Influence of the storage conditions of sucupira´s seeds alcoolatures (Pterodon emarginatus) in the presence of their bioactive metabolites
DOI:
https://doi.org/10.33448/rsd-v9i11.9833Keywords:
Sucupira seed; GC-MS; Bioactive metabolites; Macetations.Abstract
The treatment of inflammatory conditions using herbal medicines prepared by macerating sucupira seeds in alcoholic beverages is a common practice. The volatility and lability of the metabolites can promote variations in the chemical composition of herbal medicines, according to the storage conditions. This work aimed to investigate how storage affects the chemical profile of hydroalcoholic extracts of sucupira seeds, mainly in relation to the presence and relative concentration of bioactive metabolites. For this purpose, macerations of sucupira seeds 10% (w/v) in alcohol 70 ° GL and in duplicate were prepared. The packaging of these was carried out in transparent and amber bottles that were stored in different conditions: in direct contact with solar radiation, at room temperature, in a refrigerator and freezer. After 15, 45, 90 and 155 days, aliquots were removed from each sample and these had their chemical profiles determined by gas chromatography technique coupled to mass spectrometry (CG-EM). The storage for periods of 15, 45 and 90 days led to a significant chemical change, only of the samples exposed simultaneously to heat and sunlight. At 115 days of storage, the samples stored in an amber flask, in the other storage conditions, did not exhibit all the extracted metabolites, but maintained the presence of compounds that have anti-inflammatory action. For these results it is possible to conclude that the homemade preparations, stored at room temperature, under refrigeration and frozen, will maintain the presence of the metabolites of anti-inflammatory action even when stored for 115 days, regardless if this storage occurs in an amber or transparent bottle.
References
Adams, R.P. (2007). Identification of essential oil components by gás cromatography/mass spectroscopy, 4th Edition. Allured Publ. Corp, Carol Stream.
Andersen, T. B., Cozzi, F., & Simonsen, H. T. (2015). Optimization of biochemical screening methods for volatile and unstable sesquiterpenoids using HS-SPME-GC-MS. Chromatography, 2, 277-292. doi: 10.3390/chromatography2020277.
Anvisa, Agência Nacional de Vigilância Sanitária (2014). Resolução da diretoria colegiada – RDC n° 26, de 13 de maio de 2014. Diretoria Colegiada da Agência Nacional de Vigilância Sanitária. Brasília, Brasil.
Brito, K. S., Barreto, R. A. P., & Lyra, M. A. M. (2016). Estudo d estabilidade de acompanhamento do fitoterápico sanativo. Revista Eletrônica Estácio Recife, 1 (1).
Fernandes, E. S., Passos, G. F., Medeiros, R., da Cunha, F. M., Ferreira, J., Campos, M. M., Pianowsk, L. F., & Calixto, J. B. (2007). Anti-inflammatory effects of compounds alpha-humulene and (−)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. European Journal of Pharmacology, 569, 228–236. doi: 10.1016/j.ejphar.2007.04.059.
Ferreira, R. A., Viera, M. G. G. C., Pinho, E. V. R. V., & Tonetti, O. A. O. (2001). Morfologia da semente e de plântulas e avaliação da viabilidade da semente de sucupira branca pelo teste de tetrazólio. Revista Brasileira de Sementes, 23(1), 108-115. doi: 10.17801/0101-3122/rbs.v23n1p108-115.
Francomano, F., Caruso, A., Barbossa, A., Fazio, A., La Torre C., Ceramella, J., Mallamaci, R., Saturno, C., Iacopetta, D., & Sinicropi, M. S. (2019). β-Caryophyllene: A Sesquiterpene with Countless Biological Properties. Applied Sciences, 9(24), 5420-5438. doi: 10.3390/app9245420.
Hoscheid, J., & Cardoso, M. L. C. (2015). Sucupira as a potential plant for arthritis treatment and other diseases. Arthritis, 2015, 1-12. doi: 10.1155/2015/379459.
Lee, R. X., Li, Q. Q., & Reed, E. (2012). β-Elemene effectively suppresses the growth and survival of both platinum-sensitive and -resistant ovarian tumor cells. Anticancer Research, 32(8), 3101-3113. PMID: 22843880.
López, P. A., Cirio, A. T., Galindo, R. V. M., Aranda, R. S., & Torres, N. W. (2011). Activity against Streptococcus pneumoniae of the essential oil and δ-cadinene isolated from Schinus mole Fruit. Journal of Essential Oil Research, 23(5), 25–28. doi: 10.1080/10412905.2011.9700477.
Machado, M. S. L., Bruno, K. A., Melo, M. O., & Koike, M. K. (2018). Fitoterapia brasileira: análise dos efeitos biológicos da sucupira (Bowdichia virgilioides e Pterodon emarginatus). Brazilian Journal of Natural Sciences, 2(1), 15-21. doi: 10.31415/bjns.v1i2.10.
Mendes, V. S., Sant’Anna, J. B., Oliveira, S. C. C., Maldonade, I. R., & Machado, E. R. (2017). Inhibitory effects of Pterodon emarginatus bean oil and extract on Staphylococcus aureus. Pharmacognosy and Natural Products. 9(4), 348-353. doi: 10.4103/pr.pr_13_17.
Montanari, R. M., Barbosa, L. C. A., Demuner, A. J., Silva, C. J., Carvalho, L. S., & Andrade, N. J. (2011). Chemical composition and antibacterial activity of essential oils from verbenaceae species: alternative sources of (E)-caryophyllene and germacrene-D. Química Nova, 34(9), 1550-1555. doi: 10.1590/S0100-40422011000900013.
Nascimento, K., Moreira, F., Santos, J., Kassuva, C., Croda, J., Cardoso, C., Viera, M., Ruiz, A., Foglio, M., Carvalho, J., & Formagio, A. (2018) Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol. Journal of Ethnopharmacology, 210, 351-358. doi: 10.1016/j.jep.2017.08.030.
Oliveira, P. F., Alves, J. M., Damasceno, J. L., Oliveira, R. A. M., Dias, H. J., Crotti, A. E. M., & Tavares, D. C. (2015). Cytotoxicity screening of essential oils in cancer cell lines. Revista Brasileira de Farmacognosia, 25(2), 183-188. doi: 10.1016/j.bjp.2015.02.009.
Oliveira, J. B. de., Machado, A. M. de R.., Nelson, D. L.., & Lucas, E. M. F. (2020). Extraction of bioactive metabolites from sucupira seeds (Pterodon emarginatus) using cachaça. Research, Society and Development, 9(11). doi:10.33448/rsd-v9i11.9795
Passos, M. M. B., Albino, R. C., Silva, M. F., & Oliveira, D. R. (2018). A disseminação cultural das garrafadas no Brasil: um paralelo entre medicina popular e legislação sanitária. Saúde Debate. 42(116). doi: 10.1590/0103-1104201811620.
Pereira, R. J., & Cardoso, M. G. (2012). Metabólito secundários vegetais e benefícios antioxidantes. Journal of Biotechonology and Biodiversity, 3(4), 146-152. ISSN: 2179-4804.
Pereira, H. V., Reis, F. A. G.., Machado, A. M. de R., Marques, A. R., Garcia, C. F., Gomes, F. de C. O., & Nelson, D. L. (2020). Characterization of the volatile compounds and anatomical features in commercial samples of Echinodorus plant species. Research, Society and Development, 9(10). doi: 10.33448/rsd-v9i10.9199
Pirani, A. C., Guidi, A. C., Romanichen, F. M. D. F., Ortiz, M. A. L., Teston, A.P. M., Mello, J. C. P., & Araújo, D. C. M. (2020). Estudo de estabilidade acelerada de xarope fitoterápico. Brazilian Journal of Development, 6(9), 69918-69930. doi: 10.34117/bjdv6n9.
Rauter, A. P., Branco, I., Bermejo, J., Gonzáles, A. G., Garcia-Crávalos, M. D., & Feliciano, A. S. (2011) Bioactive humulene derivatives from Asteriscus vogelii. Phytochemistry, 56, 167-171. doi: 10.1016/s0031-9422(00)00304-6.
Russo E. B., & Marcu, J. (2017). Cannabis Pharmacology: The usual suspects and a few promising leads. Advances in Pharmacology, 80, 67-134. doi: 10.1016/bs.apha.2017.03.004.
Santos, J. S., & Almeida, C. C. O. F. Das plantas medicinais à fitoterapia: uma ciência em expansão (2016). Brasília: Editora IFB, 1ª Ed., 214p.
Shamsizadeh, A., Roohbakhsh, A., Ayoobi, F., & Moghaddamahmadi, A. (2017). The role of natural products in the prevention and treatment of multiple sclerosis. In Watson, R., & Killgore, W. D. S. (eds.), Nutrition and Lifestyle in Neurological Autoimmune Diseases (pp. 249-260). Academic Press/Elsevier:Cambridge, MA, USA. ISBN 978-2400212-805298-805293.
Simões, C. M. (2007). Farmacognosia da planta ao medicamento. Florianópolis: Editora da UFSC, 6ª Ed., 1104p. ISBN: 9788570259271.
Souza, C. M. P., Brandão, D. O., Silva, M. S. P., Palmeiras, A. C., Simões, M. O. S., & Medeiros, A. C. D. (2013). Utilização de plantas medicinais com atividade antimicrobiana por usuários do serviço público de saúde em Campina Grande - Paraíba. Revista Brasileira de Plantas Medicinais, 15(2), 188-193. doi: 10.1590/S1516-05722013000200004.
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