Investigation of gastrintestinal parasites in rabbits of the species Oryctolagus cuniculus in the Northwest region of São Paulo, Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.19130

Keywords:

Coccidiosis; Eimeria spp.; Endoparasites; Protozoan.

Abstract

Enteroparasites can be pathogenic for the rabbit population and for this reason, we have investigated its occurrence in bioterium of rabbits. The sample group was composed of 103 adult males of the species Oryctolagus cuniculus and New Zealand breed. Fecal samples (n = 103) were processed using the Willis Techniques (Fluctuation in saturated sodium chloride solution with specific density of 1.20 g/mL) and Faust (Centrifugal-flotation in zinc sulphate with density of 1.18 g/ml). The diameters of larger and smaller sizes of the sporocysts and sporozoites of the coccidia were measured by means of optical microscopy using the program CellSens 1.17_00_en_Rhine_13Feb2017. The Faust Technique showed positivity of 39.74% (31/78) for Eimeria spp, while in Willis Technique, 35.90% (28/78) was observed for Eimeria spp, being the species Eimeria perforans and in 51.28% (47/78) of them there was no parasitism. We identified, for the first time, the occurrence of the specie E. perforans in rabbits of the species O. cuniculus, New Zealand breed kept in a bioterium. Therefore, it is extremely important for the implantation of parasitic control of rabbits kept in bioterium for scientific purposes, the monitoring of possible infections by means of periodic coproparasitological exams.

References

Abdalla, M. A. E. & Seker, H. (2017). Recognition of Protozoan Parasites from Microscopic Images: Eimeria species in Chickens and Rabbits as a case study. Institute of Electrical and Eletronics Engineers.

Abdalla, M. A. E., Seker H. & Jiang, R. (2015). “Automated identification of chicken Eimeria species from microscopic images,” 2015 IEEE 15th Int. Conf. Bioinforma. Bioeng. 2, 1–4.

Abdalla, M. A. E., Seker, H. & Jiang, R (2016). “Identification of Rabbit Coccidia by Using Microscopic Images”, ICEMIS. 3–6.

Bachene, M. S., Temim, S., Ainbaziz, H. & Bachene, A (2019). Prevalence of Rabbit Coccidia in Medea Province, Algeria. World Vet. J. 9, (2), 123-128.

Castañón, C. A. B., Fraga, J. S., Fernandez, S., Gruber, A. & Costa, L. F. (2007). “Biological shape characterization for automatic image recognition and diagnosis of protozoan parasites of the genus Eimeria,” Pattern Recognit. 40, 7, 1899–1910.

Chroustova, E. & Pinka, K. (1987). The efficiency of disinfectants on the oocysts of Eimeria tenella, Acta vet.brno. 56, 141-149.

Conour, L. A., Murray, K. A. & Brown, M. J. (2006). Preparation of Animals for Research—Issues to Consider for Rodents and Rabbits. ILAR Journal, 47, 4.

Coudert, P., Licois, D. & Drouet-Viard, F. (1995). Eimeria species and strains of rabbits, In Biotechnology Guidelines on techniques in coccidiosis research. Eckert, J., Braun, R., Shirley, M. W., Coudert, P., Sc, Eds. Luxembourg: European commission. 52-73.

Daugschies, A., Bangoura, B. & Lendner, M. (2013). Inativação de estágios de endoparasita exógenos por desinfetantes químicos: estado atual e perspectivas. Parasitol Res. 112, 917–932. 10.1007/s00436-013-3324-4.

Eckert, J., Taylor, M., Licois, D., Coudert, P., Catchpole, J. & Bucklar, H. (1995). Identification of Eimeria and Isospora species and strains. Morphological and biological characteristics. In: Eckert J, Braun, R, Shirley, MW, Coudert, P (Ed.). Biotechnology. Guidelines on Techniques in Coccidiosis Research. Office for official publications of the European communities. Luxembourg. 103-119.

Elshahawi, G. A., El-Fayomi, H. M. & Abdel-Haleem, H. M. (2012). Coccidiosis of domestic rabbit (Oryctolagus cuniculus) in Egypt: light microscopic study. Parasitol Res. 110:251–258.

Elshahawi, І. & Elgoniemy, А. (2018). An Epidemiolog-ical Study on Endoparasites of Domestic Rabbits (Oryctolagus cuniculus) in Egypt with Special Refer-ence to Their Health Impact. sains Malaysiana. 47, 1, 9–18.

Faust, E. C., Sawitz, W., Tobie, J., Odom, V., Peres, C. & Lincicome, D. R. (1939). Comparative Efficiency of Various Technics for the Diagnosis of Protozoa and Helminths in Feces. J Parasitol. 25, 3, 241-62.

Gu, X., Liu, H., Li, C., Fang, S., Cui, P., Liao, Q., Zhang, S., Wang, S., Duan, C., Yu, F., Suo, X. & Liu, X. (2019). Selection and characterization of a precocious line of Eimeria media. Parasitology Research.

Guide for the care and use of laboratory animals (2011). Institute for Laboratory Animal Research. The national academies press 500 Fifth Street, NW Washington, Eighth Edition, DC 20001.

Harcourt-Brown, F (2014). Digestive system diseases. In: Meredith, A., Lord, B. (Eds.),BSAVA Manual of Rabbit Medicine. British Small Animal Veterinary Association, 168–190.

Inácio, S. V., Gomes, J. F., Falcão, A. X., Suzuki, C. T. N., Nagata, W. B., Loiola, S. H. N., Santos, B. M., Soares, F. A., Rosa, S. L., Baptista, C. B., Alves, G. B. & Bresciani, K. D. S. (2020). Automated Diagnosis of Canine Gastrointestinal Parasites Using Image Analysis. Pathogens. 9, 139, 10.3390/pathogens9020139.

Jing, F., Yin, G. W., Liu, X. Y., Suo, X. & Qin, Y. H. (2012). Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China. Parasitology Research. 110. (4), 1495-1500. 10.1007/s00436-011-2653-4.

Jing, J., Liu, C., Zhu, S. X., Jiang, Y. M., Wu, L. C., Song, H. Y. & Shao, Y. X. (2016). Pathological and ultrastructural observations and liver function analysis of Eimeria stiedai-infected rabbits. Vet Parasitol.

Krause, J., Mcdonnell, G. & Riedesel, H. (2001). Biodecontamination of animal rooms and heatsensitive equipment with vaporized hydrogen peroxide. Contemp Top Lab Anim Sci. 40:8-21.

Mäkitaipale, J., Karvinen, I., Virtala, A. K. & Näreaho, A (2017). Prevalence of intestinal parasites and risk factor analysis for Eimeria infections in Finnish pet rabbits. Veterinary Parasitology: Regional Studies and Reports.

Maziz-Bettahar, S., Aissi, M., Ainbaziz, H., Bachene, M.S., Zenia, S. & Ghisani, F. (2018). Prevalence of coccidian infection in rabbit farms in North Algeria. Veterinary World. 11, (11), 1569-1573.

Ming-Hsien, L., Hai-I, H. & Hong-Kean, O. (2010). Prevalence, infectivity and oocyst sporulation time of rabbit-coccidia in Taiwan. Trop Biomed. 27, 424–429.

Okumu, P. O., Gathumbi, P., Karanja, D. N., Mande, J. D., Wanyoike, M. M., Gachuiri, C. K., Kiarie, N., Mwanza R. N. & Borter, R. N. (2014). Prevalence, pathology and risk factors for coccidiosis in domestic rabbits (Oryctolagus cuniculus) in selected regions in Kenya, Veterinary Quarterly. 34, (4), 205-210.

Ola-Fadunsin, S. D., Nuhu, A. A., Fabiyi, J. P., Sanda, I. M., Hussain, K., Rabiu, M. & Ganiyu, I. A. (2019). Prevalence and associated risk factors of Eimeria species in rabbits (Oryctolagus cuniculus) in Ilorin, Kwara State, Nigeria. Annals of Parasitology. 65 (3), 267-273.

Pakandl, M. (2009). Coccidia of rabbit: A review. Folia Parasitol. 56, 153–166. 10.14411/fp.2009.019.

Papeschi, C., Fichi, G. & Perrucci, S. (2013). Oocyst excretion pattern of three intestinal Eimeria species in female rabbits. World Rabbit Science. 21, (2), 77-83. 10.4995/wrs.2013.1235.

Pereira, M. G. (2001). Epidemiology theory and practice. Guanabara Koogan. 596.

Ruddy, M. & Kibbler, C. C. (2002). Endoscopic decontamination: an audit and practical review. J Hosp Infect. 50, 261-268.

Schlolaut, W., Hudson, R. & Rödel, H. G. (2013). Impact of rearing management on health in domestic rabbits: A review. World Rabbit Science. 21, (3), 145-159. 10.4995/wrs.2013.1029.

Shek, W. R. & Gaertner, D. J. (2002). Controle de qualidade microbiológica para roedores de laboratório e lagomorfos, 365-393. In: Fox, J. G., Anderson, L. C., Loew, F. M., & Quimby, F. W. Medicina animal de laboratório. Academic Press.

Silva, A. S., Ceolin, L. & Monteiro, S. G. (2006). Rabbit endoparasitoses reared in different management systems. Jornal FZVA, Uruguaiana. 13, 2, 127-136.

Singla, L. D., Juyal, P. D. & Andhu, B. S. (2000). Patologia e terapia em coelhos naturalmente infectados por Eimeria stiedae. J. Protozool. Res. 10, 185 – 191.

Suzuki, C. T. N., Gomes, J. F., Falcao, A. X., Papa, J. P. & Hoshino-Shimizu, S. (2013). “Automatic segmentation and classification of human intestinal parasites from microscopy images,” IEEE Trans. Biomed. Eng. 60, 3, 803–812.

Willis, H. H. A (1921). Simple Levitation Method for the Detection of Hookworm Ova. Med J Aust <https://www.cabdirect.org/cabdirect/abstract/19222900461>.

Downloads

Published

15/08/2021

How to Cite

SILVA, D. R. R. da .; INÁCIO, S. V.; NAGATA, W. B. .; BAPTISTA, C. B. .; BASSI, A. P. F. .; GOMES, J. F. .; NAKAMURA, A. A. .; BRESCIANI , K. D. S. . Investigation of gastrintestinal parasites in rabbits of the species Oryctolagus cuniculus in the Northwest region of São Paulo, Brazil. Research, Society and Development, [S. l.], v. 10, n. 10, p. e468101019130, 2021. DOI: 10.33448/rsd-v10i10.19130. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/19130. Acesso em: 24 apr. 2024.

Issue

Section

Health Sciences