Biotechnology of biomass production in vitro of fungi isolated from Pinus

Authors

DOI:

https://doi.org/10.33448/rsd-v9i10.9080

Keywords:

Botrytis cinerea ; Rhizoctonia sp.; Suillus sp.; Biotechnological optimization.

Abstract

Fungi are organisms capable of synthesizing metabolites of industrial interest and the standardization of biomass production for the extraction of these compounds has biotechnological applications. The objective of this work was to optimize the in vitro cultivation process for fungi isolated from Pinus sp., standardizing the best conditions for the production of biomass, contributing to its large scale production. Therefore, the conditions of in vitro cultivation of the fungi Botrytis cinerea, Rhizoctonia sp. and Suillus sp., were evaluated based on the maximum production of dry biomass (PBS), varying temperature, medium and cultivation time. The fungi were grown in glass flasks with liquid culture media, in a BOD chamber, without mechanical stirring. Potato-dextrose broth - PD broth (PD), Czapek - CZ broth (CZ) and Malt Extract - EM broth (EM) were evaluated at temperatures ranging from 8 to 32 ºC and incubation times from 7 to 35 days. PD broth showed better results for fungi B.cinerea and Rhizoctonia sp., when compared to CZ and EM broths, in PBS, while Suillus sp. showed better development in EM broth. The best growth temperature based on PBS was 12 ºC and 16 ºC, with 28 and 35 days of cultivation.

References

Abuqamar, S., Moustafa, K., Tran, L.S. (2017). Mechanisms and strategies of plant defense against Botrytis cinerea. Critical Reviews in Biotechnology, 37(2), 262-274. doi:10.1080/07388551.2016.1271767.

Agrios, G. N. (2005). (5a ed.) Plant pathology, Elsevier (952).

Auer, C. G., Santos dos, A. F. (2016). Doenças dos pinheiros. In: Amorim, L., Rezende, J. A. M, Bergamin, A. F., Camargo, L. E. A (Org.) (5 Ed.), Manual de Fitopatologia (595-602). Ouro Fino: Editora Agronômica Ceres Ltda.

Bérdy, J. (2005). Bioactive microbial metabolites. Journal of Antibiotics, 58(1), 1-26.

Brasil. (2018). Produção da Extração Vegetal e da Silvicultura - PEVS. Instituto Brasileiro de Geografia e Estatística – IBGE. Recuperado de https://www.ibge.gov .br/estatisticas/economicas/agricultura-e-pecuaria/9105-producao-da-extracao-vegetal-e-da-silvicultura.html?=&t=sobre.

Climate-Data.ORG. (2020). https://pt.climate-data.org/america-do-sul/brasil.

Fernández-Varela, R., Ratola, N., Alves, A., Amigo, J.M. (2015). Relationship between levels of polycyclic aromatic hydrocarbons in pine needles and socio-geographic parameters. Journal of Environmental Management, 156(1): 52-61. DOI: https://doi.org/10.1016/j.jenvman.2015.03.019.

Hasan, S., Ansari, M. I., Ahmad, A., Mishra, M. (2015). Major bioactive metabolites from marine fungi: a review. Bioinformation, 11(4), 176–181. DOI: https://dx.doi.org/10.6026%2F97320630011176.

Hatvani, N. (2001). Antibacterial effect of the culture fluid of Lentinula edodes mycelium grown in submerged liquid culture. International Journal of Antimicrobial Agents, 17(1), 71-74.

Jang, J. Y, Subburaj, S., Lee, G. J., Kim, H. S. In vitro screening for Botrytis leaf blight resistance in Lilium species. (2018). Scientia Horticulturae, 239, 133-140. https://doi.org/10.1016/j.scienta.2018.05.009.

Lahlali, R., Serrhini, M. N., Friel, D., Jijakli, M. H. (2007). Predictive modelling of temperature and water activity (solutes) on the in vitro radial growth of Botrytis cinerea Pers. International Journal of Food Microbiology, 114(1), 1-9.

Ligon, B. L. Penicillin: its discovery and early development. (2004). Seminars in pediatric infectious diseases, 15(1), 52-57.

Lopes, F. C. (2011). Produção e análise de metabólitos secundários de fungos filamentosos. (Dissertação de mestrado). Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil.

Montini, R. M. C., Passo, J. R. S., Eira, A. F. (2006). Digital monitoring of mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) on agar medium. Brazilian Journal of Microbiology, 37(1): 90-95. DOI: https://doi.org/10.1590/S1517-83822006000100017.

Moreira, F. M. S., Siqueira, J. O. (2006). (2 Ed.), Microbiologia e bioquímica do solo, (729) UFLA, Lavras.

Mycobank. (2020). Mycobank database. http://www.mycobank.org/defaultinfo.aspx?Pag e=Home.

Papagianni, M. (2004). Fungal morphology and metabolite production in submerged mycelial processes. Biotechnology Advances, 22(3), 189-259. DOI: https://doi.org/10.1016/j.b iotechadv.2003.09.005.

Peres, A. P., Silva-Mann, R., Vieira, M. G. G. C., Machado, J. C. (2003). Variabilidade morfocultural e genética de fungos associados à podridão peduncular do mamão. Ciência e Agrotecnologia, 2(5), 1053-1062.

Pinto, A. C., Silva, D. H. S., Bolzani, V. S., Lopes, N. P., Epifanio, R. A. (2002). Produtos naturais: atualidade, desafios e perspectivas. Química Nova, 25(Supl. 1), 45-61.

Queiroz, F. M., Batista, U. G., Brommnschenkel, S. H. (2004). Avaliação de meios de cultura no crescimento micelial e esporulação de Alternaria brasiliensis. Fitopatologia Brasileira, 29(5), 541-543.

Querellou, J., Cadoret, J. P., Allen, M. J., Collén, J. Marine Biotechnology. (2010). Introduction to Marine Genomics. In: Cock J., Tessmar-Raible, K., Boyen, C., Viard, F. (Ed), Advances in Marine Genomics. Springer, Dordrecht.

Rech, K. S, Oliveira, C. F., Moura, P. F., Oliveira, C. S. P., Hirota, B. C. K., Oliveira, M., Rudiger, A. L., Sá, E. L., Miguel, O. G., Auer, C. G., Miguel, M. D. (2020). Optimisation of Bjerkandera adusta culture conditions for the production of α-α-trehalose. Natural Product Research, 1-5. DOI: https://doi.org/10.1080/14786419.2020.1758092.

Ritchie, F., Bain, R. A., Mcquilken, M. P. (2009). Effects of nutrient status, temperature and pH on mycelial growth, sclerotial production and germination of Rhizoctonia solani from potato. Journal of Plant Pathology, 589-596.

Rossi, M. J., Furigo, A. J., Oliveira, V. L. (2007). Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations. Food Technology & Biotechnology, 45(3): 277-286.

Sánchez, F., Honrubia, M., Torres, P. (2001). Effects of pH, water stress and temperature on in vitro cultures of ectomycorrhizal fungi from Mediterranean forests. Cryptogamie Mycologie, 22(4), 243-258.

Sarwar, S., Khalid, A. N. (2014). Diversity and phylogeny of Suillus (Suillaceae; Boletales; Basidiomycota) from coniferous forests of Pakistan. International Journal of Agriculture & Biology, 16(3), 489-497.

Silva, M. L. C., Martinez, P. F., Izely, N. L., Silva, I. R., Vasconcelos, A. F. D., Cardoso, M. S., Stelutti, R. M., Giese, E. C., Barbosa, A. M. (2006). Caracterização química de glucanas fúngicas e suas aplicações biotecnológicas. Química Nova, 29(1): 85-92.

Silva, F. B., Mazarotto, E. D., Santos, A. F., Auer, C. G. (2018). Caracterização morfológica, fisiológica e patogênica de isolados de Armillaria sp. da região Sul do Brasil. Summa Phytopathologica, 44(1), 23-31. DOI: https://doi.org/10.1590/0100-5405/175653.

Shimizu, J. Y. (2008). (Ed.). Pinus na silvicultura brasileira. Embrapa Florestas, (223). Colombo, Paraná.

Verma, B., Reddy, M. S. (2015). Suillus indicus sp. nov. (Boletales, Basidiomycota), a new boletoid fungus from northwestern Himalayas. India. Mycology, 6(1), 35–41.

Wu, Z. M., Yang, Y., Li, K. T. (2019). Antagonistic activity of a novel antifungalmycin N2 from Streptomyces sp. N2 and its biocontrol efficacy against Rhizoctonia solani. FEMS - Microbiology Letters, 366(3), 1-8. DOI: https://doi.org/10.1093/femsle/fnz018.

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Published

18/10/2020

How to Cite

MOURA, P. F.; AUER, C. G. .; RECH, K. S.; OLIVEIRA, C. F. de .; OLIVEIRA, C. da S. P. de .; HIROTA, B. C. K. .; DIAS, J. de F. G. .; MIGUEL, O. G. .; MIGUEL, M. D. Biotechnology of biomass production in vitro of fungi isolated from Pinus . Research, Society and Development, [S. l.], v. 9, n. 10, p. e7809109080, 2020. DOI: 10.33448/rsd-v9i10.9080. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/9080. Acesso em: 19 apr. 2024.

Issue

Vol. 9 No. 10

Section

Agrarian and Biological Sciences

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Copyright (c) 2020 Paula Francislaine Moura; Celso Garcia Auer; Katlin Suellen Rech; Camila Freitas de Oliveira; Cristiane da Silva Paula de Oliveira; Beatriz Cristina Konopatzki Hirota; Josiane de Fátima Gaspari Dias; Obdúlio Gomes Miguel; Marilis Dallarmi Miguel

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