Influence of carbon source, agitation and aeration rates for production yeast biomass which potential of use for biological control
DOI:
https://doi.org/10.33448/rsd-v9i4.3066Keywords:
biocontrol; microbial biomass; fungi; fermentation; industrial bioprocess.Abstract
The fermentation processes are used to promote better results when it is desired to increase the biomass of organisms that can be used in biological control. Thus, the aim of this work was to expand the scale of production of biomass of yeasts that have potential for biological control. A total of five strains of yeasts isolated from natural microflora of tropical fruits, a strain was selected and evaluated for the influence of the carbon source, cultivation orbital shaker and bioreactor. The tests performed demonstrate that the use of invert sugar as carbon source favored production of biomass material when compared to that obtained with glucose. The bioreactor cultivation of the yeast strain has enhanced performance, since it favors an increase of 24.12% (6.47 to 8.03 g.L-1) in biomass production when compared to the fermentation orbital shaker and that may be associated with increased agitation and aeration rate.
References
Bendo, M. I.; Viecelli, C. A. (2009). Controle biológico de Rhizopus nigricans em pós-colheita de morango pela utilização da levedura Saccharomyces cerevisiae em leite in natura. Cascavel, v.2, n.3, p.23-35.
Chan, Z.; Tian, S. (2005). Interaction of antagonistic yeasts against post-harvest pathogens of apple fruit and possible mode of action. Postharvest Biology Technology, v. 36, n. 2, p. 215-223.
De Leon, C. A. P.; Bayon, M. M. (2002). Selenium incorporation into Saccharomyces cerevisae cells: A study of different incorporations methods. Journal Applied of Microbiolgy, v. 92, n.4, p. 602-610.
Difco & BBL Manual. (2009). Manual of Microbiological Culture Media. Second Edition. Disponível em: http://www.bd.com/ds/technicalCenter/misc/difcobblmanual_2nded_lowres.pdf. Acesso em: 12.05.2018
Droby, S.; Wisniewski, M.; Macarisin, D.; Wilson, C. (2009). Twenty years of postharvest biocontrol research: is it time for a new paradigm? Postharvest Biology Technology, v. 52, n. 2, p.137-145.
Gomes, T. M. C. (2010). Produção de Hidromel: efeito das condições de fermentação. Dissertação de Mestrado. Escola Superior Agrária de Bragança. Bragança, p.76.
Gouvea, A. (2007). Controle em campo e pós-colheita de doenças e metabolismo do morangueiro após tratamento com Saccharomyces cerevisiae. Tese de Doutorado. Universidade Federal do Paraná. Curitiba.
Janisiewicz, W. J.; Tworkoski, T.J.; Sharer, C. (2000). Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients. Phytopathology, v. 90, n.11, p. 1196-1200.
Kurzman, C. P.; Fell, J. W. (1998). The yeasts: a taxonomic study. 4. Ed. New York: Elsevier, 1074 p.
Malta, H. L. (2006). Estudos de parâmetros de propagação de fermento (Saccharomyces cerevisiae) para produção de cachaça de alambique. Dissertação de Mestrado. Universidade Federal de Minas Gerais. Belo Horizonte – MG.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analitycal Chemistry, v, 31, p. 426.
Moraes, D. C.; Bianchi, V. L. D. (2013). Avaliação da produção de etanol a partir de glicose e xilose através de batelada alimentada por leveduras isoladas da casca de uvas (vitis spp). Analytica, v. 62, p. 38.
Neves, L. C. M. (2003). Obtenção da enzima glicose 6-fosfato desidrogenase utilizando Saccharomyces cerevisiae W303-181. Dissertação de Mestrado. Faculdade de Ciências Farmacêuticas. Universidade de São Paulo. São Paulo.
Nitschke, M.; Rodrigues, V.; Schinatto, L. F. (2001). Formulação de meios de cultivo a base de soro de leite para produção de goma xantana por Xanthomonas campestris C7L, Ciência e Tecnologia de Alimentos, v. 21, p. 82-85.
Rehm, H.J.; Reed, G.: Pühler, A.; Stadler, P. (9195). Biotechnology: a multi volume comprehensive treatise. 2. Ed., vol. 9. Enzymes, Biomass, food and feed. ed. By G. Reed and T.W Nagodawithana. Cap. 5. Production of Microbial Biomass.
Reifenberg, E.; Boles, E.; Ciriacy, M. (1997). Kinetic characterization of individual hexose transporters of Saccharomyces cerevisae and their regulations to the triggering mechanism of glucose repression. European Journal of Biochemistry, v.245, n. 2, p.324-333.
Schrank, A.; Franceschini, M.; Guimarães, A.P.; Camassola, M.; Frazzon, A. P.; Baratto, C, M.; Kogler, V.; Silva, M, V.; Dutra, V.; Nakazonto, L.; Castro, L.; Santi, L.;Vainstein, M. H. (2001). Biotecnologia aplicada ao controle biológico - O entomopatógeno Metarhizium anisopliae – Biotecnologia, Ciência e Desenvolvimento, nº 23.
Silva, D, P.; Pessoa JR, A.; Roberto, I. C.; Vitolo, M. (2001). Effect of agitation and aeration on production of hexokinase by Sacharomyces cerevisae. Applied Microbiology Biothecnology, v. 91, n.3, p. 605-613.
Silveira, N.S.S.; Michereff, S.J.M.; Silva, I.L.S.S.; Oliveira, S.M.A. (2005). Doenças fúngicas pós-colheita em frutas tropicais: patogênese e controle. Revista Caatinga, v.18, n.4, p.283-299.
Souza, C. S. (2009). Avaliação da produção de etanol em temperaturas elevadas por uma linhagem de S. cerevisiae. Tese de Doutorado. Programa de Pós-Graduação Interunidades em Biotecnologia (USP). Instituto Butantan (IPT), São Paulo.
Taccari, M.; Canonico, L.; Comitini, F.; Mannazzu, I.; Ciani, M. (2012). Screening of yeasts for growth on crude glycerol and optimization of biomass production. Bioresource Technology, v.110, p.488-495.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
