Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.

Authors

DOI:

https://doi.org/10.33448/rsd-v10i5.14950

Keywords:

Plant extracts; Bacteria; Susceptibility test.

Abstract

Bacterial resistance to antibiotics has become a serious public health problem worldwide, affecting thousands of people. Thus, there is a need to seek alternative ways to combat them. Therefore, this research aimed to verify the antimicrobial effect of the extract of Annona muricata L. against ATCC strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. The material was collected and the extracts were obtained, then the bacterial strains were prepared for susceptibility testing. The tests proceeded in three stages: crude extracts, chemically fractionated extracts and tests with serial dilutions. Soursop antibacterial activity was observed against all strains tested, the most significant result being the crude extract of the fruit against the strain of E. coli with a halo equal to 22mm. In relation to the tests with the extracts already purified, the fractions from the fruit have more relevant action. The Hexane fraction corresponded to the most expressive results, obtaining halos equal to 28mm, 26mm and 37mm against the strains of E. coli, K. pneumoniae, and S. aureus respectively. As for the tests related to serial dilutions, we observed that after the 1/10 dilution, the extracts no longer had any inhibitory activity on the bacteria under study. However, these results demonstrate the potential of the soursop extract, especially the fruit, as a source of antibacterial compounds. However, it is worthwhile to continue more advanced research with Annona muricata.

References

Alcaráz, L. E, Blanco, S. E., Puig, O. N., Tomás, F., & Ferreti, F. H. (2000). Antibacterial Activity of Flavonoids Against Methicilin – resistant Staphylococcus aureus strains. J. theor. Biol. 205, 231 – 240.

Ali, R., Houghton, P., Raman, A., & Hoult, J. (1998). Antimicrobial and anti-inflammatory activities of extracts and constituents of Oroxylum indicum. Phytomedicine, 5, 375- 81.

Alfaia, D., & Almeida, S. (2016). Avaliação fitoquímica, análise citotóxica e antimicrobiana do extrato bruto etanólico das folhas de Annona muricata L. (Annonaceae). Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota), 6(1), 26-30. http://dx.doi.org/10.18561/2179-5746/biotaamazonia. 6 (1), 20-25

Alves, E. G., Vinholis, A. H. C., Casemiro, L. A., Furtado, N. A. J. C., Silva, M. L. A., Cunha, W. R., & Martins, C. H. G. (2008). Estudo comparativo de técnicas de triagem para avaliação da atividade anti--bacteriana de extratos brutos de vegetais espécies e substâncias puras. Química Nova, 31 (5), 1224-1229. https://doi.org/10.1590/S0100-40422008000500052

Afroz, N., Hoq, A., Jahan, S., Islam, M., Ahmed, F., et al. (2019). Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities. Heliyon, 6(1), e03112. doi.org/10.1016/j.heliyon.2019.e03112

Brasil. Ministério da Saúde. Ministério da Saúde. (2016). Política e Programa Nacional de Plantas e Fitoterápicas. Ministério da Saúde

Brasil. Ministério da Saúde. Farmacopeia Brasileira. (2019). Agência Nacional de Vigilância Sanitária. Brasília - DF: Anvisa (6)2, https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/farmacopeia-brasileira/arquivos/7989json-file-1

Beneval, B., Monteiro, E. B., Lemos, Á. S., Junior, I. C. B., Oliveira, F. E., Menezes, D. R. A., Irwin, R., & Kerntopf, M. R. (2016). Estudo etnofarmacológico comparativo na região do Araripe da Annona muricata L. (Graviola). Revista Cubana de Plantas Medicinales, 21 (1), 9-19.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S102847962016000100002&lng=es&tlng=pt

Bylka, W., Matlawska, I., & Pilewski, N. A. (2004). Natural Flavonoids as Antimicrobial Agents. Mylan School of Pharmacy, 7(2), 24 – 31.

Clinical Laboratory Standards Institute (CLSI). (2019) Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement. NCCLS document M100-521, 34(1)

Dani, C., Aginoni, J. C., Calloni, C., Salvador, M., & Spada, P. D. S. (2010). Viabilidade celular de cultura de linfócitos tratados com Annona muricata L. Biociências e Saúde, 12(24), 95-101. http://dx.doi.org/10.15602/1983-9480/cmbs. 12 (24), 95-101.

Debalke, D., Birhan, M., Kinubeh, A., & Yayeh, M. (2018). Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts of Sida rhombifolia's Aerial Part. The Scientific World Journal, (Artigo ID 8429809), 1-8. https://doi.org/10.1155/2018/8429809

Dolab, J., Lima, B., Spaczynska, E., Kos, J., Cano, N., Feresin, G, Tapia, A, Garibotto, F, Petenatti, E, Olivella, M, Musiol, R, Jampilek, J., & Enriz, R (2018). A atividade antimicrobiana de Annona emarginata (Schltdl.) H. Rainer e os compostos isolados mais ativos contra bactérias clinicamente importantes. Moléculas, 23 (5), 1187. 10.3390 / moléculas23051187

Dutra, F. S. G., Carlos, L. A., Motta, O. V., Vianna, A. P., & Pereira, S. M. F. (2016). Atividade antimicrobiana de extratos vegetais frente à bactérias de importância médica. Biológicas & Saúde, 6(20). https://doi.org/10.25242/88686202016965

Filho, C. V., & Yunes, R. A. (1998). Estratégias para a obtenção de compostos farmacologicamente ativos a partir de plantas medicinais: conceitos sobre modificação estrutural para otimização da atividade. Química Nova, 21 (1), 99-105. https://doi.org/10.1590/S0100-40421998000100015

Galvão, S. S., Monteiro, A. S., Siqueira, E. P., Bomfim, M. R. Q., Souza, M. V. D., Ferreira, G. F., Denadai, A. M. L., Santos, A. R. C., Santos, V. L., Fagundes, E. M. S., Fernandes, E. S., & Neto, V. M. (2016). Annona glabra Flavonoids Act As Antimicrobials by Binding to Pseudomonas aeruginosa Cell Walls. Frontiers in microbiology, 7, 2053. https://doi.org/10.3389/fmicb.2016.02053

Grove, D. C., & Randall, W. A. (1955). Assay methods of antibiotics: A laboratory manual. (2a ed.). Medical Encyclopedia Inc.

Gonçalves, A. L., Filho, A. A., & Menezes, H. (2005). Estudo comparativo da atividade antimicrobiana de extratos de algumas árvores nativas. Arq. Inst. Biol. São Paulo, 72 (3), 353-358. www.biologico.sp.gov.br/docs/arq/V72_3/goncalves.PDF

Guimarães, D. O., Momesso, L. S., & Pupo, M. T. (2010). Antibióticos: importância terapêutica e perspectivas para a descoberta e desenvolvimento de novos agentes. Química Nova, 33(3), 667-679. https://doi.org/10.1590/S0100-40422010000300035

Haro, G, Utami, N. P., & Sitompul, E. (2014). Study of The Antibacterial Activities of Soursop (Annona muricata L.) Leaves. International Journal of Pharm Tech Research, (6)2, 575–581.

Haslam E. (1996). Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of natural products, 59(2), 205–215. https://doi.org/10.1021/np960040+

Li, Y, Luo, Y, Hu, Y, Zhu, D. D., Zhang, S, Liu, Z. J., Gong, H B., & Zhu, H. L. (2012). Design, synthesis and antimicrobial activities of nitroimidazole derivaties containing 1,3,4 – oxadiazole scaffold as FabH inhibitors. Bioorganic and Medicinal Chemistry, 20, 4316 – 4322.

Moghadamtousi, S. Z., Fadaeinasab, M., Nikzad, S., Mohan, G., Ali, H. M., e Kadir, H. A. (2015). Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities. International journal of molecular sciences, 16(7), 15625–15658. https://doi.org/10.3390/ijms160715625

Organização mundial da saúde. (2017). OMS publica lista de bactérias para quais se necessitam novos antibióticos urgentemente. https://www.paho.org/bra/index.php?option=com_content&view=article&id=5357:oms-publica-lista-de-bacterias-para-as-quais-se-necessitam-novos-antibioticos-urgentemente&Itemid= 812

Parekh J., & Chanda, S. V. (2007). In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol (31), 53-58. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.465.1644&rep=rep1&type=pdf.

Rodrigues, C. G., Ferreira, P. R. B., Oliveira, C. S. M., Júnior, R. R., Valério, H. M., Bramdi, I. V., & Oliveira, D. A. (2014) Antibacterial activity of taninis from Psidium guineense Sw. (Myrtaceae). Journal of Medicinal Plant Research, (8), 1-5. https://doi.org/10.5897/JMPR2014.5500

Sanlukhe, D. K., Chavan, J. K., & Kadam, S. S. (1990). Dietary tannins: Consequences and remedies. Boca Raton: CRC Press. 1- 310.

Santana, P., Andreza, R., Leite, V., Sousa, P., Alves, A., Tintino, S., Oliveira, C., Figueredo, F., Rocha, G., Rocha, M A., Pereira, B., Costa, R, Rodrigues, F, Leandro, L., & Aquino, P. (2016). Efeito antibacteriano e antifúngico de extratos etanólico, hexânico e metanólico a partir de folhas de kalanchoe pinnata (Lam.) pers (Malva Corama) contra cepas multi-resistentes a drogas. Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota), 6(1), 64-69. http://dx.doi.org/10.18561/2179-5746/biotaamazonia. 6(1), 64-69.

Santos, S. C., Ferreira, F. S., Rossi-Alva, J. C, & Fernandez, L. G. (2007). Atividade antimicrobiana in vitro do extrato de Abarema cochliocarpos (Gomes) Barneby & Grimes. Revista Brasileira de Farmacognosia, 17 (2), 215-219. https://dx.doi.org/10.1590/S0102-695X2007000200014

Sato, M., Tsuchiya, H., Miyazaki, T., Fujiwara, S., Yamaguchi, R., Kureshiro, H., & Linuma, M. (1996). Antibacterial activity of hydroxychalcone against methicilin – resistance Staphylococcus aureus. International Journal of Antimicrobial Agents, (6), 227 – 231. https://doi.org/10.1016/0924-8579(95)00065-8

Silva, M., & Aquino, S. (2018). Antimicrobial resistance: a review of the challenges in the search for new treatment alternatives. Revista de Epidemiologia e Controle de Infecção, 8(4), 472-482. https://doi.org/10.17058/reci.v8i4.11580

Silva et al. (2011). Avaliação da atividade antibacteriana de extratos de Annona muricata L. (Annonaceae). Revista de Biologia e Farmácia. (6) 2, 27-36.

Published

09/05/2021

How to Cite

BATISTA, A. K. R. .; CESAR, K. K. F. A. .; PAULA, L. R. .; SILVA, F. L. da; RAPOSO, H. L. O. . Antibacterial potential (in vitro) of methanolic extract from Annona muricata L. Research, Society and Development, [S. l.], v. 10, n. 5, p. e33510514950, 2021. DOI: 10.33448/rsd-v10i5.14950. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/14950. Acesso em: 29 jan. 2023.

Issue

Section

Health Sciences