Effectiveness of the blue led in the photoinactivation of Staphylococcus aureus and Staphylococcus epidermidis in vitro





Staphylococcus aureus; Staphylococcus epiermidis; Phototherapy; Blue led; Antimicrobial; Photoinactivation.


One possibility of treatment in Aesthetics for folliculitis is a blue LED, as it acts on microbial control. Studies describe that the blue LED, with 405-470nm wavelength, has a bactericidal effect when irradiated in certain bacteria such as Staphylococcus aureus. This study aimed to evaluate the 450 nm blue LED's efficacy as a modality in aesthetic treatments in the photoinactivation of the planktonic strains of S. aureus and S. epidermidis with power densities of 97, 110, 156, and 200 mW/cm2 in different energy densities. Bacterial suspensions of S. aureus (ATCC 25923) and S. epidermidis (ATCC 12228) were plated in 24-well plates and irradiated with other energy and power densities. After irradiation, each bacterial suspension was diluted in a phosphate buffer solution in a 96-well plate. Aliquots of 10 µL were collected from this dilution and streaked, in triplicate, in Brain Heart Infusion agar plates and incubated for 24h/37 °C. CFU counts were expressed in log10/mL and submitted to ANOVA and Tukey statistical tests. The energy and power densities used were insufficient to cause an antimicrobial effect on S. aureus or S. epidermidis planktonic cultures with a single light application.


Albuquerque, B. D., Santos, K. Y. K., Galan, karolline C. A., Silva, L. S. S. da, Coelho, T. A., Mariano, I. C. de S., & Talhati, F. (2019). Tratamento estético para foliculite em homens. Pesquisa e Ação, 5, 35–39.

Ashkenazi, H., Malik, Z., Harth, Y., & Nitzan, Y. (2003). Eradication of Propionibacterium acnes by its endogenic porphyrins after illumination with high intensity blue light. FEMS Immunology and Medical Microbiology, 35(1), 17–24. https://doi.org/10.1016/S0928-8244(02)00423-6

Barolet, D. (2008). Light-Emitting Diodes (LEDs) in Dermatology. Seminars in Cutaneous Medicine and Surgery, 27(4), 227–238. https://doi.org/10.1016/j.sder.2008.08.003

Brown, M. M., & Horswill, A. R. (2020). Staphylococcus epidermidis-Skin friend or foe? PLoS Pathogens, 16(11), 1–6. https://doi.org/10.1371/JOURNAL.PPAT.1009026

Bumah, V. V., Masson-Meyers, D. S., Cashin, S., & Enwemeka, C. S. (2015). Optimization of the Antimicrobial Effect of Blue Light on Methicillin-Resistant Staphylococcus aureus (MRSA) In Vitro Violet. Lasers in Surgery and Medicine, 47(3), 266–272. https://doi.org/10.1016/j.physbeh.2017.03.040

Byrd, A. L., Deming, C., Cassidy, S. K. B., Harrison, O. J., Ng, W. I., Conlan, S., & Kong, H. H. (2017). Staphylococcus aureus and Staphylococcus epidermidis strain diversity underlying pediatric atopic dermatitis. Science Translational Medicine, 9(397), 1–22. https://doi.org/10.1126/scitranslmed.aal4651

Claudel, J. P., Auffret, N., Leccia, M. T., Poli, F., Corvec, S., & Dréno, B. (2019). Staphylococcus epidermidis: A Potential New Player in the Physiopathology of Acne? Dermatology, 235(4), 287–294. https://doi.org/10.1159/000499858

Dai, T., Gupta, A., Huang, Y. Y., Yin, R., Murray, C. K., Vrahas, M. S., & Hamblin, M. R. (2013). Blue light rescues mice from potentially fatal pseudomonas aeruginosa burn infection: Efficacy, safety, and mechanism of action. Antimicrobial Agents and Chemotherapy, 57(3), 1238–1245. https://doi.org/10.1128/AAC.01652-12

Dai, T., Gupta, A., Murrayd, C. K., Vrahase, M. S., Tegosa, G. P., & Hamblin, M. R. (2012). Blue light for infectious diseases: Propionibacterium acnes, Helicobacter pylori, and beyond? Drug Resistance Updates, 15(4), 223–236. https://doi.org/10.1016/j.drup.2012.07.001.Blue

Enwemeka, C. S., Williams, D., Hollosi, S., Yens, D., & Enwemeka, S. K. (2008). Visible 405 nm SLD light photo-destroys methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Lasers in Surgery and Medicine, 40(10), 734–737. https://doi.org/10.1002/lsm.20724

Froes-Meyer, P., Morais Carreiro, E. de, Martiniano de Medeiros, N. B., Neves dos Santos Lindenmeyer, W. R., Varela Júnior, C. A., Valetim da Silva, R. M., … Galadari, H. (2018). Analysis of a protocol for the treatment of buttocks folliculitis. Journal of Dermatology & Cosmetology, 2(4), 185–189. https://doi.org/10.15406/jdc.2018.02.00074

Hamblin, M. R., & Hasan, T. (2004). Photodynamic Therapy: a new antimicrobial approach to infectious disease? Photochemical & Photobiological Sciences, 3(5), 436–450. https://doi.org/10.1039/b311900a.Photodynamic

Hon, K. L., Tsang, Y. C. K., Pong, N. H., Leung, T. F., & Ip, M. (2016). Exploring Staphylococcus epidermidis in atopic eczema: friend or foe? Clinical and Experimental Dermatology, 41(6), 659–663. https://doi.org/10.1111/ced.12866

Lipovsky, A., Nitzan, Y., Gedanken, A., & Lubart, R. (2010). Visible light-induced killing of bacteria as a function of wavelength: Implication for wound healing. Lasers in Surgery and Medicine, 42(6), 467–472. https://doi.org/10.1002/lsm.20948

Lister, J. L., & Horswill, A. R. (2014). Staphylococcus aureus biofilms: Recent developments in biofilm dispersal. Frontiers in Cellular and Infection Microbiology, 4(DEC), 1–9. https://doi.org/10.3389/fcimb.2014.00178

Maclean, M., MacGregor, S. J., Anderson, J. G., & Woolsey, G. (2008). High-intensity narrow-spectrum light inactivation and wavelength sensitivity of Staphylococcus aureus. FEMS Microbiology Letters, 285(2), 227–232. https://doi.org/10.1111/j.1574-6968.2008.01233.x

Maclean, M., MacGregor, S. J., Anderson, J. G., & Woolsey, G. (2009). Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array. Applied and Environmental Microbiology, 75(7), 1932–1937. https://doi.org/10.1128/AEM.01892-08

Monaco, M., Araujo, F. P. de, Cruciani, M., Coccia, E. M., & Pantosti, A. (2017). Worldwide Epidemiology and Antibiotic Resistance of Staphylococcus aureus Monica. Current Topics in Microbiology and Immunology (2017), 409, 21–56. https://doi.org/10.1007/82

Oyama, J., Fernandes Herculano Ramos-Milaré, Á. C., Lopes Lera-Nonose, D. S. S., Nesi-Reis, V., Galhardo Demarchi, I., Alessi Aristides, S. M., & Campana Lonardoni, M. V. (2020). Photodynamic therapy in wound healing in vivo: a systematic review. Photodiagnosis and Photodynamic Therapy, 30(January), 101682. https://doi.org/10.1016/j.pdpdt.2020.101682

Opländer, C., Hidding, S., Werners, F. B., Born, M., Pallua, N., & Suschek, C. V. (2011). Effects of blue light irradiation on human dermal fibroblasts. Journal of Photochemistry and Photobiology B: Biology, 103(2), 118–125. https://doi.org/10.1016/j.jphotobiol.2011.02.018

Papageorgiou, P., Katsambas, A., & Chu, A. C. (2000). Phototherapy with blue (415 nm) and red (660 nm) light in the treatment of acne vulgaris. British Journal of Dermatology, 142(5), 973–978. https://doi.org/10.1046/j.1365-2133.2000.03481.x

Ribeiro, M. S., & Zezell, D. M. (2004)."Laser de baixa intensidade." A Odontologia e o laser. São Paulo: Quintessense

Rupel, K., Zupin, L., Ottaviani, G., Bertani, I., Martinelli, V., Porrelli, D., & Zacchigna, S. (2019). Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress. Npj Biofilms and Microbiomes, 5(1), 1–11. https://doi.org/10.1038/s41522-019-0102-9

Santajit, S., & Indrawattana, N. (2016). Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens. BioMed Research International, 2016. https://doi.org/10.1155/2016/2475067

Tong, S. Y. C., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler, V. G. (2015). Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28(3), 603–661. https://doi.org/10.1128/CMR.00134-14

Travers, J. B., Kozman, A., Yao, Y., Ming, W., Yao, W., Turner, M. J., & Chandan Saha. (2012). Treatment outcomes of secondarily impetiginized pediatric atopic dermatitis lesions and the role of oral antibiotics. Pediatric Dermatology, 29(3), 289–296. https://doi.org/10.1038/jid.2014.371




How to Cite

PEDROSO, J. T. .; PONCE, E.; RIBEIRO, I. de P. .; PINTO, J. G. .; GUILLERMO MIÑÁN, A.; FERREIRA-STRIXINO, J. Effectiveness of the blue led in the photoinactivation of Staphylococcus aureus and Staphylococcus epidermidis in vitro. Research, Society and Development, [S. l.], v. 11, n. 2, p. e37511225630, 2022. DOI: 10.33448/rsd-v11i2.25630. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/25630. Acesso em: 29 feb. 2024.



Health Sciences