Inhibitory effect of phytochemicals on Candida albicans biofilms on denture liners surfaces
DOI:
https://doi.org/10.33448/rsd-v9i10.8587Keywords:
Denture cleansers; Antifungal agents; Stomatitis.Abstract
Objective: To evaluate in vitro antimicrobial activity of a-terpineol, cinnamaldehyde, 1% sodium hypochlorite, 0,12% chlorhexidine, and corega® in biofilm of Candida albicans (ATCC 90028) developed on soft prosthetic resurfacing surfaces. Methodology: Specimens from the Bosworth Trusoft (Bosworth Company- USA) material were made according to the manufacturer's instructions and the formation of the artificial salivary film was induced for 60 minutes at 37 ° C. The biofilm was seeded (1x106 CFU/mL) and, after initial adhesion (24h, 37°C), exposed to a-terpineol (10 mg/mL), cinnamaldehyde (10 mg/mL), 1% sodium hypochlorite, chlorhexidine 0,12%, corega and saline (growth control). The specimens remained for 1 or 10 minutes, in each solution, with exposures made 24h, 48h and 72h after sowing the biofilm. Metabolic activity was evaluated by the MTT test and Shore A hardness, using a microdurometer. The statistical analysis was performed using the Kruskal-Wallis/ Mann Whitney tests and the t test (α = 5%). Results: It was observed that for exposure for 1 min, cinnamaldehyde, chlorhexidine and hypochlorite were efficient (p> 0,05). At 10 minutes, a-terpineol showed activity similar to growth control (p> 0,05), differing from other substances. Regarding hardness, there was no difference between groups, only for sodium hypochlorite and corega. Conclusion: Cinnamaldehyde, chlorhexidine and hypochlorite demonstrated antifungal activity on Candida albicans biofilm on prosthetic resurfacing surface.
References
AlSheikh, H. M. A., Sultan, I., Kumar, V., Rather, I. A., Al-Sheikh, H., Jan, A. T., & Haq, Q. M. R. (2020). Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance. Antibiotics, 9(8), E480. doi: 10.3390/antibiotics9080480
Altinci, P., Mutluay, M., Soderling, E., & Tezvergil-Mutluay, A. (2018). Antimicrobial efficacy and mechanical properties of BAC-modified hard and soft denture liners. Odontology, 106(1), 83-89. doi: 10.1007/s10266-017-0303-8.
Farzin, M., Bahrani, F., & Adelpour, E. (2013). Comparison of the Effect of two Denture Cleansers on Tensile bond Strength of a Denture Liner. Journal of dentistry (Shiraz, Iran), 14(3), 130–135. PMID: 24724134
Heidrich, D., Fortes, C. B. B., Mallmann, A. T., Vargas, C. M., Arndt, P. B., & Scroferneker, M. L. (2019). Rosemary, Castor Oils, and Propolis Extract: Activity Against Candida albicans and alterations on properties of dental acrylic resins. J Prosthodont, 28(2), e863-e868. doi: 10.1111/jopr.12746.
Kumar, S. M., Kumar, V. A., Natarajan, P., & Sreenivasan, G. (2018). Antifungal Efficacy and the Mechanical Properties of Soft Liners against Candida albicans after the Incorporation of Garlic and Neem: An In vitro Study. Journal of International Society of Preventive & Community Dentistry, 8(3), 212–217. doi: 10.4103/jispcd.JISPCD_343_17.
Lee, H. L., Wang, R. S., Hsu, Y. C., Chuang, C. C., Chan, H. R., Chiu, H. C., Wang, Y. B., Chen, K. Y., & Fu, E. (2018). Antifungal effect of tissue conditioners containing poly(acryloyloxyethyltrimethyl ammonium chloride)-grafted chitosan on Candida albicans growth in vitro. Journal of Dental Sciences, 13(2), 160-166. https://doi.org/10.1016/j.jds.2017.06.004
Li, L., Shi, C., Yin, Z., Jia, R., Peng, L., Kang, S., & Li, Z. (2015). Antibacterial activity of α-terpineol may induce morphostructural alterations in Escherichia coli. Brazilian journal of microbiology, 45(4), 1409–1413. doi: 10.1590/s1517-83822014000400035
Mahboub, F., Salehsaber, F., Parnia, F., Gharekhani, V., Kananizadeh, Y., & Taghizadeh, M. (2017). Effect of denture cleansing agents on tensile and shear bond strengths of soft liners to acrylic denture base. Journal of dental research, dental clinics, dental prospects, 11(3), 183–188. doi: 10.15171/joddd.2017.033
Mancuso, D. N., Goiato, M. C., Zuccolotti, B. C. R., Moreno, A., & Santos, D. M. (2009). Evaluation of Hardness and Colour Change of Soft Liners After Accelerated Ageing. Prim Dent Care, 16(3), 127-30. doi: 10.1308/135576109788634232.
Martorano-Fernandes, L., Cavalcanti, Y. W., & Almeida L. F.D. (2020). Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces. BMC Complement Med Ther, 20(104). https://doi.org/10.1186/s12906-020-02893-9
Nikawa, H., Jin, C., Makihira, S., Egusa, H., Hamada, T., & Kumagai, H. (2003). Biofilm formation of Candida albicans on the surfaces of deteriorated soft denture lining materials caused by denture cleansers in vitro. Journal of oral rehabilitation, 30(3), 243–250. https://doi.org/10.1046/j.1365-2842.2003.01024.x
Pero, A. C., Scavassin, P. M., Leite, A. R. P., Marin, D. O. M., Paleari, A. G., & Compagnoni, M. A. (2013). Effect of immersion cleansers on the bond strength between a denture base resin and acrylic resin teeth. International Journal of Adhesion & Adhesives, 44, 180-183. https://doi.org/10.1016/j.ijadhadh.2013.02.011
Thein, Z. M., Samaranayake, Y. H., & Samaranayake, L. P. (2007). Characteristics of dual species Candida biofilms on denture acrylic surfaces. Archives of oral biology, 52(12), 1200–1208. doi: 10.1016/j.archoralbio.2007.06.007.
Tonon, C. C., Francisconi, R. S., Bordini, E., Huacho, P., Sardi, J., & Spolidorio, D. (2018). Interactions between Terpinen-4-ol and Nystatin on biofilm of Candida albicans and Candida tropicalis. Brazilian dental journal, 29(4), 359–367. https://doi.org/10.1590/0103-6440201802073
Vankadara, S. K., Hallikerimath, R. B., Patil, V., Bhat, K., & Doddamani, M. H. (2017). Effect of Melaleuca alternifolia Mixed with Tissue Conditioners in Varying Doses on Colonization and Inhibition of Candida albicans: An In Vitro Study. Contemporary clinical dentistry, 8(3), 446–450. doi: 10.4103/ccd.ccd_542_17.
Pedroso, R., Balbino, B. L., Andrade, G., Dias, M., Alvarenga, T. A., Pedroso, R., Pimenta, L. P., Lucarini, R., Pauletti, P. M., Januário, A. H., Carvalho, M., Miranda, M., & Pires, R. H. (2019). In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products. Plants (Basel, Switzerland), 8(11), 494. doi: 10.3390/plants8110494
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