Effects of sealants on in vitro demineralization of enamel surrounding orthodontic brackets

Authors

DOI:

https://doi.org/10.33448/rsd-v10i11.19923

Keywords:

Orthodontics; Dental Caries; Diagnostic; Sealants.

Abstract

The in vitro study considered the effects of CLINPRO™ XT and FLUROSHIELD® sealants in preventing demineralization in dental enamel in the region circumscribed to orthodontic brackets, through microhardness and scanning electron microscopy. Sixty crowns of bovine teeth were used, randomly divided into groups(n: 20): G1 (brackets/control), G2 (brackets/CLINPRO™) and G3 (brackets/FLUROSHIELD®). The samples were submitted to cariogenic challenge (DES/RE), reviewed for Knoop microhardness (25 g, 10 s), on the surface of the dental enamel, from the bracket at distances of 1,000 µm, 2,000 µm and 3,000 µm and 100 µm, 200 µm, 300 µm and 400 µm at the in-depth interface, in the incisal and cervical directions. SEM images were obtained from the surface in the cervical region. The results analyzed by ANOVA and Tukey statistical tests (p<0.05) showed significant influence on the microhardness values in the Treatment/Distance and Treatment/Distance/Region interactions (p=0.00) in groups G2 and G3 showed greater microhardness of enamel in areas closest to the bracket when sealant is applied, higher microhardness values with CLINPRO™ sealant. In samples that did not receive sealant, no influence of these interactions with microhardness was observed (p>0.05). CLINPRO™ sealant showed the highest microhardness and effectiveness values when compared to the control and FLUROSHIELD sealant groups. It was concluded that sealants were more effective in preventing enamel demineralization in regions closer to the brackets and less effective in more distant regions. The chemical composition and properties of the sealant interfere with its action to reduce demineralization of tooth enamel.

References

Albuquerque, A. C. L., Araújo, O. M. C., Silva, D. F. & Pereira, A. R. B. (2014). Avaliação clínica do custo/benefício de selantes oclusais. Rev Saúde Ciência on line, 3(1), 69-80.

AL-Jobair, A. (2010). In vitro Evaluation of Microleakage in Contaminated Fissures Sealed with GC Fuji Triage Glass Ionomer Cement. J King Saud UnivDental Sci, 22(1), 25-32.

Ananda, S.R. & Mythri, H. (2014). A comparative study of fluoride release from two different sealants. J Clin Exp Dent, 1;6(5): e497-501.

Araujo, A. A., Braca, L. S., Dietrich, L., Caixeta, D. A. F., Santos-Filho, P. C. F., & Martins, V. M. (2020). Caries detection and diagnosis methods: a narrative review. Res Soc Dev, 9 (11).

Artun J. & Brobakken B. O. (1986). Prevalence of carious white spots after orthodontic treatment with multibonded appliances. Eur J Orthod, 8, 229-234.

Behnan, S. M., Arruda. A. O., González-Cabezas, C., Sohn, W. & Peters, M. C. (2010). In-vitro evaluation of various treatments to prevent demineralization next to orthodontic brackets. Am J Orthod Dentofacial Orthop, 138(1): 712-717.

Benkaddour, A., Bahije, L., Bahoum, A. & Zaoui, F. (2014). Orthodontics and enamel demineralization: clinical study of risk factors. Int Orthod, 12, 458-466.

Bonifacio, C. C., Navarro, R.S., Sardenberg, F., Imparato, J. C. P., Carvalho, R. C. R. & Raggio, D. P. (2009). Microleakage of an adhesive system used as a fissure sealant. J Contemp Dent Pract, 2, 26-33.

Buren, J. L., Stley, R. N., Wefel, J. & Qian, F. (2008). Inibition of enamel demineralization by an enamel sealant Pro Seal: An in vitro study. M J Orthod Dentofacial Orthop, 133, 88-94.

Canali, G. D., Rached, R. N., Mazur, R. F. & Souza, E.M. (2017). Effect of Erosion/Abrasion Challenge on the Dentin Tubule Occlusion Using Different Desensitizing Agents. Braz Dent J, 28(2), 216-224.

Chapman, J. A., Robert, W. E., Eckert, G. J., Kula, K. S. & González-Cabezas, C. (2010). Risk factors for incidence and severity of white spot lesions during treatment with fixed orthodontic appliances. Am J Orthod Dentofacial Orthop, 138, 188-194.

Coordes, S. L., Jost-Brinkmann, P. G., Präger, T. M., Bartzela, T., Visel, D., Jäcker, T. & Müller-Hartwich R. (2018). A comparison of different sealants preventing demineralization around brackets. Comparative Study J Orofac Orthop, 79(1), 49-56.

Cruz, I. C., Neto, M. M. G., Lima, W. T. S., Silva, W. A., & Hora, S. L. (2020). New diagnostic methods for detecting dental caries - Integrative review. Res Soc Dev, 9(10).

Demito, C. F., Vivaldi-rodrigues, G., Ramos, A. L. & Bowman, S. J. (2004). The efficacy of a fluoride varnish in reducing enamel demineralization adjacent to orthodontic brackets: an in vitro study. Orthod Craniofacial Res, 7, 205–210.

Farhadian, N., Miresmaeili, A., Eslami, B. & Mehrabid, S. (2008). Effect of fluoride varnish on enamel demineralization around brackets: An in-vivo study. Am J Orthod Dentofacial Orthop, 133, 95-8.

Gondim, R. C. D., Lima, D. M., Costa, J. F. & Bauer, J. R. O. (2011). Dentinal hypersensitivity in noncarious cervicallesions: therapeutic approaches in pain control. Rev Pesq Saúde, 12(1), 52-33.

Gorelick, L., Geiger, A. M. & Gwinnett, A. J. (1982). Incidence of white spot formation after bonding and banding. Am J Orthod, 81, 93-98.

Gorton, J. & Featherstone, J. D. (2003). In vivo inhibition of demineralization around orthodontic brackets. Am J Orthod Dentofacial Orthop, 123(1), 10-14.

Hadler-Olsen, S., Sandvik, K., El-Agroudi, M. A. & Ogaard, B. (2012). The incidence of caries and white spot lesions in orthodontically treated adolescents with a comprehensive caries prophylactic regimen—a prospective study. Eur J Orthod, 34, 633-639.

Haghighi, A. H. S., Mohamadi, M. Z. B. & Nezhad, M. E. (2011). Evaluation of effect of different adhesives on demineralization around the metal brackets. Int J Clin Pediatr Dent, 26(4), 2-4.

Hajrassiea, M. K. A & Khier, S. E. (2007). In-vivo and in-vitro comparison of bond strengths of orthodontic brackets bonded to enamel and debonded at various times. Am J Orthod Dentofacial Orthop, 131(1), 384-390.

Hesse, D., Bonifácio, C. C., Mendes, F.M., Braga, M. M., Imparato, J. C. & Raggio, D. P. (2014). Sealing versus partial caries removal in primary molars: a randomized clinical trial. BMC Oral Health, 14, 58.

Kin, M., Tezcan, M., Ileri, Z. & Ayhan, F. (2015). Incidence of white spot lesions among patients treated with self- and conventional ligation systems. Clin Oral Investig, 19, 1501-1506.

Kusgoz, A., Tuzuner, T., Ulker, M., Kemer, B. & Saray, O. (2010). Conversion degree, microhardness, microleakage and fluoride release of different fissure sealants. J Mech Behav Biomed Mater, 594-599.

Kyung-Jin, P., Tessa, K., Uwe, G., Ortrud, Z., Felix, K., Rainer, H. & Dirk, Z. (2019). Effectiveness of caries-preventing agents on initial carious lesions within the scope of orthodontic therapy. Korean J Orthod, 49(4), 246-253.

Leizer, C., Weinstein, M., Borislow, A. J. & Braitman, L. E. (2010). Efficacy of a filled-resin sealant in preventing decalcification during orthodontic treatment. Am J Orthod Dentofacial Orthop, 137, 796-800.

Lussi, A., Hellwig, E. & Klimek, J. (2012). Fluorides-mode of action and recommendations for use. Schweiz Monatsschr Zahnmed, 122, 1030-1042.

Lynch, R. J. & Ten Cate, J.M. (2006). Effect of calcium glycerophosphate on demineralization in an in vitro biofilm model. Caries Res, 40, 142-147.

Mitchell, L. (1992). Decalcification during orthodontic treatment with fixed appliances-an overview.Br J Orthod, 19(3),199-205.

Nascimento, P. L., Fernandes, M. T., Figueiredo, F. E. & Faria-E-Silva, A. L. (2016). Fluoride releasing Materials to Prevent White Spot Lesions around Orthodontic Brackets: A Systematic Review. Braz Dent J, 27(1), 101-107.

Øgaard, B. (1989). Prevalence of white spot lesions in 19-year-olds: a study on untreated and orthodontically treated persons 5 years after treatment. Am J Orthod Dentofacial Orthop, 96, 423-427.

Øgaard, B., Rolla, G. & Arends, J. (1988). Orthodontic appliances and enamel demineralization. Part 1. Lesion development. Am J Orthod Dentofacial Orthop, 94, 68-73.

Oliveira Jr, A. C., Barros, J. V. N., Oliveira, G. J. P. L., Fontanari, L. A., Reis, J. I. L. & Santos, L. M. (2012). O potencial cariostático de quatro diferentes tipos de selantes em dentes bovinos: estudo in vivo. Rev Odontol UNESP, 41(2), 113-117.

Oliveira, C. M. & Sheiham, A. (2004). Orthodontic treatment and its impact on oral health-related quality of life in Brazilian adolescents. J Orthod, 31(1), 20–27.

Paschos, E., Kleinschrodt, T., Clementino-Luedemann, T., Huth, K. C., Hickel, R., Kunzelmann, K. H. & Rudzki-Jansong, I. (2009). Effect of different bonding agents on prevention of enamel demineralization around orthodontic brackets. Am J Orthod Dentofacial Orthop, 135(2), 603-612.

Passalini, P., Fidalgo, T. K. S., Caldeira, E. M., Gleiser, R., Nojima, M. C. G. & Maia, L. C. (2010). Preventive Effect of Fluoridated Orthodontic Resins Subjected to High Cariogenic Challenges. Braz Dent J, 21(3), 211-215.

Pitts, N. B., Zero, D. T., Marsh, P. D., Ekstrand, K., Weintraub, J. A., Ramos-Gomez, F., Tagami, J., Twetman, S., Tsakos, G. & Ismail, A. (2017). Dental caries. Nat Rev Dis Primers. 3, 17030.

Rogers, S., Chadwick, B. & Treasure, E. (2010). Fluoride-containing orthodontic adhesives and decalcification in patients with fixed appliances: A systematic review. Am J Orthod Dentofacial Orthop, 138(1), 390-398.

Sagarika, N., Suchindran, S., Loganathan, S. & Gopikrishna, V. (2012). Prevalence of white spot lesion in a section of Indian population undergoing fixed orthodontic treatment: an in vivo assessment using the visual International Caries Detection and Assessment System II criteria. J Conserv Dent, 15, 104–108.

Salas, C. F. C., Guglielmi, C.A.B., Raggio, D.P. & Mendes, F. M. (2011). Mineral loss on adjacente enamel glass ionomer cements restorations after cariogenic and erosive challenges. Arch Oral Biol, 56, 1014-1019.

Salgado, V. E., Nassur, C., Pomarico, L. & Maia, L. C. (2010). The association of fluoride in the treatment of incipient caries lesions. Rev Bras Odontol, 67(1), 132-136.

Simonsen, R. J. (2011). From prevention to therapy: minimal intervention with sealants and resin restorative materials. J dent, 39(2), 27-33.

Tuncer, C., Tuncer, B. B. & Ulusoy, C. (2009). Effect of fluoride-releasing light-cured resin on shear bond strength of orthodontic brackets. Am J Orthod Dentofacial Orthop, 135(1), 14-16.

Vital, S.O, Haignere-Rubinstein, C., Lasfargues, J. J. & Chaussain, C. (2010). Caries risk and orthodontic treatment. Int Orthod, 63(8), 28-45.

Wiewiora, C., Armbruster, P., Lallier, T. & Ballard, R. (2018). Effectiveness of Vanish XT in Reducing the Development of White Spot Lesions: An In Vitro Study. Oral Health Prev Dent, 16(4), 345-350.

Published

11/09/2021

How to Cite

LEAL, T. P. .; NAVARRO, R. S. .; FREITAS, P. M. de .; SHITSUKA , C. .; SOUZA, H. M. M. de .; CORRÊA, M. D. .; ORTOLANI, C. L. F. . Effects of sealants on in vitro demineralization of enamel surrounding orthodontic brackets. Research, Society and Development, [S. l.], v. 10, n. 11, p. e560101119923, 2021. DOI: 10.33448/rsd-v10i11.19923. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/19923. Acesso em: 19 apr. 2024.

Issue

Section

Health Sciences