Phytochemical screening of extract obtained from the fungus Penicillium purpurogenum from polluted marine environment of Maranhão
DOI:
https://doi.org/10.33448/rsd-v9i8.6109Keywords:
Penicillium; Fitochemistry; Extract.Abstract
This article aims to isolate, identify and screen phytochemistry a prepared extract of the fungus Penicillium purpurogenum, obtained from a polluted marine environment of São Luís-MA. The research was conducted at Laguna da Jansen located in the municipality of São Luís, Maranhão, Brazil. Four sediment collections were performed from May to September 2017, in the morning period. The samples were obtained from a depth of up to 73.5 cm with the aid of a metal dredger. The identification of the fungus followed methodologies of isolation and identification with phytochemical screening of extracts prepared by Matos methodology. Identification confirmed the presence of Penicillium purpurogenum. Phytochemical screening of extracts exhibited the presence of substance phenols, alkaloids, triterpenes, flavanones, flavanols, catechins, flavonoids and steroids, being of interest in the study of biological activities to be performed with the extracts obtained from the fungus under study. Finally, the results obtained in this study ensure a class of chemical constituents, identified by phytochemical screening, which encourage studies of biological activities with the extracts obtained ensuring a potential for applicability of the bioproduct.
References
Bérdy, J. (2012). Thoughts and facts about antibiotics: where we are now and where we are heading. The Journal of antibiotics, 65(8), 385-395.
Bezerra, G. F. D. B., de Almeida, F. C., Neto da Silva, M. A. C., Nascimento, A. C. B., Meireles Guerra, R. N., Viana, G. M. D. C., ... & Brandão Nascimento, M. D. D. S. (2014a). Respiratory allergy to airborne fungi in São Luís–MA: clinical aspects and levels of IgE in a structured asthma program. Journal of Asthma, 51(10), 1028-1034.
Bezerra, G. F. D. B., Gomes, S. M., Silva, M. A. C. N. D., Santos, R. M. D., Muniz Filho, W. E., Viana, G. M. D. C., & Nascimento, M. D. D. S. B. (2014b). Diversity and dynamics of airborne fungi in São Luis, State of Maranhão, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 47(1), 69-73.
Bezerra, G. F. B, da Silva, M. A. C. N., dos Santos, R. M., Haidar, D. M. C., Muniz Filho, W. E., Rosa, I. G., ... & Nascimento, M. D. D. S. B. (2014c). Avaliação da resposta IgE para o entendimento do papel de fungos do ar na alergia respiratória em crianças. Arquivos de Asma, Alergia e Imunologia, 2(3), 119-124.
Bills, G. F., & Gloer, J. B. (2017). Biologically active secondary metabolites from the fungi. The Fungal Kingdom, 1087-1119.
Brae, S., Endnas, A., Keck, J., & Nising, C. F. (2009). Chemistry and biology of mycotoxins and related fungal metabo] ites. Chem Rev, 109, 3903-3990.
Dhake, A. B., & Patil, M. B. (2005). Production of ß-Glucosidase by Penicillium purpurogenum. Brazilian Journal of Microbiology, 36(2), 170-176.
Federation, W. E., & American Public Health Association. (2005). Standard methods for the examination of water and wastewater. American Public Health Association (APHA): Washington, DC, USA.
Ferreira F. S. (2013). Uso de índices de qualidade da água como subsídios na sustentabilidade da Laguna da Jansen.
Fischer, G., & Dott, W. (2002). Quality assurance and good laboratory practice in the mycological laboratory–compilation of basic techniques for the identification of fungi. International journal of hygiene and environmental health, 205(6), 433-442.
Matos, F. D. A. (1997). Introdução à fitoquímica experimental. edições UFC.
Nett, M., Ikeda, H., & Moore, B. S. (2009). Genomic basis for natural product biosynthetic diversity in the actinomycetes. Natural product reports, 26(11), 1362-1384.
Pavan, M. E., Pavan, E. E., López, N. I., Levin, L., & Pettinari, M. J. (2015). Living in an extremely polluted environment: clues from the genome of melanin-producing Aeromonas salmonicida subsp. pectinolytica 34melT. Applied and environmental microbiology, 81(15), 5235-5248.
Riddell, R. W. (1950). Permanent stained mycological preparations obtained by slide culture. Mycologia, 42(2), 265-270.
Shalom, G., Pratten, J., Wilson, M., & Nair, S. P. (2008). Cloning, heterologous gene expression and biochemical characterization of the α-1, 3-glucanase from the filamentous fungus Penicillium purpurogenum. Protein expression and purification, 60(2), 170-175.
Shin, J. H., Sung, J. H., Park, S. J., Kim, J. A., Lee, J. H., Lee, D. Y., ... & Yang, J. M. (2003). Species identification and strain differentiation of dermatophyte fungi using polymerase chain reaction amplification and restriction enzyme analysis. Journal of the American Academy of Dermatology, 48(6), 857-865.
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Copyright (c) 2020 Amanda Mara Teles, Gustavo Oliveira Everton, Adenilde Nascimento Mouchrek, Carla Junqueira Moragas Tellis, Geusa Felipa de Barros Bezerra, Fernando Almeida Souza, Ana Paula Silva de Azevedo-Santos, Maria do Desterro Soares Brandão Nascimento
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