In vitro multiplication of Eugenia calycina Cambess. (Myrtaceae) under the influence of BAP and NAA

Authors

DOI:

https://doi.org/10.33448/rsd-v12i10.43380

Keywords:

Micropropagation; Growth regulators; Cytokinin; Auxin.

Abstract

Eugenia calycina Cambess. (Myrtaceae) is a fruit species native to Brazil indicated for the recovery of degraded areas. Generally its propagation occurs by seeds, but leads to an increase in the juvenile period of the plants, problems in maintaining seed viability and pathogen incidence. However, with the application of tissue culture techniques it is possible to produce seedlings in large scale, uniformity of the plants obtained and reduction in the period of seed germination. Thus, the objective of this research was to define the best medium for in vitro multiplication of E. calycina. Nodal segments of 1 cm, extracted from E. calycina plants already established in vitro, were used as explants. The treatments consisted of different concentrations of BAP (0; 0.5; 1 and 2 mg L-1) and NAA (0; 0.5; 1 and 2 mg L-1). The experimental design was completely randomized, in a 4x4 factorial scheme, with four concentrations of BAP and four concentrations of NAA, with four replications and three tubes per replication. After 90 days, shoot number, length and fresh mass of the aerial part, production and fresh mass of callus were evaluated. The MS medium plus 2.0 mg L-1 of BAP is ideal for in vitro multiplication of E. calycina.

References

Arruda, A. L., Buss, M., Nerbass, F. R., & Rufato, L. (2019). Concentrações de citocinina influenciam a multiplicação in vitro de kiwizeiro. Evidência, 19(1), 45-56. https://doi.org/10.18593/eba.v19i1.19723

Asmar, S. A., Resende, R. F., Araruna, E. C., Morais, T. P., & Luz, J. M. Q. (2011). Citocininas na multiplicação in vitro de hortelã-pimenta (Mentha x Piperita L.). Revista Brasileira de Plantas Medicinais, 13, 533-538. https://doi.org/10.1590/S1516-05722011000500005

Asmar, S. A., Resende, R. F., Araruna, E. C., Morais, T. P., & Luz, J. M. Q. (2012). Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.) NE Brown]. Revista Brasileira de Plantas Medicinais, 14, 149-153. https://doi.org/10.1590/S1516-05722011000500005

Bordignon, K., Nagel, J. C., Sommer, L. R., & Heinzmann, N. (2022). Disinfestation, in vitro propagation and subculture of Pereskia aculeata Mill. Research, Society and Development, 11(13), 1-10. https://10.33448/rsd-v11i13.35548

Carvalho, J. M. F. C., & Vidal, M. S. (2003). Noções de Cultivo de Tecidos Vegetais. Embrapa Algodão.

Ferreira, D. F. (2011). SISVAR: A computer statistical analysis system. Ciência e Agrotecnologia, 35(6), 1039-1042. https://doi.org/10.1590/S1413-70542011000600001

Fermino Junior, P. C. P., & Scherwinski-Pereira, J. E. (2012). Germinação e propagação in vitro de cerejeira (Amburana acreana (Ducke) AC Smith-Fabaceae). Ciência Florestal, 22(1), 1-9. https://doi.org/10.5902/198050985074

Melo, B. de, Pinto, J. E. B. P., Luz, J. M. Q., Peixoto, J. R. & Juliatti, F. C. (2002). Diferentes antioxidantes no controle da oxidação, germinação e desenvolvimento das plântulas na cultura in vitro de embriões da guarirobeira [Syagrus oleracea (Mart.) Becc.]. Ciência e Agrotecnologia, 21(6), 1301-1306.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nascimento, A. C., Paiva, R., Nogueira, R. C., Porto, J. M. P., Nogueira, G. F., & Soares, F. P. (2008). BAP e AIB no cultivo in vitro de Eugenia pyriformis Cambess. Revista Acadêmica: Ciências Agrárias e Ambientais, 6(2), 223-228.

Pinhal, H. F., Anastacio, M. R., Carneiro, P. A. P., Silva, V. J., Morais, T. P., & Luz, J. M. Q. (2011). Aplicações da cultura de tecidos vegetais em fruteiras do Cerrado. Ciência Rural, 41(7), 1136-1142. https://doi.org/10.1590/S0103-84782011005000089

Rodrigues, F. A., Penoni, E. S., Soares, J. D. R., & Pasqual, M. (2013). Diferentes concentrações de sais do meio MS e BAP na multiplicação in vitro de Physalis peruviana. Bioscience Journal, 29(1), 77-82.

Salem, J., Hassanein, A., El-Wakil, D. A., & Loutfy, N. (2022). Interaction between growth regulators controls in vitro shoot multiplication in Paulownia and selection of NaCl-tolerant variants. Plants, 11(498), 1-17. https://doi.org/10.3390/plants11040498

Silva, J. A. da, Silva, D. B. da, Junqueira, N., & Andrade, L. R. M. de. (1992). Coleta de sementes, produção de mudas e plantio de espécies frutíferas nativas dos cerrados: informações exploratórias.

Souza, F. X., Sousa, F. H. L., Freitas, J. B. S., & Rossetti, A. G. (2000). Aspectos morfológicos da unidade de dispersão de cajazeira. Pesquisa Agropecuária Brasileira, 35(1), 215-220. https://doi.org/10.1590/S0100-204X2000000100024

Souza, J. A., Schuch, M. W., Donini, L. P., & Ribeiro, M. D. F. (2008). Tipos e concentrações de citocinina na multiplicação in vitro de pitangueira. Ciência Rural, 38(7), 2046-2048. https://doi.org/10.1590/S0103-84782008000700040

Villa, F., Araújo, A. G. de., Pio, L. A. S., & Pasqual, M. (2005). Multiplicação in vitro da amoreira-preta 'Ébano' em diferentes concentrações de meio MS e BAP. Ciência Agrotecnologia, 29(3), 582-589. https://doi.org/10.1590/S1413-70542005000300011

Villa, F., Fráguas, C. B., Dutra, L. F., Pio, L. A. S., & Pasqual, M. (2006). Multiplicação in vitro de amoreira-preta cultivar Brazos. Ciência e Agrotecnologia, 30(2), 266-270. https://doi.org/10.1590/S1413-70542006000200011

Villa, F., Pasqual, M., Rodrigues, F. A., Souza, A. das G., & Rezende, J. C. de. (2009). Efeito do sulfato de adenina e de 6-benzillaminopurina no crescimento in vitro de porta-enxertos de videira. Ciência e Técnica Vitivinícola, 24(1), 41-46.

Von Bulow, J. F., Carmona, R., & Parente, T. V. (1994). Armazenamento e tratamento de sementes de pitanga-vermelha-do-cerrado (Eugenia calycina). Pesquisa Agropecuária Brasileira, 29(6), 961-970.

Downloads

Published

05/10/2023

How to Cite

RODRIGUES, F. A. .; RODRIGUES, V. A.; DÓRIA, J.; PASQUAL, M. In vitro multiplication of Eugenia calycina Cambess. (Myrtaceae) under the influence of BAP and NAA. Research, Society and Development, [S. l.], v. 12, n. 10, p. e40121043380, 2023. DOI: 10.33448/rsd-v12i10.43380. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/43380. Acesso em: 14 may. 2024.

Issue

Vol. 12 No. 10

Section

Agrarian and Biological Sciences

License

Copyright (c) 2023 Filipe Almendagna Rodrigues; Vantuil Antonio Rodrigues; Joyce Dória; Moacir Pasqual

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.