Characterization of Candida spp. isolated from the bloodstream in a reference hospital in Brazil: using MALDI-TOF/MS to identify unusual species of Candida

Authors

DOI:

https://doi.org/10.33448/rsd-v11i11.33243

Keywords:

Candidemia; MALDI-TOF/MS; Antifungal agents; Mortality.

Abstract

Candidemia is a health problem with a high mortality rate. The aims of this study were to compare the identification of Candida spp. via traditional methodologies and mass spectrometry (MALDI-TOF/MS) in order to verify the antifungal susceptibility profile of these isolates and analyze the clinical evolution of patients. This was a two-year prospective cohort conducted at the Hospital de Clínicas de Uberlândia (HCU-UFU). Candida spp. were identified via VITEK®-2 and MALDI-TOF/MS. Antifungal susceptibility tests were conducted via VITEK®-2. Clinical-demographic data were assessed from medical records and variables were described as mean, standard deviation, frequency and percentage, and risk factors were evaluated using univariate and multivariate analysis. A total of 113 patients and 126 Candida spp. isolates were studied. C. albicans was most frequent (30%); MALDI-TOF/MS identified unusual species, including misidentification of C. famata. Most of the isolates (95%) were susceptible to the tested antifungals. Most patients were men (65%) and older than 60 years of age (45%); 85% of patients were treated with fluconazole. The 30-day mortality rate was 56%; patients who did not receive treatment and those who started later had a lower survival rate (p <0.0001). MALDI-TOF/MS was able to identify unusual Candida spp. Candidemia presented a high mortality rate; the correct identification of Candida spp. and the introduction of appropriate and timely therapy are essential for increasing patient survival.   

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15/08/2022

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AGUIAR, P. A. D. F. de; MENEZES, R. de P.; PENATII, M. P. A.; ARAÚJO, L. B. de .; RÖDER, D. V. D. de B. Characterization of Candida spp. isolated from the bloodstream in a reference hospital in Brazil: using MALDI-TOF/MS to identify unusual species of Candida . Research, Society and Development, [S. l.], v. 11, n. 11, p. e69111133243, 2022. DOI: 10.33448/rsd-v11i11.33243. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/33243. Acesso em: 19 apr. 2024.

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Vol. 11 No. 11

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