Inhibitory Effect of (-)-myrtenol alone and in combination with antifungal agents on Candida spp.
DOI:
https://doi.org/10.33448/rsd-v10i15.22434Keywords:
Monoterpenes; Antifungal activity; Natural products; Alternative therapy.Abstract
The aim of this study was to examine the effects of (-)-myrtenol alone and combined with antifungal agents against Candida spp. The Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration of (-)-myrtenol and fluconazole against C. albicans and C. parapsilosis strains was obtained using CLSI guidelines. Combination of (-)-myrtenol with antifungal drugs was determined by checkboard test. The (-) myrtenol showed MIC ranging from 256 to 512 µg/mL against both species assay. And the MFC was 512 µg/mL, demonstrated nature fungicidal (MFC/MIC < 4). In addition, combination of antifungal agents (amphotericin B and fluconazole) and (-) myrtenol showed synergistic and additive effects on strains assays. Based on these results, the present study demonstrates that (-) myrtenol showed strong fungicide activity against Candida spp. In addition, Combination of antifungal agents and (-) myrtenol reduces the effective concentrations of both the agents with synergistic to additive effects. Therefore, (-) myrtenol has potential to be developed into an antifungal agent.
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Copyright (c) 2021 Bruno Bezerra Cavalcanti; Hermes Diniz Neto; Walicyranison Plinio da Silva-Rocha; Edeltrudes de Oliveira Lima; José Maria Barbosa Filho; Ricardo Dias de Castro; Fabio Correia Sampaio; Felipe Queiroga Sarmento Guerra
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