Homeopathic immunological for dairy calves and its effects on metabolism, immune reactions, serum antioxidant responses, and growth performance
DOI:
https://doi.org/10.33448/rsd-v11i14.35987Keywords:
Dairy cattle; Performance; Immunity.Abstract
This study determined whether adding an immunological homeopathic product (Imunorgan®) to the calf diet during the first months of life improves immune reactions, antioxidant responses, metabolism, and growth. The 12 calves with an average of 36±4 kg and 30 days of life, were divided into a control (n=6) and treatment (n=6) group who received the homeopathic product in milk during the first 30 days and subsequently in the concentrate (providing 10 g/day of the immunological homeopathic, based on Arsenicum album, Baptisia tinctoria, Calcarea carbonica, Iodum purum, Medicago sativa, Mercurius solubilis, Nux vomica, Pyrogenium, and sucrose vehicle). The animals in the treatment group had more significant body weight gain from 1 to 30 days of the experiment (p≤0.05). There was a treatment effect on hematologic results, with hemoglobin concentration showing major treatment x day interaction on day 90 (p≤0.05). Leukocyte, neutrophil, and lymphocyte counts were higher in the treatment group (p≤0.05). The albumin concentration in the treatment group animals was lower than in the control group (p≤0.05). The globulin concentration was higher in the treatment group (p≤0.05). There was a treatment x day interaction for the concentration of protein thiols on days 30, 60, and 90, with higher serum levels in calves in the treatment group (p≤0.05). Serum levels of reactive oxygen species were lower in calves of the treatment group (p≤0.05). The animals of the treated group showed a higher concentration of IgA. During the experiment, levels of ceruloplasmin, haptoglobin, transferrin, acid glycoprotein, and light-chain IgG also changed (treatment x day interaction). These findings suggest that the homeopathic product stimulated immunological responses.
References
Ali, S. F., Lebel, C. P., & Bondy, S. C. (1992) Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicol. 113, 637-648
Banerjee, P., Bhattacharyya, S.S., Pathak, S., Naoual, B., Belon, P., & Khuda-Bukhsh, A.R. (2008) Comparative Efficacy of Two Microdoses of a Potentized Homeopathic Drug, Arsenicum Album, to Ameliorate Toxicity Induced by Repeated Sublethal Injections of Arsenic Trioxide in Mice. Pathobiol. 75, 156–170
Bakema, J. E., & Van Egmond, M. (2011) Immunoglobulin A: A next generation of therapeutic antibodies? mAbs. 3 (4), 352-361. https://doi.org/10.4161/mabs.3.4.16092
Barkema, H.W., Von Keyserlingk, M.A.G., Kastelic, J.P., Lam, T.J.G.M., Luby, C., Roy, J.P., LeBlanc, S.J., Keefe, G.P., & Kelton, D.F. (2015) Invited review: Changes in the dairy industry affecting dairy cattle health and welfare. Int. J. Dairy Sci. 98(11), 7426-7445.
Cermin, A., Berry, C., Burton, S., & Howlett, E. (2019) The effects of the FTC-mandated disclosure on homeopathic product purchase intentions and efficacy perceptions. J. Bus. Res. 101, 47-58.
De Vries, A., & Marcondes, M. (2020) Review: Overview of factors affecting productive lifespan of dairy cows. Anim. 14, 155-164. 10.1017/S1751731119003264
Doehring, C., & Sundrum, A. (2016) Efficacy of homeopathy in livestock according to peer-reviewed publications from 1981 to 2014. Vet Rec. 179(24), 628-628. 10.1136/vr.103779
Downs, J., Wolf, C.E., Williams, G., Cumpston, K.L., Kershner, E., & Wills, B.K. (2021) Negligible Nux Vomica: Homeopathic Nux Vomica remedies don’t contain strychnine? Toxicon. 200, 1–2
Fagliar, J.J, Santana, A.E., Lucas, F.A., Campos, E., & Curi, P.R. (1998) Constituintes sanguíneos de bovinos recém-nascidos das raças Nelore (Bos indicus) e holandesa (Bos taurus) e de bubalinos (Bubalis bubalus) raça Murrah. Arq. Bras. Med. Vet. Zootec. 50, 253-262
Ferrazza, R.A., Moura, G.F.U., & Lopes, M.A. (2018) Characterization of the factors associated with the growth phase of dairy cattle in family farming. Semin. Cienc. Agrar. 39(4), 1653-1667.
Fischer-Tlustos, A.J., Welboren, A.C., Hare, K.S., & Steele, M.A. (2020) New concepts in calf nutrition: the first week of life. WCDS Adv Dairy Technol 32: 149-158
Habig, W.H., Pabst, M.J., & Jakob, W.B. (1974) Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130–7139.
Hurley, W.L., & Theil, P.K. (2011) Perspectives on immunoglobulins in colostrum and milk. Nutrients. 3(4), 442-474. 10.3390/nu3040442.
Kaneco J., Harvey, J., & Bruss, M. (2008) Clinical Biochemistry of Domestic Animals. 6ed, Academic Press. https://doi.org/10.1016/B978-0-12-370491-7.X0001-3
Kerr, M. (2000) Function of immunoglobulin A in immunity. Gut. 47(6), 751-752
Kundu, S.N., Mitra, K., & Khuda-Bukhsh, A.R. (2000) Efficacy of a potentized homoeopathic drug (Arsenicum-Album-30) in reducing cytotoxic effects produced by arsenic trioxide in mice: III. Enzymatic changes and recovery of tissue damage in liver. Complement. Ther. Med. 8(2), 76–81.
Jentzsch, A.M., Bachmann, H., Fürst, P., & Biesalski, H.K. (1996) Improved analysis of malondialdehyde in human body fluids. Free Radic. Biol. Med. 20(2), 251-256.
Jyothilakshmi, V., Thellamudhu, G., Chinta, R., Alok, K., Anil, K., Debadatta, N., & Kalaiselvi, P. (2014) Beneficial antioxidative effect of the homeopathic preparation of Berberis vulgaris in alleviating oxidative stress in experimental urolithiasis. Forsch Komplementmed. 21(1), 7-12
Lees, P., Pelligand, L., Whiting, M., Chambers, D., Toutain, P.L., & Whitehead, M.L. (2017) Comparison of veterinary drugs and veterinary homeopathy: part 2. Vet. Rec. 181(8), 198-207.
Leroy, G., Baumung, R., Boettcher, P., Scherf, B., & Hoffmann, I. (2016) Review: Sustainability of crossbreeding in developing countries; definitely not like crossing a meadow. Animal. 10(2), 262-273. 10.1017/S175173111500213X
Mathie, R.T., & Clausen, J. (2014) Veterinary homeopathy: systematic review of medical conditions studied by randomised placebo‐controlled trials. Vet. Rec.. 175(15), 373-381
Marchiori, M.S., Da Silva A.S., Glombowsky, P., Campigotto, G., Favaretto, J.A., & Jaguezeski, A.M. (2019) Homeopathic product in dog diets modulate blood cell responses. Arch. Vet. Sci. 24(4), 92-101.
Mallick, P., Mallick, J.C., Guha, B., & Khuda-Bukhsh, A.R. (2003) Ameliorating effect of microdoses of a potentized homeopathic drug, Arsenicum Album, on arsenic-induced toxicity in mice. BMC Complement. Altern. Med. 3(1), 1–13
Marklund, S., & Marklund, G. (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47(3), 469-474.
Oliveira, S.M., Oliveira, C.C., Abud, A.P.R., Guimarães, F.S.F., Di Bernardi, R.P., Coletto, E.L.O., & Buchi, D.F. (2011) Mercurius solubilis: actions on macrophages. Homeopath. 100(4), 228–236
Pabst, O., & Slack, E. (2020) IgA and the intestinal microbiota: the importance of being specific. Mucosal Immunol., 13, 12–21
Ring, S.C., McCarthy, J., Kelleher, M.M., Doherty, M.L., & Berry, D.P. (2018) Risk factors associated with animal mortality in pasture-based, seasonal-calving dairy and beef herds. J. Anim. Sci. 96(1), 35-55. 10.1093/jas/skx072.
Sandoval, C.H., Morfin, L.L., & Lopez, B.B. (2018) Preliminary research for testing Baptisia tinctoria 30c effectiveness against salmonellosis in first and second quality broiler chickens. Br. Homeopath. J. 87(3), 131–134
Shahabi, S. (2020) Homeopathy and Immune Boosting: Research Review and Discussion of Possible Mechanism of Action. Am. J. Homeopath. Med. 113(3), 42-46.
Sedlak, J., & Lindsay, R.H. (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal. Biochem.. 25, 192–205.
Şenel, E. (2019) Evolution of homeopathy: A scientometric analysis of global homeopathy literature between 1975 and 2017. Complement. Ther. Clin. Pract. 34, 165-173.
Silva, D.J., & Queiroz, A.C. (2002) Food analysis: chemical and biological methods. Viçosa: UFV.
Upadhaya, S.D., Park, H.J., & Kim, I.H. (2021) Effects of group size and homeopathic product supplement consisting of botanicals and mineral mixture on the performance and production traits of finishing pigs. Can. J. Anim. Sci. 101(3), 548–557.
Van Soest, P.J., Robertson, J.B., & Lewis, B.A. (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74(10), 3583-3597
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