Selection of technique for yeast collection from the canine oral cavity
DOI:
https://doi.org/10.33448/rsd-v11i8.30716Keywords:
Comparative Study; Sample Collection; Microbiota; Experiment.Abstract
The first step in the collection of microbiological samples is the selection of techniques to be used, which guarantees a higher yield of microorganisms and a more assertive identification of them. Considering the scarcity of studies related to the presence of yeasts in the canine oral cavity, this work broached the comparison between techniques of collection of the canine oral cavity already used and not yet used in 18 dogs treated at the Hospital Veterinário de Animais de Companhia (UFRRJ) and private clinics of the adjacencies. The purpose of this comparative study is to stimulate research for the standardization of the canine oral microbiome and to expand knowledge about the yeasts present in this microbiota. Therefore, the following instruments were compared for sample collection: swab, filter paper and commercial human toothbrush. The technique that demonstrated the greater use of the sample and less microbial loss was the use of a toothbrush. Therefore, it was concluded that the most effective collection of yeasts from the canine oral cavity was the commercial human toothbrush, recommended for future trials.
References
Agarwal, S.; Manchanda, V.; Verma, N. & Bhalla, P. (2011). Yeast identification in routine clinical microbiology laboratory and its clinical relevance. Indian Journal of Medical Microbiology, 29 (2), 172.
Arias, M. V. B.; Battaglia L. A.; Aiello, G.; Carvalho, T. T. & Freitas, J. C. (2008). Identificação da suscetibilidade antimicrobiana de bactérias isoladas de cães e gatos com feridas traumáticas contaminadas e infectadas. Semina: Ciências Agrárias, 29 (4), 1-15.
Barrios, C. L.; Lopez, C. B.; Pavletic, C.; Parra, A.; Vidal, M.; Bowes, J. & Fatjó, J. (2021). Epidemiology of dog bite incidents in Chile: factors related to the patterns of humandog relationship. Animals, 11 (1), 96.
Bringel, M.; Jorge, P. K.; Francisco, P. A.; Lowe, C.; Sabino-Silva, R.; Colombini-Ishikiriama, B. L.; Machado, M. A. A. M. & Siqueira, W. L. (2020). Salivary proteomic profile of dogs with and without dental calculus. BMC Veterinary Research, 16 (1), 1-12.
Ciurea, C. N.; Kosovski, I. B.; Mare, A. D.; Toma, F.; Pintea-Simon, I. A. & Man, A. (2020). Candida and Candidiasis – Opportunism Versus Pathogenicity: A Review of the Virulence Traits. Microorganisms, 8 (6), 857.
Forsblom, B.; Love, D. N.; Kessel E. S. & Somer, H. J. (1997). Characterization of anaerobic, Gram-negative, nonpigmented, saccharolytic rods from subgingival sites in dog. Clinical Infectious Diseases, 25 (2), 100-106.
Frias, D. F. R.; Lages, S. L. S. & Carvalho, A. A. B. (2011). Avaliação da conduta de profilaxia antirrábica humana indicada para pessoas envolvidas em agravos com cães e gatos no Município de Jaboticabal, SP, no período de 2000 a 2006. Revista Brasileira de Epidemiologia, 14 (4), 722-732.
Gaviria, M. G. & Montes, H. M. M. (2020). Current Aspects in the Biology, Pathogeny, and Treatment of Candida krusei, a Neglected Fungal Pathogen. Infection and Drug Resistance, 13, 1673.
Goldstein, E. J. C. (1992). Bite Wounds and Infection. Clinical Infectious Diseases, 14 (3), 633-640.
Harvey, C. E.; Laster, L.; Shofer, F. & Miller, B. (2008). Scoring the Full Extent of Periodontal Disease in the Dog: Development of a Total Mouth Periodontal Score (TMPS) System. Journal of Veterinary Dentistry, 25 (3), 176-180.
Henton, J. & Jain, A. (2012). Cochrane corner: antibiotic prophylaxis for mammalian bites (intervention review). Journal of Hand Surgery, 37 (8), 804-806.
Junior, D. P. L.; Costa, G. L.; Dantas, E. S. O.; Nascimento, D. C.; Moreira, D.; Pereira, R. S.; Ramos, R. T. B.; Bonci, M. M.; Maia, M. L. S.; Gandra, R. F.; Auler, M. E.; Melhem, M. S. C. & Paula, C. R. (2020). The Rise of Fungi: Evidence on the Global Scale. Old Know Silences or Mysterious Threats to the Planet. Microbiology Research Journal International, 30 (10), 18-49.
Kurtzman, C. P; Fell, J. W. & Boekhout, T. (2011). The Yeasts – a taxonomic study. 5 ed. USA: Elsevier Science.
Macedo, J. L. S.; Camargo, L. M. & Almeida, P. F. (2006). Estudo prospectivo do fechamento primário das mordeduras caninas e humanas na face e no couro cabeludo. Revista da Sociedade Brasileira de Cirurgia Plástica, 21 (1), 23-29.
Montrose, V. T.; Squibb, K.; Hazel, S.; Kogan, L. R. & Oxley, J. A. (2020). Dog bites dog: The use of news media articles to investigate dog-on-dog aggression. Journal of Veterinary Behavior, 40, 7-15.
Omran, A.; Jordan, C. & Huq, S. (2020). Appropriate antibiotics in dog bite injuries: an audit study. International journal of surgery: Short reports, 5 (2), 9.
Paula, C. R.; Navarro, B. S.; Bonci, M. M. & Nascimento, D. C. (2021). Importance of Yeasts in Oral Canine Mucosa. Canine Medicine. www.intechopen.com/online-first/importance-of-yeasts-in-oral-canine-mucosa. Acessado em: 8 de maio de 2021.
Pereira, G. S.; Carvalho, M. R.; Abdalla, S. L.; Silva, M. F. A.; Gomes, L. G.; Spiller, P. R.; Faria, E. F.; Dall’Acqua, P. C.; Paula, E. M. N. & Martini, A. C. (2021). Detecção de dois tipos tumorais distintos em cavidade bucal de Cão: Relato de caso. Research, Society and Development, 10 (6), 1-7.
Rui-Feng, C.; Li-Song, H.; Ji-Bo, Z. & Li-Qiu, W. (2013). Emergency treatment on facial laceration of dog bite wounds with immediate primary closure: a prospective randomized trial study. BMC Emergency Medicine, 13 (1), 1-5.
Santin, R. (2009). Isolamento, identificação e suscetibilidade in vitro de leveduras isoladas da cavidade oral de fêmeas caninas (Dissertação de Mestrado em Medicina Veterinária), Universidade Federal de Pelotas, Rio Grande do Sul, Brasil.
Tulloch, J. S. P.; Garstecka, S. C. O.; Fleming, K. M; Vivancos, R & Westgarth, C (2021). English hospital episode data analysis (1998–2018) reveal that the rise in dog bite hospital admissions is driven by adult cases. Scientific reports, 11 (1), 1-12.
Venturini, M. A. F. A. (2006). Estudo retrospectivo de 3055 animais atendidos no ODONTOVET (Centro Odontológico Veterinário) durante 44 meses (Dissertação de Mestrado em Medicina Veterinária). Universidade de São Paulo, São Paulo, Brasil.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Clara de Almeida Mendes; Mário Mendes Bonci; Francisco de Assis Baroni
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
