Dicksonia sellowiana Hook. and Nepholepis cordifolia (L.) Lellinger extracts as potential green pesticides: insecticidal activity

Authors

DOI:

https://doi.org/10.33448/rsd-v9i8.5182

Keywords:

Ferns; Insect pests; Bioinsecticides; Plant insecticides; Insecticidal activity.

Abstract

Known as green insecticides, natural plant-based products have become a promising alternative to conventional insecticides. The primary objective of the present study was to analyze, under laboratory conditions, the insecticidal activity of crude ethanol extracts of the fern species Dicksonia sellowiana and Nephrolepis cordifolia against Oncopeltus fasciatus (Hemiptera). Fern leaves were collected from Itatiaia National Park (Brazil), dried and ground using 96% ethanol, with the extract concentrated in a rotary evaporator. The crude extract was used to prepare a 50 mg/mL solution, with acetone as solvent. Qualitative analysis of the terpenoid and phenolic substance profile in the extracts was performed by thin-layer chromatography (TLC). Fourth-instar insects were used, in groups of 10 animals per treatment, with three repetitions. Four treatments were used: D. sellowiana and N. cordifolia extracts, acetone control and water control. Then, 1 μL either of the respective solution was topically applied to the insect abdominal cuticle. After 21 days, all the insects in the water control group had reached adult. The N. cordifolia extract was responsible for 63% (p < 0.0001) of insect mortality around 16 days after treatment, whereas the D. sellowiana exhibited 50% (p < 0.0001) on the 21st day post-treatment. The extracts also caused delays in insect molting and metamorphosis. The D. sellowiana and N. cordifolia extracts exhibited 18% similarity in the terpenoid profile and 0% for phenolic substances. N. cordifolia and D. sellowiana show potential for research on selective biodegradable substances for use as green insecticides.

References

Chipman, A. D. (2017). Oncopeltus fasciatus as an evo-devo research organism. Genesis, 55(5), e23020. https://doi.org/10.1002/dvg.23020

Corrêa, J. C. R., & Salgado, H. R. N. (2011). Atividade inseticida das plantas e aplicações: revisão. Revista Brasileira de Plantas Medicinais, 13(4), 500-506. https://doi.org/10.1590/S1516-05722011000400016

Duprat, R. C., Anholeti, M. C., De Sousa, B. P., Pacheco, J. P. F., Figueiredo, M. R., Kaplan, M. A. C., Santos, M., G., Gonzalez, M., S., Ratcliffe, N., A., Mello, C., B., Paiva, R., P., Feder, D. (2017). Laboratory evaluation of Clusia fluminensis extracts and their isolated compounds against Dysdercus peruvianus and Oncopeltus fasciatus. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy, 27, 59-66.

Feder, D., Gonzalez, M. S., Mello, C. B., Santos, M. G., Rocha, L., Kelecom, A., & Folly, E. (2019). Exploring the Insecticide and Acaricide Potential of Development Regulators obtained from Restinga vegetation from Brazil. Anais da Academia Brasileira de Ciências, 91(1), e20180381. Epub April 08, 2019. https://doi.org/10.1590/0001-3765201920180381

Fürstenberg-Hägg, J., Zagrobelny, M., & Bak, S. (2013). Plant defense against insect herbivores. International journal of molecular sciences, 14(5), 10242–10297. https://doi.org/10.3390/ijms140510242

Fernandes, C. P., Xavier, A., Pacheco, J. P. F., Santos, M. G., Mexas, R., Ratcliffe, N. A., Gonzalez, S., M., Mello, C., B., Rocha, L., & Feder, D. (2013). Laboratory evaluation of the effects of Manilkara subsericea (Mart.) Dubard extracts and triterpenes on the development of Dysdercus peruvianus and Oncopeltus fasciatus. Pest management science, 69(2), 292-301. https://doi.org/10.1002/ps.3388

Ferreira, W. L. B. (1999). Inseticidas de uso domiciliar e controle de vetores de doenças. Insetos e outros invasores de residências, 6, 440-452 Piracicaba: FEALQ.

Fidalgo, O. & Bononi, V. L. R. (1989). Técnicas de coleta, prevenção e herborização de material botânico, 62. São Paulo: Instituto de Botânica.

Gallo, D., Nakano, O., Silveira Neto, S., Carvalho, R. P. L., Baptista, G. C., Berti Filho, E., Parra, J., R., P., Zucchi, R., A., Alves, S., B., Vendramim, J., D., Marchini, L., C., Lopes, J., R., S., & Omoto, C. (2002). Entomologia Agrícola, Biblioteca de Ciências Agrárias, 10(2), 5 - FEALQ, Piracicaba.

Huang, S. Q., Zhang, Z. X., Li, Y. Z., Li, Y. X., & Xu, H. H. (2010). Anti-insect activity of the methanol extracts of fern and gymnosperm. Agricultural Sciences in China, 9(2), 249-256. https://doi.org/10.1016/S1671-2927(09)60090-0

Lewinsohn, T. M., Jorge, L. R., & Prado, P. I. (2012). Biodiversidade e interações entre insetos herbívoros e plantas. DEL CLARO, K.; SILINGARDI, H. M. T. Ecologia das interações plantas-animais: uma abordagem ecológico-evolutiva. Rio de Janeiro, 275-289.

Lima, G. P. (2019). Samambaias e licófitas com atividade inseticida: o estado da arte. Monografia Curso de Ciências Biológicas, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, São Gonçalo.

Lovatto, P., Schiedeck, G., & Mauch, C. (2013). 13917 - Efeito do extrato de Pteridium aquilinum (Dennstaedtiaceae) sobre a preferência alimentar e biologia de Brevicoryne brassicae e Myzus persicae (Hemiptera: Aphididae). Cadernos De Agroecologia, Vol. 8, Nº 2). Recuperado de http://revistas.aba-agroecologia.org.br/index.php/cad/article/view/13917

Oliveira, V.B. (2012). Estudo fitoquímico e atividades biológicas da espécie vegetal Dicksonia sellowiana (Presl.) Hook. (DICKSONIACEAE). Dissertação de Mestrado, Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Paraná, Curitiba.

Koul, O., Walia, S., & Dhaliwal, G. S. (2008). Essential oils as green pesticides: potential and constraints. Biopesticides International, 4(1), 63-84.

Potenza, M. R., Silva, R. D., Artur, V., Felicio, J. D., Rossi, M. H., & Nakaoka Sakita, M. (2004). Avaliação de produtos naturais irradiados para o controle de Blattella germânica (L.)(Dictyoptera: Blattellidae). Arq. Inst. Biol, 71(4), 485-492.

PPG I (2016). A community-derived classification for extant lycophytes and ferns. The Pteridophyte Phylogeny Group. Journal of Systematics and Evolution, 54(6), 563. https://doi.org/10.1111/jse.12229

Quistad, G. B., Staiger, L. E., & Cerf, D. C. (1982). Preparation and biological activity of potential inhibitors of insect juvenile hormone biosynthesis. Journal of Agricultural and Food Chemistry, 30(6), 1151-1154. https://doi.org/10.1021/jf00114a037

Rizzati, V., Briand, O., Guillou, H., & Gamet-Payrastre, L. (2016). Effects of pesticide mixtures in human and animal models: an update of the recent literature. Chemico-biological interactions, 254, 231-246. https://doi.org/10.1016/j.cbi.2016.06.003

Santos, M. G., Kelecom, A., Paiva, S. R., Moraes, M. G., Rocha, L., & Garrett, R. (2010). Phytochemical studies in pteridophytes growing in Brazil: A review. Am. J. Plant Sci. Biotechnology, 4, 113-125.

Tietbohl, L. A., Barbosa, T., Fernandes, C. P., Santos, M. G., Machado, F. P., Santos, K. T., Mello, C., B., Araújo, H., P., Gonzalez, M., S., Feder, D., & Rocha, L. (2014). Laboratory evaluation of the effects of essential oil of Myrciaria floribunda leaves on the development of Dysdercus peruvianus and Oncopeltus fasciatus. Revista Brasileira de Farmacognosia, 24(3), 316-321. https://doi.org/10.1016/j.bjp.2014.07.009

Viegas Júnior, C. (2003). Terpenos com atividade inseticida: uma alternativa para o controle químico de insetos. Química Nova, 26(3), 390-400. https://doi.org/10.1590/S0100-40422003000300017

Xavier, G. S. A., Selvaraj, P., & John, N. (2016). Impact of phytoecdysone fractions of the ferns Cyclosorous interruptus, Christella dentata and Nephrolepis cordifolia on the biology of Spodoptera litura (Fab.). Journal of Biopesticides, 9(2), 125.

Zagrobelny, M., Bak, S., Rasmussen, A. V., Jørgensen, B., Naumann, C. M., & Møller, B. L. (2004). Cyanogenic glucosides and plant–insect interactions. Phytochemistry, 65(3), 293-306. https://doi.org/10.1016/j.phytochem.2003.10.016

Zucchi, R. A., Vendramim, J. D., & Berti Filho, E. (1992). Importância dos insetos e manejo de pragas. In Curso de entomologia aplicada à agricultura, 1-3 Piracicaba: FEALQ.

Downloads

Published

27/06/2020

How to Cite

SOUZA, J. B. de; LIMA, G. P.; FEDER, M. D.; LANCELLOTTI, I. R.; SANTOS, M. G. Dicksonia sellowiana Hook. and Nepholepis cordifolia (L.) Lellinger extracts as potential green pesticides: insecticidal activity. Research, Society and Development, [S. l.], v. 9, n. 8, p. e120985182, 2020. DOI: 10.33448/rsd-v9i8.5182. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/5182. Acesso em: 25 apr. 2024.

Issue

Vol. 9 No. 8

Section

Agrarian and Biological Sciences

License

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.