The genotypic profile of milk proteins in Holstein cows raised in South of Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v11i9.31530

Keywords:

Allele frequency; Casein; Lactoglobulin; Milk yield.

Abstract

Genotyping in dairy cattle allows knowing the alleles and genotypes associated with milk proteins that affect yield and milk quality. This study aimed to verify the genotype profile for κ-casein I and II (κ-CN), α-s1-casein (α-CN) and β-lactoglobulin (β-LG), for Holstein cows raised in southern Brazil, where there is no scientific evidence on the presence of these alleles in dairy herds. Hair follicles samples from each animal were used for DNA extraction and genotyping. We calculated the allelic and genotypic frequencies for four types of milk protein variants in the population, and a chi-square test was employed to see whether the population of animals used was in Hardy-Weinberg equilibrium. Allele A and AA genotype showed the highest frequency for both κ-CN (56.77 to 80.36%). For α-s1-CN and β-LG, the B allele was the most frequent, as BB (80.26%) and AB (47.15%), respectively. Due to the presence of alleles associated with protein levels in milk, we concluded that these herd cows present milk technological properties interesting to produce dairy products.

Author Biographies

Fabrício Pilonetto, University of São Paulo

Department of Animal Science, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, Brazil.

Giovanni Coelho Ladeira, University of Florida

Department of Animal Science, University of Florida, Gainesville, Florida, United State.

Mayara Salvian, University of São Paulo

Department of Animal Science, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, Brazil.

Aline Zampar, University of Santa Catarina State

Department of Animal Science, University of Santa Catarina State, Chapecó, Santa Catarina, Brazil.

Diego de Córdova Cucco, University of Santa Catarina State

Department of Animal Science, University of Santa Catarina State, Chapecó, Santa Catarina, Brazil.

References

Bedere, N., & Bovenhuis, H. (2017). Characterizing a region on BTA11 affecting β-lactoglobulin content of milk using high-density genotyping and haplotype grouping. BMC Genetics, 18(17), 1-9.

Bittante, G., Cipolat-Gotet, C., & Cecchinato A. (2013). Genetic parameters of different measures of cheese yield and milk nutrient recovery from an individual model cheese-manufacturing process. Journal of Dairy Science, 96(12), 7966-7979.

Bonfatti, V., Di Martino, G., Cecchinato, A., Vicario, D., & Carnier, P. (2010). Effects of β-κ-casein (CSN2-CSN3) haplotypes and β-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows. Journal of Dairy Science, 93(8), 3797-3808.

Brotherstone, S., & Goddard, M. (2005). Artificial selection and maintenance of genetic variance in the global dairy cow population. Philosophical Transactions of the Royal Society, 360, 1479-1488.

Cecchinato, A., Albera, A., Cipolat-Gotet, A., Ferragina, A., & Bittante, G. (2015). Genetic parameters of cheese yield and curd nutrient recovery or whey loss traits predicted using Fourier-transform infrared spectroscopy of samples collected during milk recording on Holstein, Brown Swiss, and Simmental dairy cows. Journal of Dairy Science, 98(7), 4914-4927.

Doublet, A. C., Fritz, C. S., Michelet, A., Hozé, C., Danchin-Burge, C., Laloe, D., & Restoux, G. (2019). The impact of genomic selection on genetic diversity and genetic gain in three French dairy cattle breeds. Genetics Selection Evolution, 51:52, 1-13.

Gouda, E. M., Halall, M. K., & Abdelaziz, S. A. (2013). Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP. Journal of Dairy Science, 5(2), 197-203.

Gustavsson, F., Buitnhuis, A. J., Johansson, M., Bertelsen, H. P., Glantz, M., Poulsen, N. A., Mansson, H. L., Stallhammar, H., Larsen, L. B., Bendixen, C., Paulsson, M., & Andrén, A. (2014). Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. Journal of Dairy Science, 97(6), 3866-3877.

Hallén, E., Wedholm, A., Andrén, A., & Lundén, A. (2008). Effect of b-casein, j-casein and b-lactoglobulin genotypes on concentration of milk protein variants. Journal of Animal Breeding and Genetics, 125, 119-129.

INMET - Instituto Nacional de Meteorologia. (2022). Banco de dados Meteorológicos. https://portal.inmet.gov.br/.

Oner, Y., & Elmaci, C. (2006). Milk protein polymorphisms in Holstein cattle. International Journal of Dairy Technology, 59(3), 180-182.

Perna, A., Intaglietta, I., Gambocorta, E., & Simonetti, A. (2016). The influence of casein haplotypes on quality, coagulation, and yield traits of milk from Italian Holstein cows. Journal of Dairy Science, 99(5), 3288-3294.

Pilonetto, F., Ladeira, G. C., Salvia, M., Gervásio, C. I., Zampar, A. & Cucco, D. C. (2019). Genetic association between traits of economic interest and identification of haplotypes for genetic diseases in Holstein cows raised in Brazil. Livestock Research Rural Development, 31(188).

Pimentel, E. C. G., Erbe, M., Konig, S. & Simianer, H. (2011). Genome partitioning of genetic variation for milk production and composition traits in Holstein cattle. Frontiers in Genetics, 2(19), 1-11.

Poulsen, N. A., Bertelsen, H. P., Jensen, H. B., Gustavsson, F., Glantz, M., Lindmark Mansson, H., Andrén, H., Paulsson, M., Bendixen, C., Buitenhuis, A. J. & Larsen, L. B. (2013). The occurrence of noncoagulation milk and the association of bovine milk coagulation properties with genetic variants of the caseins in 3 Scandinavian dairy breeds. Journal of Dairy Science, 96(8), 4830-4842.

R Core Team. (2021). R: A language and environment for statistical computing (Version 4.1.2). R Foundation Statistical Computing, Vienna, Austria. https://www.R-project.org/.

Riaz, M. N., Malik, N. A., Nasreen, F., Qureshi, J. A. & Khan, S. U. H. (2012). Genetic Variability in the kappa-casein gene in Sahiwal, Cholistani and Red Sindhi cattle breeds. International Journal of Dairy Technology, 65(2), 208-211.

Visker, M. H. P. W., Dibbits, B. W., Kinders, S. M., Van Valenberg, H. J. F., Van Arendonk, J. A. M., & Bovenhuis, H. (2010). Association of bovine b-casein protein variant I with milk production and milk protein composition. Animal Genetics, 42(2), 212-218.

Downloads

Published

02/07/2022

How to Cite

PILONETTO, F.; COELHO LADEIRA, G.; SALVIAN, M.; ZAMPAR, A.; CUCCO, D. de C. The genotypic profile of milk proteins in Holstein cows raised in South of Brazil. Research, Society and Development, [S. l.], v. 11, n. 9, p. e2911931530, 2022. DOI: 10.33448/rsd-v11i9.31530. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/31530. Acesso em: 25 apr. 2024.

Issue

Section

Agrarian and Biological Sciences