doi.org/10.5772/110001.
Bergeron, M.G & Ouellette, M. (1998). Preventing Antibiotic Resistance through Rapid Genotypic Identification of Bacteria and of Their Antibiotic Resistance Genes in the Clinical Microbiology Laboratory. Journal of Clinical Microbiology.Washington, v. 36, (8), 2169-2172.
Branco, L. A., Oliveira, H. L. M. de, Bortolucci, W. de C., Fernandez, C. M. M., Gonçalves, J. E., Gazim, Z. C., Gonçalves, D. D., Lourenço, E. L. B., Borges, J. L., Silva, V. dos S., Piau Junior, R. (2020). Controle de carrapato bovino (Rhipicephalus microplus) com óleo essencial de folhas de Psidium rufum DC. Research, Society and Development. 9, (11), e409119550. 10.33448/rsd-v9i11.9550.
Brasil. Ministério da Agricultura. Portaria nos 90 de 4 de dezembro de 1989. Normas para produção e utilização de produtos antiparasitários. Diário Oficial [da] República Federativa do Brasil, Brasília, 22 jan. seção 1, coluna. 2, 1990. Acessado em 10 de janeiro 2021.
Castaldo, S. & Capasso, F. (2002). Propolis, an old remedy used in modem medicine. Fitoterapia, 73, S1-S6.
Castro, M. L., Cury, J. A R. P. L., Alencar, S. M., Ikegaki, M., Duarte, S & Koo, H. (2007). Própolis do sudeste e nordeste do Brasil: influência da sazonalidade na atividade antibacteriana e composição fenólica. Química Nova, 30 (7), 1512-1516. https://doi.org/10.1590/S0100-40422007000700003.
Cazella, L. N., Oliveira, H. L. de M., Bortolucci, W. de C., Rahal, I. L., Baretta, I. P., Gonçalves, J. E., Piau Junior, R., Fernandez, C. M. M., Linde, G. A., Colauto, N. B., Gazim, Z. C. (2020). Óleo essencial das folhas e flores de Baccharis dracunculifolia DC (Asteraceae) para controle de Rhipicephalus microplus Canestrini (Arachnida: Ixodidae) na fase de vida livre. Research, Society and Development. v. 9 (10), e5049108788, 2020. 10.33448/rsd-v9i10.8788.
Ghisalberti, E. L. (1979) Propolis: a review. Bee world, 60: 59-84. 10.1080/0005772X.1979.11097738.
Gonçalves, V. M., Huerta, M. M & Freitag, A. A. (2016). Acaricide plant potential in the control of Rhipicephalus (Boophilus) microplus. Revista de Ciência Veterinária e Saúde Pública, 3 (1) 014-022.
Heinzen, E. L., Cosendey, E. T. M. P., Gazzoni, J. G., Conceição Garcia, R. T. E. O., Oliveira, N. T. E & de Oliveira Orsi, R. (2012). Extrato de própolis no controle de helmintoses em bezerros. ActaVeterinaria Brasileira, 6 (1), 40-44.
Marcucci, M. C. (1996). Propriedades biológicas e terapêuticas dos constituintes químicos da própolis. Química Nova, 19 (5), 529-535.
Mazzuco, H., Silva, R. D. De M. E., Berchieri, J. R, A & Oliveira, E. De. (1996). Utilização da própolis e álcool etílico no controle de salmonella em rações avícolas. Scientia Agricola, 53(1). https://doi.org/10.1590/S0103-90161996000100001.
Mello, B. C. B. S., Petrus, J. C. C & Hubinger, M. D. (2010). Desempenho do processo de concentração de extratos de própolis por nanofiltração. Ciência e Tecnologia de Alimentos, 30 (1), 166-172. https://doi.org/10.1590/S0101-20612010000100025.
Mendes, T. M., Balbino, J. N. F., Torres Silva, N. C & de Farias, L. A. (2019). Rhipicephalus (Boophilus) microplus e Rhipicephalussanguineus: uma revisão sobre as perspectivas, distribuição e resistência. Pubvet. 13 (6), 127.
Pacheco, K. M. G. (2001). Própolis em diferentes concentrações utilizadas no controle do Boophilus microplus, In: ZOOTEC. Anais da Sociedade Brasileira de Zootecnia. Recife.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM.https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Principal, J., Hernández, I., D’Aubeterre, R & Rodrigues, J. G. (2002). Eficacia del propóleo em elcontrol de lashelmintiasis de ovinos naturalmente infestados. Revista Científica de la Universidaddel Zulia, Maracaibo12 (2), 604-607.
Santana, L. F., Carvalho Junior, R. A., Moreira, E. F. A., Rodrigues, E. A., Filho, J. M. P., Freitas, B.B B & Oliveira, C. B. (2019). Regional study of action in vitro of commercial acaricides on the cattletick. Revista Inova Ciência & Tecnologia, 5 (1), 51-55.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Alyne Madja dos Santos Silva; Édimo Fernando Alves Moreira; Ana Paula Espindula; Lorena Ferreira Benfica; Rosemar Alves de Carvalho Júnior; Luis Fernando Santana
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
