Genetic variability in populations of Pilosocereus catingicola (Gürke) Byles & Rowley subsp. salvadorensis (Werderm.) Zappi (Cactaceae)
DOI:
https://doi.org/10.33448/rsd-v9i8.5521Keywords:
Facheiro; DNA; Polymorphism.Abstract
Studies on genetic diversity of cacti are important to elucidate evolutionary events and ecological characteristics of populations vulnerable to genetic erosion. The objective of this study was to evaluate the genetic diversity among individuals of Pilosocereus catingicola subsp. salvadorensis occurring in three populations located in the Caatinga do Agreste region of Paraiba, using RAPD markers. For the extraction of DNA, tissues of the parenchyma and cladodium of the stem of the individuals were used by the 2% CTAB method and amplified using 05 primers. The obtained marks were converted into a binary matrix, from which the matrix of genetic dissimilarity was constructed using the arithmetic complement of the Jaccard coefficient and the construction of the dendrogram, by the UPGMA method. All markers tested amplified, being 82.4% polymorphic locus and 10.6% monomorphic for the Arara population; 84.4% polymorphic and 15.6% monomorphic for the Areial population, the Boa Vista population did not show monomorphism, resulting in 100% polymorphism. The averages of heterosigose observed (0.372, 0.492 and 0.135) show that there are genetic divergences within the populations. The primers used were effective in identifying polymorphism in the species. The populations evaluated in this study showed high genetic diversity, both within and between the population, originating fifteen groups among the 120 individuals from the UPGMA hierarchical grouping analysis, and it was possible to discriminate the populations.
References
Casas, A., Otero-Arnaiz, A., Pérez-Negrón, E., & Valiente-Banuet, A. (2007) In situ Management and domestication of plants in Mesoamerica. Annals of Botany, 100(5), 1101–15.
Clark-Tapia, R., Alfonso-Corrado, C., Eguiarte, L. E., & Molina-Freaner, F. (2005) Clonal diversity and distribution in Stenocereus eruca, a narrow endemic cactus of the Sonoran desert. American Journal of Botany, 92(2), 272–8.
CPRM (2005a) - Serviço Geológico do Brasil. Projeto cadastro de fontes de abastecimento por água subterrânea. Diagnóstico do município de Arara, estado da Paraíba. João de Castro Mascarenhas, Breno Augusto Beltrão, Luiz Carlos de Souza Junior, Franklin de Morais, Vanildo Almeida Mendes, Jorge Luiz Fortunato de Miranda (Orgs.) Recife: CPRM/PRODEEM. p.20.
CPRM (2005b) - Serviço Geológico do Brasil. Projeto cadastro de fontes de abastecimento por água subterrânea. Diagnóstico do município de Areial, estado da Paraíba. João de Castro Mascarenhas, Breno Augusto Beltrão, Luiz Carlos de Souza Junior, Franklin de Morais, Vanildo Almeida Mendes, Jorge Luiz Fortunato de Miranda (Orgs.) Recife: CPRM/PRODEEM. p.19.
CPRM (2005c) - Serviço Geológico do Brasil. Projeto cadastro de fontes de abastecimento por água subterrânea. Diagnóstico do município de Boa Vista, estado da Paraíba. João de Castro Mascarenhas, Breno Augusto Beltrão, Luiz Carlos de Souza Junior, Franklin de Morais, Vanildo Almeida Mendes, Jorge Luiz Fortunato de Miranda (Orgs.) Recife: CPRM/PRODEEM. p.11.
Cruz, C. D. (2006) Programa Genes: aplicativo computacional em genética e estatística. Viçosa: Universidade Federal de Viçosa.
Cruz, C. D., Carneiro PCS (2006) Modelos biométricos aplicados ao melhoramento genético. 2ª ed. Viçosa: UFV. p.585.
Faleiro, F. G. (2007) Marcadores genético-moleculares aplicados a programas de conservação e uso de recursos genéticos. Planaltina, DF: Embrapa. Cerrados, 102p.
Fowler, J. A. P., & Glienke, C. (2008) Diversidade genética em populações naturais de vassourão-branco (Piptocarpha angustifolia) por marcador RAPD. Colombo: Embrapa Florestas. Available at: <http://www.infoteca.cnptia.embrapa.br/handle/doc/315640>. Access in: Fev de 2015.
Jaccard, P. (1908) Nouvelles recherches sur la distribution florale. Bulletin de la socieété Vaudoise des Sciences Naturelles, 44, 223–70.
Kruskal, J. B. (1964). Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrika, 29(1),1–27.
Lucena, C. M., Lucena, R. F. P., Costa, G. M., Carvalho, T. K. N., Costa, G. G. S., Alves, R. R. N., Pereira, D. D., Ribeiro, J. E. S., Alves, C. A. B., Quirino, Z. G. M., & Nunes, E. N. (2013) Use and knowledge of Cactaceae in NortheasternBrasil. Journal of Ethnobiology and Ethnomedicine, 9(1),1–11.
Luna-Paez, A., Valadez-Moctezuma, E., Barrientos-Priego, A. F., & Gallegos-Vázquez, C. (2007) Caracterización de Opuntia spp. mediannte semilla con marcadores RAPD e ISSR su possible uso para diferenciación. J. PADC. p.43–59.
Mojema, R. (1977). Hierarquial grouping methods and stopping rules: an evaluation. The Computer Journal, 20(4),359–63.
Pereira, A. S. et al. (2018). Methodology of cientific research. [e-Book]. Santa Maria City. UAB/NTE/UFSM Editors. Accessed on: July, 9th, 2020. Available at: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1
Porebski, S., Bailey, L. G., & Baum, B. R. (1997) Modificatin of a CTAB DNA extraction protocol for plants containing high polysaccaride and polyphenol components. Plant Molecular Biology Reporter, 15(1),8–15.
Rabbani, A. R. C., Silva-Mann, R., & Ferreira, R. A. (2012) Variabilidade genética de Genipa americana L. pertencente ao baixo curso do Rio São Francisco. Revista Árvore, 36(3),401–9.
Rocha, E. A., & Agra, M. F. (2002) Flora do Pico do Jabre, Paraíba, Brasil: Cactaceae Juss. Acta Bot. Bras. 16(1),15–21.
Rohlf, F. J. (1970) Adaptative hierarquical clustering schemes. Systematic Zoology, 19(1),58–82.
Shimizu, J. Y. (2007) Estratégia complementar para conservação de espécies florestais ativas: resgate e conservação de ecótipos ameaçados. Pesquisa Florestal Brasileira, Colombo, (54),7–35.
Sokal, R. R., & Rohlf, F. J. (1962) The comparison of dendrograms by objective methods. Taxonomy, Berlin, 11(2),33–40.
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22),6531–5.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.