Comparative study between Monascus species for the production of natural pigments using agroindustrial waste as a substrate
DOI:
https://doi.org/10.33448/rsd-v10i11.19558Keywords:
Antioxidant activity; Cassava bran; Monascus pigments.Abstract
The filamentous fungus species Monascus produce natural pigments of different colors, in addition to bioactive compounds with antioxidant potential, being able to metabolize low cost substrates. However, there are several species of this genus that can exhibit different behaviors in relation to the production of pigments in the same production medium. Thus, the work proposal was to evaluate the behavior of two species of the genus Monascus, M. ruber URM 4530 and M. purpureus CCT 3802 regarding pigment production and, from the best results, evaluate the antioxidant activity of capturing ABTS+• radicals and DPPH• using cassava bran as a fermentative substrate and monosodium glutamate as a nitrogen source, with the aid of a Central Composite Design (DCC). As a result, better production and antioxidant activity were verified when using the M. purpureus strain, presenting 13.77 UA510 for the production of pigments and 96.1 % and 62.7 % inhibition of the radicals ABTS+• and DPPH•, respectively. In contrast, the M. ruber strain had a maximum production of 5.82 UA510. Thus, the results indicated different performances of the species of the genus Monascus, using the same residue as substrate and process conditions, with better pigment production performance and antioxidant activity for the strain M. purpureus CCT 3802.
References
Almeida, A. B., Santos, N. H., de Lima, T. M., Santana, R. V., de Oliveira Filho, J. G., Peres, D. S., & Egea, M. B. (2021). Pigment bioproduction by Monascus purpureus using corn bran, a byproduct of the corn industry. Biocatalysis and Agricultural Biotechnology, 32. https://doi.org/10.1016/j.bcab.2021.101931
Aniya, Y., Ohtani, I. I., Higa, T., Miyagi, C., Gibo, H., Shimabukuro, M., & Taira, J. (2000). Dimerumic acid as an antioxidant of the mold, Monascus anka. Free Radical Biology and Medicine, 28(6), 999-1004. 10.1016/s0891-5849(00)00188-x
Bei, Q., Liu, Y., Wang, L., Chen, G., & Wu, Z. (2017). Improving free, conjugated, and bound phenolic fractions in fermented oats (Avena sativa L.) with Monascus anka and their antioxidant activity. Journal of Functional Foods, 32, 185-194. https://doi.org/10.1016/j.jff.2017.02.028
Cheng, J., Choi, B. K., Yang, S. H., & Suh, J. W. (2016). Effect of fermentation on the antioxidant activity of rice bran by Monascus pilosus KCCM 60084. Journal of Applied Biological Chemistry, 59(1), 57-62. https://doi.org/10.3839/jabc.2016.011
Cruz, E. A., Melo, M. C., Santana, N. B., Franco, M., de Santana, R. S. M., Santos, L. S., & Gonçalves, Z. S. (2015). Produção de alfa-Amilase por Aspergillus niger em resíduo de cascas de mandioca. Journal of Health Sciences, 13(4). https://doi.org/10.17921/2447-8938.2011v13n4p%25p
Embaby, A. M., Hussein, M. N., & Hussein, A. (2018). Monascus orange and red pigments production by Monascus purpureus ATCC 16436 through co-solid state fermentation of corn ob and glycerol: An eco-friendly environmental low cost approach. PLoS One, 13(12). https://doi.org/10.1371/journal.pone.0207755
Jin, Y. J., & Pyo, Y. H. (2017). Effect of monascus-fermented soybean extracts on antioxidant and skin aging-related enzymes inhibitory activities. Preventive Nutrition and Food Science, 22(4), 376. 10.3746/pnf.2017.22.4.376
Kantifedaki, A., Kachrimanidou, V., Mallouchos, A., Papanikolaou, S., & Koutinas, A. A. (2018). Orange processing waste valorisation for the production of bio-based pigments using the fungal strains Monascus purpureus and Penicillium purpurogenum. Journal of Cleaner Production, 185, 882-890. https://doi.org/10.1016/j.jclepro.2018.03.032
Lermen, F. H., de Souza Matias, G., Modesto, F. A., Röder, R., & Boikor, T. J. P. (2015). Teste de consumidores e análise de aparência, sabores e cores para o desenvolvimento de novos produtos: O case do Projeto de Broinhas de Milho Saboreadas. Revista Latino-Americana de Inovação e Engenharia de Produção, 3(4), 97-110. http://dx.doi.org/10.5380/relainep.v3i4.37744
Liu, J., Luo, Y., Guo, T., Tang, C., Chai, X., Zhao, W., & Lin, Q. (2020). Cost-effective pigment production by Monascus purpureus using rice straw hydrolysate as substrate in submerged fermentation. Journal of Bioscience and Bioengineering, 129(2), 229-236. https://doi.org/10.1016/j.jbiosc.2019.08.007
Lv, J., Zhang, B. B., Liu, X. D., Zhang, C., Chen, L., Xu, G. R., & Cheung, P. C. K. (2017). Enhanced production of natural yellow pigments from Monascus purpureus by liquid culture: the relation ship between fermentation conditions and mycelial morphology. Journal of Bioscience and Bioengineering, 124(4), 452-458. https://doi.org/10.1016/j.jbiosc.2017.05.010
Martinez, D. G., & Feiden, A. (2017). Potencial do resíduo do processamento da mandioca para produção de etanol de segunda geração. Revista Brasileira de Energias Renováveis, 6(2), 141-148. 10.5380/rber.v6i2.45811
Moussa, S., Abdou, D., Mohamed, G. A., Abo-El-Seoud, M. A., Karam Eldin, A. Z. A., & El-mehalawy, A. A. (2018). Production of red pigment by Monascus purpureus Nrrl 1992 under submerged and solid-state fermentation. Egyptian Journal of Microbiology, 53(1), 83-94. 10.21608/EJM.2018.3962.1059
Nnolim, N. E., Okoh, A. I., & Nwodo, U. U. (2020). Proteolytic bacteria isolated from agro-waste dumpsites produced keratinolytic enzymes. Biotechnology Reports, 27, e00483. https://doi.org/10.1016/j.btre.2020.e00483
Panesar, R., Kaur, S., & Panesar, P. S. (2015). Production of microbial pigments utilizing agro-industrial waste: a review. Current Opinion in Food Science, 1, 70-76. https://doi.org/10.1016/j.cofs.2014.12.002
Rocha, D. S., & Reed, E. (2014). Pigmentos Naturais em Alimentos e sua Importância para a Saúde. Revista EVS-Revista de Ciências Ambientais e Saúde, 41(1), 76-85. http://revistas.pucgoias.edu.br/index.php/estudos/article/viewFile/3366/1953
Sánchez-Muñoz, S., Mariano-Silva, G., Leite, M. O., Mura, F. B., Verma, M. L., da Silva, S. S., & Chandel, A. K. (2020). Production of fungal and bacterial pigments and their applications. In Biotechnological Production of Bioactive Compounds, 327-361. https://doi.org/10.1016/B978-0-444-64323-0.00011-4
Seon, H. Y., Sun, S., & Yim, S. H. (2020). Correlation of the free radical and antioxidant activities of Eriobotrya Japonica Lindl. with phenolic and flavonoid contents. Food Science and Technology. http://orcid.org/0000-0002-0227-5484
Shi, K., Song, D., Chen, G., Pistolozzi, M., Wu, Z., & Quan, L. (2015). Controlling composition and color characteristics of Monascus pigments by pH and nitrogen sources in submerged fermentation. Journal of Bioscience and Bioengineering, 120(2), 145-154. https://doi.org/10.1016/j.jbiosc.2015.01.001
Silbir, S., & Goksungur, Y. (2019). Natural red pigment production by Monascus purpureus in submerged fermentation systems using a food industry waste: Brewer’s spent grain. Foods, 8(5), 161. https://doi.org/10.3390/foods8050161
Silva, J. R., Da Silva, T. T., Da Silva, É. K., Da Silva, S. P., Moreira, K. A., & Ribeiro, D. S. (2018). Produção de pigmentos de Monascus ruber CCT 3802 utilizando casca de mandioca como substrato. Revista Brasileira de Agrotecnologia, 8(3), 26-31.https://www.gvaa.com.br/revista/in dex.php/REBAGRO/article/view/6321
Srianta, I., Zubaidah, E., Estiasih, T., Iuchi, Y., Harijono, H., & Yamada, M. (2017). Antioxidant activity of pigments derived from Monascus purpureus fermented rice, corn, and sorghum. International Food Research Journal, 24(3), 1186-1191. http://repository.wima.ac.id/id/eprint/16353
Tseng, Y. H., Yang, J. H., Chang, H. L., Lee, Y. L., & Mau, J. L. (2006). Antioxidant properties of methanolic extracts from monascal adlay. Food Chemistry, 97(3), 375-381. https://doi.org/10.1016/j.foodchem.2005.04.022
Vendruscolo, F., Schmidell, W., de Oliveira, D., & Ninow, J. L. (2017). Kinetic of orange pigment production from Monascus ruber on submerged fermentation. Bioprocess and Biosystems Engineering, 40(1), 115-121. 10.1007/s00449-016-1679-5
Wang, L., Dai, Y., Chen, W., Shao, Y., & Chen, F. (2016). Effects of light intensity and color on the biomass, extracellular red pigment, and citrinin production of Monascus ruber. Journal of Agricultural and Food Chemistry, 64(50), 9506-9514. 10.1021/acs.jafc.6b04056
Woiciechowski, A. L., Carvalho, J. C., Spier, M. R., & Soccol, C. (2015). Emprego de resíduos agroindustriais em bioprocessos alimentares. Biotecnologia de Alimentos, 1, 143-171. 10.13140/RG.2.1.1508.7529
Yuliana, A., Singgih, M., Julianti, E., & Blanc, P. J. (2017). Derivates of azaphilone Monascus pigments. Biocatalysis and Agricultural Biotechnology, 9, 183-194. https://doi.org/10.1016/j.bcab.2016.12.014
Zhang, B. B., Xing, H. B., Jiang, B. J., Chen, L., Xu, G. R., Jiang, Y., & Zhang, D. Y. (2018). Using millet as substrate for efficient production of monacolin K by solid-state fermentation of Monascus ruber. Journal of Bioscience and Bioengineering, 125(3), 333-338. https://doi.org/10.1016/j.jbiosc.2017.10.011
Zhang, X. W., Wang, J. H., Chen, M. H., & Wang, C. L. (2013). Effect of nitrogen sources on production and photostability of Monascus pigments in liquid fermentation. IERI Procedia, 5, 344-350. https://doi.org/10.1016/j.ieri.2013.11.114
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Copyright (c) 2021 Thayná Torres da Silva; José Renato da Silva; Alana Emilia Soares de França Queiroz; Daniele Silva Ribeiro
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