Morpho-cultural, molecular characterization and identification of the pathogen of the burning of leaf and cashew fruit
DOI:
https://doi.org/10.33448/rsd-v11i9.31431Keywords:
Cashew; Burning of the leaf and fruit of cashew tree; Mozambique.Abstract
The cashew tree (Anacardium occidentale L.) is one of the most important fruit trees cultivated in the semi-arid regions of tropical climate in the world. However, this crop is affected by numerous diseases throughout its production cycle. The burning of the leaf and fruit of cashew is a relatively new disease in Mozambique and is characterized by causing necrotic lesions in fruits and leaves. Thus, the present work was carried out with the objective of isolating and making the morphological and molecular characterization of the fungus responsible for burning the leaf and fruit of the cashew tree and determining its taxonomic identity. The study was based on laboratory tests with the aid of the optical microscope and polymerase chain reaction (PCR). In PDA, cultures of spongy dark coloration were often isolated and distinguished; abundant orange and white and, spongy, typified as Neofusicoccum spp., Colletotrichum spp. and Pestalotiopsis spp., respectively. Molecularly, the results confirmed that at least these genera of fungi are involved in the disease of leaf burning and cashew fruit. No Cryptosporiopsis spp. isolates were isolated. However, it is recommended to evaluate the interactive/synergistic effect of the different isolates obtained from samples with symptoms of leaf burning and cashew fruit in the different stages of host tissue development.
References
Altschul, S. F. (1990) ‘Basic local alignment search tool’, Journal of Molecular Biology, 215(3), 403–410. doi: 10.1016/S0022-2836(05)80360-2.
Araújo, J. P. P. (2013). Agronegócio Caju prática e inovações. Embrapa Agro-indústria Tropical. Embrapa: Brasília.
Cardoso, J. E. & Freire, F.C. O. (2002). Identification and management of the main diseases. Cashew plant health. Embrapa Technological Information. Brasilia.
Cardoso, J. E.; Santos, A.A.; Heifer, M. A.; Souza Neto, J. & Souza, R. N.M. (2005). Epidemiology of black mold and damage to cashew production. Fortaleza, Embrapa.
Cardoso, J. E.; Viana, F.M. P. & Martins, M. V. V. (2018). Diseases Caused by Fungi of the Family Botryosphaeriaceae in Cajueiro. Circular Technique 47. Embrapa. Fortress, CE.
Carollo, E. M. & Santos Filho, H. P. (2016). Manual Básico de Técnicas Fitopatológicas Laboratório de Fitopatologia. Embrapa Mandioca e Fruticultura. Ministério da Agricultura, Pecuária e Abastecimento Brasília, DF.
Carvalho, D. D. C.; Alves, E.; Batista, T. R.S.; Camargo, R. B. & Lopes, E. A. G. L. (2008). Comparison of methodologies for conidia production by. Alternaria from citrus. Brazilian Journal of Microbiology. 39, 792-798.
Cordeiro, A. B.; Gonçalves, J. S.; Lopes da Silva, M. R.; Marçal, V. V. M. & Leite Júnior, R. P. (2007). Caracterização morfológica e cultural de isolados de Colletotrichum spp. obtidos de Cafeeiros no estado do Paraná. VI Simpósio de Pesquisa dos Cafés do Brasil.
Coutinho, I. B.L.; Cardoso, J. E.; Lima, C. S.; Lima, J. S.; Gonçalves, F. J. T.; Silva, A. M. S. & Freire, F. C. O. (2018). An emended description of Neofusicoccum brasiliense and characterization of Neoscytalidium and Pseudofusicoccum species associated with tropical fruit plants in northeastern Brazil. Article. Phytotaxa, 358(3), 251–264.
Coutinho, I.B. L. (2016). Molecular characterization and control of fungi of the family Botryosphaeriaceae associated with tropical fruit trees. PhD thesis in Agronomy/Phytotechnics. Center of Agrarian Sciences, Federal University of Ceará, Fortaleza.
Diniz, I.; Monteiro, F.; Catarino, L.; Batista, D. (2021). Manual de Laboratório para Realização de Isolamento de Fungos e Bactérias. Apoio à Luta Contra Doenças e Pragas do Cajueiro na Guiné-Bissau. FAO, Lisboa.
Dominic, M.; Makobe M.; Agboton B.; Shomari S. & Tiedemann A. (2014). Biology and Infection Mechanisms of Cryptosporiopsis spp Fungus Causing Blight Disease on Cashew (Anacardium Occidentale L.). Journal of Plant Sciences, 2(6), 266-275.
Edgar, R. C. (2004) ‘MUSCLE: multiple sequence alignment with high accuracy and high throughput.’, Nucleic acids research, 32(5), 1792–7. doi: 10.1093/nar/gkh340.
Fernandez, M. R. (1993). Manual for laboratory of phytopathology. Bottom Step: Embrapa-CNPT. p. 128.
Figueirêdo, L.C. (2005). Genetic Variability Analysis of Colletotrichum Gloeosporiodes isolates from Cajueiro (Anacardium Occidentale L.) Using Molecular Markers and Pathogenicity Test. Master's thesis. Federal University of Pernambuco. Center for Biological Sciences, Department of Mycology. Recife-PE.
Freire, F. C. O.; Cardoso, J. E.; Dos Santos, A. A. & Viana, F. M. P. (2002). Diseases of cashew nut plants (Anacardium occidentale L.) in Brazil. Crop Protection 21, 489-494.
Freire, F.C. O. & Cardoso, J. E. (2003). Cashew diseases. pp. 192-225 in: Diseases of tropical fruit trees of agro-industrial interest. Freire, F.C.O., Cardoso, J.E., Viana, F.M.P. (eds.). Embrapa Technical Information. Brasilia.
Garrido, L. da R. & Gava, R. (2014). Manual of Fungal Diseases of the Vine. Bento Gonçalves RS. Embrapa Grape and Wine, Brazil.
Instituto de Fomento de Caju (INCAJU) (2007). Report Balance of Activities Carried out by the Cashew sector from 1999 to 2005. Ministry of Agriculture. Maputo - Mozambique.
Intini, M. & Sijaona, M. E. R. (1983). Little Known Disease of Cashew (Anacardium occidentale L.) in Tanzânia. Revista de Agricultura Subtropicale e Tropicale 77, 421-429.
Jeewon, R.; Liew, E.C.Y. & Hyde k.D. (2004). Phylogenetic evaluation of species nomenclature of Pestalotiopsis In relation to host association. Fungal Diversity 17, 39-55.
Kruschewsky, M.C. (2010). Taxonomy and ecology of the genus Pestalotiopsis in Brazil, with emphasis on the Atlantic Forest of southern Bahia. MSc (Plant Production). Master's dissertation in Plant Production at the State University of Santa Cruz, Brazil.
Lima, J.S.; Moreira, R.C.; Cardoso, J. E.; Martins, M. V. V. & Viana, F.M. P. (2013). Cultural, morphological and pathogenic characterization of Lasiodiplodia theobromae associated with tropical fruits. Phytopathologica Summa. 39 (2), 81-88.
Lutz, B.S.; Da Silva, G. K.; Corbelini, V.A.; Renner, J.D.P.; Possuelo, L. & Valim, M. A. R. (2013). Standardization of conventional PCR and real-time PCR techniques for the diagnosis of Candida albicans. Revista Jovens Pesquisadores, Santa Cruz do Sul, 3(1), 51-66.
Mafacioli, R.; Tessman, D.J.; Santos, A. F. & Vida, J.B. (2006). Morphophysiological characterization and pathogenicity of Colletotrichum gloeosporioides of pupunheira. Phytopathological Summa, 32.
Majune D. J.; Masawe P. A. L.; Kafiriti E. & Mbega E. R. (2019). Detection and characterization of cashew leaf and nut blight disease in the Eastern and the Southern Zones of Tanzânia. Research Journal of Agriculture and Forestry Sciences, 7(2), 36-44.
Marques, M. W.; Lima, N. B.; Morais Jr, M. A.; Michereff, S. J.; Phillips, A. J. L. & Câmara, M. P. S. (2013). Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil. Fungal Diversity.61, 95–208.
Masawe, P. A. L.; Kafiriti, E. M.; Mneney, E. E.; Shomari, S. H.; Hullaya, A. K.; Kasunga, I. J. F.; Bashiru, R. A.; Kanbaza, A. & Kidunda, B. (2015). Proceedings of the Third International Cashew Conference. Cashew For Health Wealth and Environment. Dar Es Salaam, Tanzânia, 16-19th.
Menezes, M. (2006). Biological and Taxonomic Aspects of Species of the Genus Colletotrichum. Anais of the Pernambuco Academy of Agronomic Science, Recife, 3, 170-179.
Micheriff, S. J. 2001. Fundamentos de Fitopatologia. Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Área de Fitossanidade, Recife – PE, Brasil
Mills, P. R.; Sreenivasaprasad, S. & Brown, A. E. (1992). Detection and differentiation of Colletotrichum Gloeosporioides isolates using PCR. Federation of European Microbiological Societies (FEMS) Microbiology Letters 98, 137-144.
Morgulis, A. (2008) ‘Database indexing for production MegaBLAST searches’, BIOINFORMATICS ORIGINAL PAPER, 24(16), pp. 1757–1764. doi: 10.1093/bioinformatics/btn322.
Mulandane, F. C.; Fafetine, J.; Abbeele, J. V. D.; Clausen, P. H.; Hoppenheit, A.; Cecchi, G.; Oosthuizen, M.; Delespaux, V. & Neves, L. (2018). Resistance to trypanocidal drugs in cattle populations of Zambezia Province, Mozambique. Parasitology Research 117, 429–436.
NCBI (2013) National Center for Biotechnology Information (NCBI). Available at: http://www.ncbi.nlm.nih.gov/.
Old, K. M.; Dudzinski, M. J.; Pongpanich, K.; Yuan, Z. Q.; Pham, Q. T. & Nguyen, T. N. (2002). Cryptosporiopsis leaf spot and shoot blight of eucalypts. Australasian Plant Pathology, 31, 337-344.
Ramos, G. de S. (2021). Caracterização de Diferentes Espécies de Bipolaris e Exserohilum em Linhagens de Milho-pipoca. Tese de Doutoramente na Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes – Rio de Janeiro, Brasil.
Serra, I.M. R. S. & Coelho, R. S.B. (2007). Pestalotiopsis staining in heliconia: characterization of the disease and potential sources of resistance. Brazilian phytopathology 32, 44-49.
Sijaona, M. E. R.; Reeder, R. H. & Waller, J. M. (2005). Cashew leaf and nut blight – A new disease of cashew in Tanzânia caused by Cryptosporiopsis spp. Disponível em Acesso http://www.bspp.org.uk/ndr/asp.
Sobrinho, C. A.; Da Silva, P. H. S.; De Sousa, H. U.; Santos, F. J. S.; Rodrigues, B. H. N. (2021). Monitoramento de Doenças Fúngicas do Cajueiro em Consórcio com Abacaxi, com Feijão-Caupi e com Mandioca no Estado do Maranhão. Boletim de Pesquisa e Desenvolvimento 139. Embrapa Meio-Norte.
Tavaré, S. (1986) ‘Some probabilistic and statistical problems in the analysis of DNA sequences’, American Mathematical Society: Lectures on Mathematics in the Life Sciences, 17, 57–86. doi: citeulike-article-id:4801403.
Tozze Junior, H. J.; Firmino, A.C.; Fischer, I.H.; Furtado, E. L. & Massola Júnior, N. S. (2015). Characterization of Colletotrichum spp isolates. Associated with fruit trees in the State of São Paulo. Summa Phytopathologica, 41(4), 270-280.
Uaciquete, A. (2013). Characterization, epidemiology and control strategies for the anthracnose pathogen (Colletotrichum spp.) on cashew (Anacardium occidentale L.) in Mozambique. Ph.D. (Plant Pathology) thesis. In the Faculty of Natural and Agricultural Sciences Department of Microbiology and Plant Pathology University of Pretoria, Pretoria.
Veloso, J. S.; Duarte, I. G.; Farias, O. R. Câmara, M. P. S. (2022). Antracnose do Cajueiro: Etiologia, Sintomatologia e Aspectos Epidemiológicos. Revisão Anual de Patologias de plantas, Volume 28.
Vivek, M. N.; Manasa, M.; Pallavi, S.; Sachidananda Swamy, H. C. & Prashith Kekuda, T. R. (2013). Antibacterial potential of cashew apple (Anacardium occidentale L.) juice against clinical isolates of Staphylococcus aureaus and Staphylococcus mutans. Short communication. Science, Technology and Arts Research Journal 2, 144-146.
Viana, Í. E. T; Martins, M. V. V; Fonseca, W. L.; Lima, C. S.; Araújo, F. S. A. (2020). Comportamento de Clones de Cajueiro-anão ao Oídio e o Dano em Flores e Maturis. Boletim de Pesquisa e Desenvolvimento 199. Fortaleza, CE, Embrapa.
White, T. J., Bruns, T., Lee, S. & Taylor J. (1990). Amplification And Direct Sequencing of Fungal Ribosomal RNA Genes For Phylogenetics. PCR Protocols: A guide to Metthods and Applications. 38, 315 – 322.
Wonni, I.; Sereme, D.; Ouedraogo, I.; Kassankagno, A. I.; Dao, I.; Ouedraogo, L. & Nacro, S. (2017). Diseases of cashew nut plants (Anacardium Occidentale L.) in Burkina Faso. Advances in Plants & Agriculture Research, 6(3),78‒83.
Zhongrun, Z. & Masawe, P. A. L. (2014). Diseases and insect pests of cashew in Tanzânia. Tropical Crops Genetic Resouces Institute – CATAS, China Naleendele agricultural Research Institute – Tanzânia.
Zhu, L.; Wang, X.; Huang, F.; Zhang, J.; Li, H.; Ding, D. & Hyde, K. D. (2012). A destructive new disease of Citrus in China caused by Cryptosporiopsis citricarpa sp. nov. Plant Disease, 96(6), 804-812.
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