Moringa oleifera, nutritional, phytochemical and toxicological evaluation of stem, stalk

Authors

DOI:

https://doi.org/10.33448/rsd-v11i6.27682

Keywords:

Moringa oleifera; Nutritional assessment; Phytochemical; Toxicological; Pharmacological.

Abstract

The objective of the present work was to compare the nutritional, phytochemical and toxicological aspects of the stem, stalk and leaf of Moringa oleífera. The methodology used for nutritional assessment was centesimal analysis, with quantification of ash, moisture, lipids, proteins and carbohydrates (Aoac, 2005). Qualitative phytochemical analysis was performed using colorimetric methods to identify alkaloids, tannins, flavonoids, saponins and quinones, while the quantification of total phenolic compounds and flavonoids was performed by spectrophotometry and the antioxidant activity was performed using the DPPH method (2 ,2-diphenyl-1-picrylhydrazyl). The presumptive toxicity test was performed through the lethality in Artemia salina (Mclaughlin, 1991). The nutritional analysis showed that all parts have low moisture values and good ash content, also demonstrating the presence of lipids, proteins and carbohydrates. The sheet obtained more significant numbers in relation to the other parts. The phytochemical analysis showed the presence of alkaloids, tannins and flavonoids. The total phenolics of stem, stalk and leaf showed values of 479.47 mg of GAE/100g, 1830.31 mg of GAE/100g and 1468.97 mg of GAE/100g, respectively, and total flavonoids of 147.80 mg of CAE /100g 331.10 mg of CAE/100g and 343.33 mg of CAE/100g for stem, stalk and leaf, respectively. In the antioxidant test, the stalk showed the best reduction of free radicals (545.51 µmol/L). Finally, in the toxicity tests, both the stem, stalk and leaf were low or showed no toxicity.

References

Alasni Arsad, I. A. A. & Samian, M. R. (2018). Total Phenolics, Total Flavonoids, Antioxidant Capacities, and Volatile Compounds Gas Chromatography-Mass Spectrometry Profiling of Moringa oleifera Ripe Seed Polar Fractions. Phamacognosy Magazine, 14(54), 191-194.

Ali, M. A.; Yusof, Y. A.; Chin, N. L. & et al. (2017). Processing of Moringa leaves as natural source of nutrientes by optimization of drying and grinding mechanism. Journal of Food Process Engineering, 40, 1-17.

Alves, C.Q.; Brandão, H.N.; David, J.M. & et al. (2007). Avaliação da atividade antioxidante de flavonoides. Diálogos & Ciência – Revista da Rede de Ensino, 5(12), 1-8.

ANVISA Agencia Nacional De Vigilância Sanitária. (2010). Farmacopeia Brasileira, v. 1. 5ª Ed. Brasília.

ANVISA Agencia Nacional De Vigilância Sanitária. (2003). Resolução da diretoria colegiada- RDC nº360, 23 de dezembro de 2003.

Anwar, F.; Latif, S.; Ashraf, M. & et al. (2007) Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research, 21(1), 17-25.

Association Of Official Analytical Chemists - AOAC. (2005). Official methods of analysis. Washington.

Barbosa, W. L. R.; Quignard, E.; Tavares, E. C. C. & et al . (2004). Manual para Análise Fitoquímica e Cromatográfica de Extratos Vegetais. Revista Cientifica da UFPA. 4, 3-7.

Bezerra, A. M. E.; Momenté, V. G. & Medeiros Filho, S. (2004). Germinação de sementes e desenvolvimento de plântulas de moringa (Moringa oleifera Lam.) em função do peso da semente e do tipo de substrato. Revista Horticultura Brasileira, 22( 2), 295-299.

Bolzan, R. C. (2013) Bromatologia. Frederico Westphalen, RS: Universidade Federal de Santa Maria, Colégio Agrícola de Frederico Westphalen.

Cabrera-Carrión, J. L.; Jaramillo-Jaramillo, C.; Dután-Torres, F. & et al. (2017) Variación del contenido de alcaloides, fenoles, flavonoides y Taninos en Moringa oleifera lam. En función de su edad y altura. Bioagro, 29(1), 53-60.

Choi, C.W.; Kim, S.C.; Hwang, S.S. & et al. (2002). Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Science, 163, 1161-1168.

Durgesh, K.D.;, Jyotsna, D.; Anil, K. & et al. (2013) Uma árvore multiuso - Moringa oleifera. International Jornal of Pharmaceutical Chemistry, 2, 415-423.

González-Aguilar, G. A.; Villegas-Ochoa, M. A.; Martínez-Téllez, M. A.. & et al. (2007). Improving antioxidant capacity of fresh-cut mangoes treated with UV-C. Journal of Food Science, 72(3), 197-202.

He, T.B., Huang, Y.P., Huang, Y. & et al. (2018). Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera. International Journal of Biological Macromolecules, v. 112, 126-138.

Jaiswal, D.; Rai, P.K.; Kumar, A. (2009). Effect of Moringa oleifera Lam. on hyperglycemic rats. Journal of Ethnopharmacology, v. 123, 392-396.

Kim, D.O.; Lee, K.W.; Lee, H.J. & et al. (2002) Vitamina C equivalente antioxidant capacity (VCEAC) of phenolics phytochemicals. Journal of Agricultural and Food Chemistry, 50, 3713-3717.

Kwaambwa, H.; Maikokera, R. (2008). Infrared and circular dichroism spectroscopic characterisation of secondary structure components of a water tratment coagulnt proteinextracted from Moringa oleiferaseeds. Colloids and Surfaces B: Biointerfaces, 64, 118-125.

Leone, A.; Spada, A.; Battezzati, A. & et al. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International Journal of Molecular Sciences, 16, 12791–12835.

Leone, A.; Bertoli, S.; Bi Bello, S. & et al. (2018). Effect of Moringa oleifera Leaf Powder on Postprandial Blood Glucose Response: In Vivo Study on Saharawi People Living in Refugee Camps. Nutrients, 10(1494), 1-14.

Lin, H.; Zhu, H.; Tan, J. & et al. (2019). Comparative Analysis of Chemical Constituents of Moringa oleifera Leaves from China and India by Ultra-Performance Liquid Chromatography Coupled with Quadrupole-Time-Of-Flight Mass Spectrometry. Molecules, 24(942), 1-25.

Mahmood, K.; Mugal, T.; Haq, I.U. (2010). Moringa oleifera: A natural gift-A review. Journal of Pharmaceutical Sciences and Research, 2, 775–78.

Matos, F.J.A. (1997) Introdução à Fitoquímica experimental. Ceará: Fortaleza, UFC.

Mclaughlin, J.L. (1991). Crowgall tumors in potato discs and brine shrimp lethality: two simple bioassays for higher plant screening na fractionation. In: methods in plant biochemistry, hostettman, k. (Ed.). Academic Press, London.

Menezes, F. S., Minto, A.B.M., Ruelas, H.S. & et al. (2007). Hypoglycemic atividade de duas espécies brasileiras Bauhinia: Bauhinia forficata L. e Bauhinia monandra Kurz. Revista Brasileira de Farmacognosia, 17, 8-13.

Mensah, J.K.; Ikhajiagbe, B.; Edema, N.E. & et al. (2012) Phytochemical nutritional and antibacterial properties of dried leaf powder of Moringa oleifera (Lam) from Edo Central Province, Nigeria. Journal of Natural Product and Plant Resources, 2, 107-112.

Mohamed, A. A. R.; Metwally, M.M. Khalil, S. R. R. & et al. (2019) Moringa oleifera extract attenuates the CoCl2 induced hypoxia of rat's brain: Expression pattern of HIF-1α, NF-kB, MAO and EPO. Biomedicine & Pharmacotherapy, 109, 1688–1697.

Monteiro, J.M.; Albuquerque, U.P.; Araujo, E. L. (2006). Taninos: uma abordagem da química à ecologia. Química nova, 28(5), 892-896.

NWIDU, L.L.; EMORSY, E.; APRIOK, J.S. & et al. (2018). In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria. Medicines, 5(71),1-17.

Ozcan, T.; Akpinar-Bayizit, A.; Yilmazersan, L. & et al. (2014). Phenolics in human health. International Journal of Chemical Engineering and Applications, 5, 393-396.

Paikra, B. K.; Dhongade, H. K. J.; Gidwane, B. (2010). Phytochemistry and Pharmacology of Moringa oleifera Lam. Journal of Pharmacopuncture, 20(3), 194-200.

Pedral, A. L.; Barbosa, J. S.; Santos, G. R. & et al. (2015).Caracterização físico-química de folhas da Moringa oleífera desidratadas por secagem convectiva e liofilização. Revista Brasileira de Produtos Agroindustriais, 17(1), 33-39.

Rossi, J.A; Singleton, V.L. (1965). Colorimetry of total phenolics with phosphomolybidic-phosphotungstic acid reagente. American Journal of Enology and viticulture, 16, 144-158.

Rufino, M. S. M.; Alves, R. E.; Brito, E. S. & et al. (2010). Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chemistry, 121, 996-1002.

Saini, R.K.; Manoj, P.; Shetty, N.P. & et al. (2016) Biodisponibilidade relativa de folato da planta de alimento tradicional Moringa oleifera L. como avaliado em um modelo de rato. Journal of Food Science and Technology, 53, 511-520.

Saini, R.K.; Shetty, N.P.; Giridhar, P. (2014). GC-FID / MS análise de ácidos graxos em cultivares indianas de Moringa oleifera : fontes potenciais de PUFA. Journal of the American Oil Chemists' Society, 91, 1029-1034.

Santos, A. R. F. (2010). Desenvolvimento Inicial de Moringa oleífera Lam. Sob Condições de Estresse. Dissertação de mestrado. Universidade Federal de Sergipe. Sergipe.

Saraiva, L.C.F.; Maia, W. M. N.; Leal, F. R. & et al. (2018) Triagem fitoquímica das folhas de Moringa oleifera. Boletim Informativo Geum, 9(2), 12-19.

Silva, M. V. S.; Padilha, R. T.; Padilha, D. M. M. (2021). Benefícios da Moringa oleifera para saúde humana e animal: Revisão de Literatura. Research, Society and Development, 10(8), 1-10.

Simões, C. M. O.; Schenkel, E. P.; Gosmann, G. & et al.(2011). Farmacognosia: da planta ao medicamento. 2 edição. Porto Alegre: Editora Universidade/UFRGS.

Silva, S. L.; Nascimento, A.A.; Ribeiro, E.F.B. & et al. (2016). Avaliação da toxicidade aguda pré-clínica do extrato metanólico das cascas do caule de Parahancornia amapa (Apocynaceae). Acta Amazonica, 46(1), 73-80.

Tiwari, A. B. P.; Bahu, P. K.; Kumar, S. A. (2018). Review of the Phytochemical and Pharmacological Characteristics of Moringa oleifera. Journal of Pharmacy & Bioallied Sciences, 10(4), 181-191.

Vieira, G. F. (2017). Determinação de macro e micro nutrientes de frutos de Morianga oleífera Lamark (parede interna e externa da casca) e sementes. Dissertação de mestrado. Universidade Federal do Rio Grande do Norte. Natal.

Published

25/04/2022

How to Cite

SANTOS, M. L. dos .; OLIVEIRA, A. P. da S. .; SANTOS, B. N. G. dos .; SILVA, O. A. .; NUNES, L. C. C. Moringa oleifera, nutritional, phytochemical and toxicological evaluation of stem, stalk . Research, Society and Development, [S. l.], v. 11, n. 6, p. e19511627682, 2022. DOI: 10.33448/rsd-v11i6.27682. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/27682. Acesso em: 19 apr. 2024.

Issue

Section

Health Sciences