Endophytic fungi associated with the restinga bromeliads the National Park of Jurubatiba Restinga, Rio de Janeiro - Brazil
DOI:
https://doi.org/10.33448/rsd-v9i7.4298Keywords:
Bioprospecting; Biotechnology; Interaction; Mycoparasitism.Abstract
Throughout evolution, plants have adapted to different terrestrial habitats and developed the ability to interact with different species of living things, among which, fungi stand out, and have shown great biotechnological or scientific interest and have been isolated and studied from plants in different natural biomes. Thus, the objective was to identify cultivable endophytic fungi associated with Bromeliaceae from the Jurubatiba restinga, state of Rio de Janeiro. Two collections were made in the Restingas de Jurubatiba National Park (PNRJ), located on the north coast of the state of Rio de Janeiro. Samples of healthy plant leaves for the isolation of endophytic fungi were collected from the species of bromeliads inhabiting this restinga: Aechmea nudicaulis (L.) Griseb. (collection 1) and Bromelia antiacantha Bertol (collection 2). Plants were collected at random in order to cover their most representative range. In the laboratory, under conditions aseptic procedures, an isolation procedure for endophytic fungi kept under storage was carried out and, later, identification of isolated fungi, under an optical microscope. The fungal genera were identified: Aspergillus sp., Bipolaris sp., Curvularia sp., Monilia sp., Nigrospora sp., Penicillium sp., Pestalotiopsis sp., And Trichoderma sp. Isolates of Bipolaris sp., Curvularia sp. and Monilia sp. were identified in both collected bromeliads. Within each genus, the isolates were grouped and characterized into subtypes and, based on a bibliographic survey, the endophytic fungi found could be studied for different biotechnological purposes and applicability in different aspects of knowledge, and this was the first report of endophytic fungi. in these species of bromeliads.
References
Araujo DSD, Pereira MCA & Pimentel MCP (2002). Flora e estrutura de comunidades na restinga de jurabatiba - síntese dos conhecimentos com enfoque especial para a formação aberta de clusia. In: Rocha CFD, Araújo WL, Lima AOS, Azevedo JL, Marcon J, Sobral JK & Lacava PT. Manual de isolamento de microrganismos endofíticos. Piracicaba.
Akello J, Dubois T, Gold CS, Coyne D, Nakavuma J, Paparu P (2007) Beauveria bassiana (balsamo) vuillemin as an endophyte in tissue culture banana (musa spp.). Journal of invertebrate pathology, 96: 34–42. DOI: 10.1016/j.jip.2007.02.004.
Arnold AE, Mejía LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA (2003). Fungal endophytes limit pathogen damage in a tropical tree. Proceedings of the national academy of scienses oh the United States of America. Washington, 100: 15649-15654. DOI: https://doi.org/10.1073/pnas.2533483100.
Alfenas AC, Mafia RG (2007). Métodos em fitopatologia. Viçosa: UFV, 382p
Assunção MMC (2010). Fungos endófitos isolados de folhas de bananeira (musa spp.) e seleção de antagonistas a fitopatógenos dessa cultura. 2010. 172f. Tese (Doutorado em biologia de fungos) - Universidade Federal de Pernambuco, recife,
Azevedo JL, Araújo WL (2007). Endophytic fungi of tropical plants: diversity and biotechnological aspects. In: Ganguli, B.n.; Deshmukh, S. K. Fungi multifaceted microbes. New delhi, Anamaya Publishers .189-207.
Bachmann J, Zierold R, Phil YTC, Hauert R, Phys-Dipl CS, Grund-schmidt R, Rheinlander B, Grundmann M, Gosele U, Nielsch, K (2008). Selbstkatalytische atomlagenabscheidung von siliciumdioxid. Angewandte chemie. 120(33): 6272–6274. DOI: https://doi.org/10.1002/ange.200800245
Bae H, Sicher RC, Kim MS, Kim SH, Strem MD, Melnick RL, Bailey BA (2009). The beneficial endophyte Trichoderma hamatum isolate dis 219b promotes growth and delays the onset of the drought response in Theobroma cacao. Journal of experimental botany, 60 (11): 3279–3295. DOI: 10.1093/jxb/erp165
Bayat F, Mirlohi A, Khodambashi M (2009). Effects of endophytic fungi on some drought tolerance mechanisms of tall fescue in a hydroponics culture. Russian journal of plant physiology, 56(4): 510-516. DOI: 10.1134/S1021443709040104
Baldrian P, Gabriel J (2002). Copper and cadmium increase laccase activity in pleurotus ostreatus, Fems Microbiology Letters, 20(1): 69-74. DOI: 10.1111/j.1574-6968.2002.tb10988.x
Barnett HL, Hunter BB (1998). Illustrated genera of imperfect fungi. 4th ed. Saint Paul, MS: Aps, Press,
Bizukojc M, Ledakowicz S (2004). The kinetics of simultaneous glucose and fructose uptake and product formation by Aspergillus niger in citric acid fermentation. Process biochemistry, 39: 2261-2268. DOI: 10.1016/j.procbio.2003.11.017
Benítez T, Rincón AM, Limón MC & Condón AC (2004). Biocontrol mechanisms of Trichoderma strains. International microbiology, 7(4): 249-260. ISSN 1139-6709
Bennet JW (2010). An overview of the genus aspergillus. Disponível em: < http://open-access-biology.com/aspergillus/aspergillusch1.pdf> acesso em: 22 fev. 2017
Cao R, Liu X, Gao K, Mendgen K, Kang Z, Gao J, Dai Y, Wang X (2009). Mycoparasitism of endophytic fungi isolated from reed on soilborne phytopathogenic fungi and production of cell wall–degrading enzymes in vitro. Current microbiology, 59: 584–592. DOI: 10.1007/s00284-009-9477-9
Chuang CC, Kuo YL, Chao CC & Chao WL (2007). Solubilization of inorganic phosphates and plant growth promotion by Aspergillus niger. Biology and fertility of soils, 43: 575-584. DOI 10.1007/s00374-006-0140-3
Chomcheon P, Wiyakrutta S, Aree T, Sriubolmas N, Ngamrojanavanich N, Mahidol C, Ruchirawat S, Kittakoop P (2010). Curvularides a-e: antifungal hybrid peptide-polyketides from the endophytic fungus Curvularia geniculata. Chem. Eur. J, 16:11178–11185. DOI: 10.1002/chem.201000652.
Contreras-Cornejo HA, Macías-Rodríguez L, Cortés-Penagos CE, López-Bicio J (2009) Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant physiology, 149(3): 1579–1592. DOI: https://doi.org/10.1104/pp.108.130369.
Costa CR (2007). Fungos associados às plantas ornamentais tropicais no distrito federal. 2007. 98f. Dissertação (Mestrado em Fitopatologia) - Universidade de Brasília.
Del-Claro K. (2012). Origens e importância das relações plantas-animais para a ecologia e conservação. In: ______. Torezan-Silingardi HM. (orgs). Ecologia das interações plantas-animais: uma abordagem ecológico-evolutiva. Rio de janeiro: Technical Books, p. 336.
Evidente A, Andolfi A, Cimmino A, Vurro M, Fracchiolla M, Charudattan R, Mot A. (2005). Drazepinone, a trisubstituted tetrahydronaphthofuroazepinone with herbicidal activity produced by drechslera siccans. Phytochemistry, 66(6): 715 – 721. DOI: https://doi.org/10.1016/j.phytochem.2005.02.008
Esteves FA, Scarano FR (orgs.). (2004). Pesquisas de longa duração na restinga de jurabatiba: ecologia, história natural e conservação. São carlos: RIMA, 59-76.
Fonseca LCN, Vizentin-Bugoni J, Rech A, Alves MA (2015). Plant-hummingbird interactions and temporal nectar availability in a Restinga from Brazil. Anais da academia brasileira de ciências, 87(4): 2163-2175. DOI: 10.1590/0001-3765201520140349
Flora do brasil 2020 em construção. Jardim botânico do rio de janeiro. Disponível em: <http://floradobrasil.jbrj.gov.br/ >. Acesso em: 07 mai. 2017
Ferreira LS (2010). Caracterização de isolados de curvularia spp. Endofíticos de milho (zea mays l.) por parâmetros morfológicos e moleculares.118 f. Dissertação (mestre em ciências biológicas) - Universidade Federal do Paraná, Curitiba.
Filho MRC, Mello SCM, Santos RPE, Menêzes JE (2008). Avaliação de isolados de Trichoderma na promoção de crescimento, produção de ácido indolacético in vitro e colonização endofítica de mudas de eucalipto. Boletim de pesquisa e desenvolvimento, 226. Brasília, Embrapa recursos genéticos e biotecnologia.
Freire FCO, Bezerra JL (2001). Foliar endophytic fungi of Ceará state (Brazil): A preliminary study. Summa Phytopathologica, 27(3): 304-08.
Gomes JML, Silva NNF (2013). Bromeliaceae das Restingas do estado do Espírito Santo, Brasil. Natureza online, 11(2): 79-89.
Gallo MBC, Chagas FO, Almeida MO, Macedo CC, Cavalcanti BC, Barros FWA, Moraes MO, Costa-Lotufo LV, Pessoa C, Bastos JK, Pupo MT (2009). Endophytic fungi found in association with smallanthus sonchifolius (asteraceae) as resourceful producers of cytotoxic bioactive natural products. Journal of basic microbiology, 48: 1-10. DOI: 10.1002/jobm.200800093
Gibbons JG, Rokas A. (2013). The function and evolution of the Aspergillus genome. Trends in microbiol., 21: 14-22. DOI: 10.1016/j.tim.2012.09.005
Grigoletti JRA, Figueredo A, García C. (2000). Perspectivas do uso do controle biológico contra doenças florestais. Revista floresta (Brasil) 30:155-165
Hong L, Zou WX, Meng JC, Hu J, Tan RX (2000). New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant science, 151: 67–73. DOI: doi.org/10.1016/S0168-9452(99)00199-5
Hyde KD, Soytong K. (2008). The fungal endophyte dilemma. Fungal diversity, 33: 163-173. DOI: 10.1371/journal.pone.0084539
Hanlin RT (1996). Illustrated genera of ascomycetes. Aps press, 274p.
Hao J, Song F, Huang F, Yang C, Zhang Z, Zheng Y, Tian X. (2007). Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye. Journal of industrial microbiology & biotechnology, 34(3): 233-240. DOI: 10.1007/s10295-006-0191-3
Hoyos-Carvajal L, Orduz SE, Bissett J. (2009). Growth stimulation in bean (Phaseolus vulgaris l.) by Trichoderma. Biological control, 51: 409–416. DOI: doi.org/10.1016/j.biocontrol.2009.07.018
Kruschewsky M.C. (2010). Taxonomia e ecologia do gênero Pestalotiopsis no Brasil, com ênfase para a Mata Atlântica do Sul da Bahia. 59 f. Dissertação (mestre em produção vegetal) - Universidade Estadual de Santa Cruz, Ilhéus.
Kunieda-Alonso S, Alfenas AC & Maffia LA (2005). Sobrevivência de micélio e escleródios de Rhizoctonia solani tratados com Trichoderma spp., em restos de cultura de Eucalyptus sp. Fitopatologia brasileira, 30(2): 164-168. DOI: doi.org/10.1590/S0100-41582005000200010
Linnakoski R, Puhakka H, Pappinen A. (2011). Endophytic fungi isolated from khaya anthotheca in ghana. Fungal ecology. 12(3): 444-453. DOI: doi.org/10.1016/j.funeco.2011.08.006
Lima TEF (2010). Micobiota endofítica de Vitis labrusca l. cv. Isabel em regiões do vale do Siriji, Pernambuco, Brasil. 58f. Dissertação (mestrado) -Universidade Federal de Pernambuco, 2010.
Lins SRO, Coelho RSB (2004). Ocorrência de doenças em plantas ornamentais tropicais no estado de Pernambuco. Fitopatologia brasileira, 29(3): 332-335. DOI: doi.org/10.1590/S0100-41582004000300019
Liu AR, Chen SC, Wu SY, Xu T, Guo LD, Jeewon R, Wei JG. (2010). Cultural studies coupled with DNA based sequence analyses and ITS implication on pigmentation as a phylogenetic marker in Pestalotiopsis taxonomy. Molecular phylogenetics evolution, 57: 528-35. DOI: 10.1016/j.ympev.2010.07.017
Louzada GAS, Carvalho DDC, Mello SCM, Lobo Júnior M, Martins I, Braúna LM. (2009). Potencial antagônico de Trichoderma spp. originários de diferentes ecossistemas contra Sclerotinia sclerotiorum e Fusarium solani. Biota neotropica, 9(3): 145–149. DOI: doi.org/10.1590/S1676-06032009000300014
Magalhães WCS, Missagia RV, Costa FAF, Costa MCM. (2008). Diversidade de fungos endofíticos em candeia Eremanthus erythropappus (dc).macleish. Cerne, lavras, 14(3): 267-273.
Manamgoda DS, Cai L, Mckenzie EHC, Crous PW, Madrid H, Chukeatirote E, Shivas RG, Tan YP, Hyde KD (2012). A phylogenetic and taxonomic re-evaluation of the Bipolaris – Cochliobolus – Curvularia complex. Fungal diversity, 56: 131-144. DOI: doi.org/10.1007/s13225-012-0189-2
Marinho AMR, Marinho PSB, Rodrigues Filho E. (2007). Constituintes químicos de Penicillium sp, um fungo endofítico isolado de Murraya paniculata (rutaceae). Revista ciências exatas e naturais, 9(2): 189-199.
Martins-Corder MP, Melo IS (1998). Antagonismo in vitro de Trichoderma spp. a Verticicillium dahliae kleb. Science agriculture, 55(1). DOI: doi.org/10.1590/S0103-90161998000100002
Menezes LFT, Araujo DSD. (2005). Formações vegetacionais da restinga da marambaia, Rio de Janeiro. In: MENEZES, L. F. T.; PEIXOTO, A. L.; ARAÚJO, D. S. D. (orgs.). História natural da marambaia. Rio de janeiro: edur, 67-120.
Mussi-Dias V, Araújo ACO, Silveira SF, Rocabado JMA, Araújo KL. (2012). Fungos endofíticos associados a plantas medicinais. Revista brasileira de plantas medicinais, 14(2): 261-266. DOI: doi.org/10.1590/S1516-05722012000200002.
Narloch C, Oliveira VL, Anjos JT, Silva Filho GN. (2002). Respostas da cultura do rabanete à inoculação de fungos solubilizadores de fosfatos. Pesquisa agropecuária brasileira, 37(6): 841-845. DOI: doi.org/10.1590/S0100-204X2002000600013
Obuekwe CO, Badrudeen AM, Al-Saleh E, Mulder JL. (2005). Growth and hydrocarbon degradation by three desert fungi under conditions of simultaneous temperature and salt stress. International biodeterioration & biodegradation, 56(4): 197-205. DOI: doi.org/10.1016/j.ibiod.2005.05.005
Oses R, Valenzuela S, Freer J, Sanfuentes E, Rodriguez J. (2008). Fungal endophytes in xylem of healthy chilean trees and their possible role in early wood decay. Fungal diversity, 33: 77- 86. DOI: 10.3389/fmicb.2017.01286
Pereira OJ (2003). Restinga: origem, estrutura e diversidade. In: Jardim, M. A. G.; Bastos, M. N. C.; Santos, J. U. M. (orgs.). Desafios da botânica brasileira no novo milênio: inventário, sistematização e conservação da biodiversidade vegetal. Belem: Sociedade Brasileira De Botânica, 177-179.
Papagianni M, Frank W, Mattey M. (2005). Fate and role of ammonium ions during fermentation of citric acid by Aspergillus niger. Applied and environmental microbiology, 71: 7178-7186. DOI: 10.1128/AEM.71.11.7178-7186.2005
Pereira AS, Shitsuka DM, Parreira FJ & Shitsuka R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Pessoa WRLS, Barguil BM, Oliveira SMA, Coelho RSB. (2008). Ocorrência de Pestalotiopsis palmarum em Caryota mitis. Summa phytopathologica, 34(1): 95, 2008. DOI: doi.org/10.1590/S0100-54052008000100023
Pimentel IC, Figura G, Auer CG. (2010). Fungos endofíticos associados a acículas de Pinus taeda. Summa phytopathologica, 36(1): 85-88. DOI: doi.org/10.1590/S0100-54052010000100016.
Pratt RG (2006). Enhancement of sporulation in species of Bipolaris, Curvularia, Drechslera, and Exserohilum by growth on cellulose-containing substrates. Mycopathologia,162: 133 – 140. DOI: 10.1007/s11046-006-0043-8
Ribeiro TPS. (2009). Fungos queratinofílicos em areia de parques escolares de Boa Vista, Roraima. 47f. Monografia (Pós - graduação em recursos naturais) - Universidade Federal de Roraima. Boa Vista.
Rodrigues AAC, Menezes M. (2002). Detecção de fungos endofíticos em sementes de caupi provenientes de Serra Talhada e de Caruaru, estado de Pernambuco. Fitopatologia brasileira, 27: 532-537. DOI: doi.org/10.1590/S0100-41582002000500016.
Rodrigues RL. (2010). Fungos endofíticos associados à Vellozia compacta mart. ex schult. F. (velloziaceae) presente em afloramentos rochosos nos estados de Minas Gerais e Tocantins. 70 f. Dissertação (mestrado em ecologia de biomas tropicais) - Universidade Federal de Ouro Preto, Ouro Preto.
Rosenblueth M, Martínez-Romero E. (2006). Bacterial endophytes and their interactions with hosts. Molecular plant-microbe interactions 19:827-837. DOI: doi.org/10.1094/MPMI-19-0827
Rodrigues KF, Hesse M, Werner C. (2003). Antimicrobial activities of secondary metabolites produced by endophytic fungi from spondias mombin. Journal basic microbial, 40(4): 261-267. DOI: 10.1002/1521-4028(200008)40:4<261::AID-JOBM261>3.0.CO;2-D
Rizzini CT (1979) tratado de fitogeografia do brasil: aspectos sociológicos e florísticos. 2 ed. São paulo, huncitc, 2 v.
Rakotoniriana EF, Munaut F, Decock C, Randriamampionona D, Andriambololoniaina M, Rakotomalala T, Rakotonirina EJ, Rabemanantsoa C, Cheuk K, Ratsimamanga SU, Mahillon J, El-Jaziri M, Quetin-Leclercq J, Corbisier AM. (2007). Endophytic fungi from leaves of centella asiatica: occurrence and potential interactions within leaves. Antonie van Leeuwenhoek. 93, 27–36. DOI: doi.org/10.1007/s10482-007-9176-0
Redman RS, Sheehan KB, Stout TG, Rodriguez RJ, Henson JM. (2002). Thermotolerance generated by plant/fungal symbiosis. Science, 298. DOI: 10.1126/science.1072191
Santos AS, Santana CVS, Almeida AC, Nascimento ARP, França FS. (2009). Fungos associados a manchas foliares em Heliconia psittacorum cv. Golden torch, no submédio São Francisco. Revista verde, 4(4): 01 – 04.
Sessegolo T, Tochetto C, Zanette RA, Silva AS, Alves SH, Monteiro SG, Santurio JM. (2011). Microbiota fúngica em amostras de água potável e esgoto doméstico. Semina: ciências agrárias, 32(1): 301-306.
Seifert K, Morgan-Jones G, Gams W, Kendrick B. (2011) The Genera of Hyphomycetes. Cbs biodiversity series, v. 9, 997p.
Souza AQL, Souza ADL, Filho AS, Pinheiro MLB, Sarquis MIM, Pereira JO. (2004). Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) Rich e Strychnos cogens Bentham. Acta amazonica, 34(2), 185 -195. DOI: doi.org/10.1590/S0044-59672004000200006
Schuttz R, Araújo LC Sá FS. (2012). Bromélias: abrigos terrestres de vida de água doce na floresta tropical. Natureza online, 10(2): 89-92.
Sologuren FJ, Juliatti FC. (2007). Doenças fúngicas em plantas ornamentais em Uberlândia-MG. Bioscience journal, 23(2): 42-52
Sempere F, Santamarina MP (2007). In vitro biocontrol analysis of Alternaria alternata (fr.) Keissler under different environmental conditions. Mycopathologia, 163: 183- 190. DOI: 10.1007/s11046-007-0101-x
Vassilev N, Medina A, Azcón R & Vassileva M. (2006). Microbial solubilization of rock phosphate on media containing agro-industrial wastes and effect of the resulting products on plant growth and p uptake. Plant and soil, 287: 77-84. DOI: doi.org/10.1007/s11104-006-9054-y
Verzignassi JR, Matos AP, Santos MF, Poltronieri LS, Benchimol RL, Sanches NF (2009). Mancha negra do abacaxi no Pará. Summa phytopathol., 35(1): 76.
Wang FW, Jiao RH, Cheng AB, Tan SH, Song YC (2006) Antimicrobial potential of endophytic fungi residing in Guercus variabilis and brefeldin a obtained from Cladosporium sp. World journal of microbiology and biotechnology, 23: 79- 83. DOI: doi.org/10.1007/s11274-006-9195-4
Watanabe T. (2010). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. Crc press.
Wei JG, Xu T. (2007). Pestalotiopsis kunmingensis, sp. Nov., an endophyte from Podocarpus macrophyllus. Fungal diversity, 15: 247-254.
Yang X, Sears J, Kramer R, Sidhu RS, Hess WM. (2011). Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology, 142: 435-440. DOI: 10.1099/13500872-142-2-435
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