Research and development of alternative natural histological dyes from plant species from the Atlantic Forest
DOI:
https://doi.org/10.33448/rsd-v12i13.43953Keywords:
Histology; Dyes; Atlantic forest; Croton.Abstract
Histological staining is an essential technique for the cellular and tissue study of organs, which makes it possible to identify structural components with the help of dyes. Some staining techniques have gained dominance in laboratory routine, although many synthetic dyes used contain high toxicity, which leads to the growing search for alternative natural histological dyes. In this sense, the objective of this work is to evaluate the coloring potential of pigments obtained from nine plant species derived from and remaining from the Atlantic Forest in sections of animal and plant tissue. The results showed that six extracts promoted coloring of tissue components of the intestine, heart, brain and skin of rats. Collagen fibers were evidenced by Croton floribundus, Croton urucurana, Ocotea odorífera, Xylopia brasiliensis, and mucopolysaccharides by Croton floribundus and Croton urucurana. Vascular structures and cell walls in plant tissue sections were highlighted in Solidago microglossa staining. And finally, the possible potential of some species derived from the Atlantic Forest was proven and may contain important staining value and contribute to the development of alternative means of histological staining.
References
Al-Tikritti, S. A. & Walker, F. (1978). Anthocyanin BB: a nuclear stain substitute for haematoxylin. Journal of Clinical Pathology. 31(2): 194-6
Anterino, S., Paiva, T. M. N., Silva, P., Zoby, L. C., Ferreira, J. M. & Motta Sobrinho, M. A. (2014). Adsorção do corante Eosina a partir de solução aquosa utilizando casca de marisco Anomalocardia brasiliana. XX Congresso Brasileiro de Engenharia Química.
Arruda, A. M. V., Fernandes, R. T. V., Silva; J. M. & Lopes, D. C. (2008). Avaliação morfo-histológica da mucosa intestinal de coelhos alimentados com diferentes níveis e fontes de fibra. Revista Caatinga, Revista Caatinga, 21(2): 01-11.
Avwioro, O. G., Onwuuka, S, K., Moody, J. O., Agbedahusi, J. M., Oduola, T., Ekpo, O. E & Oladele, A. A. (2007). Curcuma longa extract as a histological dye for collagen fibres and red blood cells. Anatomical Society Britain and Ireland, 210, 600-3.
Barcat, J. A. 2003. Orceína y fibras elásticas. Medicina Buenos Aires, 63 (5): 453-6.
Bassey, R. B., Oremosu, A. A. & Osinubi, A. A. A. (2012). Curcuma longa: Staining Effect on Histomorphology of the Testis. Macedonian Journal of Medical Science, 1 (5): 26-9.
Carrapiço, F. J. N. (1998). Tecidos vegetais estrutura e enquadramento evolutivo. Departamento de biologia vegetal/ Secção de Biologia celular e biotecnologia vegetal. V unico
Candido, H. M. N., Pereira, A. L., Cortines, E., Azevedo, M. M. DE, (2018), Censo Florestal de um trecho de Mata Ciliar urbana do Rio Paraíba do Sul, Três Rios, Diversidade e Gestão, RJ., 2(1):17-25.
Cheng, C. W., Md Saad, S., Abdullah, R., (2014). Alternative staning using extract of hibiscus (Hibiscus rosa-sinesis L.) and red beet (Beta vulgaris) in diagnosing ova of intestinal nematodes (Teichuris trichiuria and Ascaris lumbricoides). Europe Journal of Biotechnology and Bioscience, 1(5): 14-8.
Chukwu, O. O. C., Odu, C. E., Chukwu, D. I., Hafiz, N., Chidzie, V. N. & Onyimba, I. A. (2011). Application of extracts of Henna (Lawsonia inamis) leaves as a counter stain, African Journal of Microbiology Research, 5(21): 3351-6.
Daryani, A., Sharif, M. & Meigouni, M. (2011). Staning of Facicola hepatica by natural herbal dyes, Comparative clinical pathology, 20(4): 305-8.
Egbujo, E. C., Adisa, O. J. & Yahaya, H. B. (2008). A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histology Section of the Testis, International Journal of Morpholgy, 26(4): 927-930.
Gartner, P. L. & Hiatt, L. J. (2007). Tratado de Histologia em cores, 3ed por Elsevier Editora Ltda.
Golunski, C. M., Miotto, S. P. S., Junior, C. V., Corazza, T., Mienczki-Pereira, A. A., Mossi, A. J., Budke, J. C. & Casian, R. L. (2015). Diversidade e estrutura genética em Ocotea odorifera (Vell) Rohwer (Lauraceae) no Sul do Brasil. PERSPECTIVA, Erechim. 39(145): 41-52.
Junior, I. W.; Trindade, M. R. M.; Cerski, C. T. (2003). O colágeno em fáscia transversal de pacientes com hérnia inguinal direta submetidos à videolaparoscopia, Acta Cirúrgica Brasileira - Vol 18 (3): 196-202.
Kodjikian, L., Richthr,T., Beby, F., Flueckiger,F., Boehnke, M. & Garweg, J. G. (2005). Springer Nature, Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium, National Library of Medicine, 243(9): 917–925. https://doi:10.1007/s00417-004-1121-6.
Kusculu, N. & Ester, F. (2022). Applicability of alkanet (Alkanna tinctoria) extract for the histological staining of liver tissue, Journal of the Indian Chemical Society, 4(99)1-5. https://doi.org/10.1016/j.jics.2022.100409.
Lopes, E. J. De S., Pereira, R. M., Mundim, F. G. L. & Mendonça, A. R. Dos A. (2019). Padronização do corante natural extraído à partir do urucum (Bixa orellana)e sua aplicação na histologia. Revista Eletrônica Acervo Saúde. 11 (4) 1-12. https://doi.org/10.25248/reas.e241.2019.
Lorenzi, H. & Souza, V. C. (2019). Botânica sistemática, (4a ed.), Instituto Plantarum de Estudos da Flora Ltda SP.
Mahaba, Z. & Haniloo, A. (2018). Staining of Parasitic Helminths by Extracts of Allium cepa, Juglans regia, and Rubia tinctorum: An Approach to Herbal Dyes., Iranian Journal of Parasitology. 13(2)293-300
Mittal, A., Jhare, D., Mittal, J. (2013). Adsorption of hazardous dye Eosin Yellow from aqueous solution onto waste material De-oiled Soya: Isotherm, kinetics and bulk removal. Journal of Molecular Liquids, (179) 133-144.
Moreira, I. C., Lago, J. H. G., Young, M. C. M. & Roque, N. F. 2003. Antifungal Aromadendrane Sesquiterpenoids from the Leaves of Xylopia brasiliensis, Sociedade Brasileira de Química, 14(5): 828-831.
Nunes, C. S. & Cinsa, L. A. (2016). Princípios do processamento histológico de rotina, Revista Interdisciplinar de Estudos Experimentais, 8(Único): 31-40.
Okolie, N. J. C. (2008). Staning of Ova of Intestinal Parasites with Extracts of Hibiscus sabdariffa and Azadirachta indica, International Science Research Journal, 1(2): 116-9.
Oliveira, M. A. B., Pereira, S. T., Mendonça, A. R. Dos A.; Lopes, E. J. De S.; Mundim, F. G. L.; Pereira, R. M. (2018). Extratos de morus nigra l. (amora-preta) e bixa orellana l. (urucum) para substituição dos corantes hematoxilina e eosina (HE) na técnica histológica de rotina. Revista Eletrônica Acervo Saúde. 11 (2) 1-10.
Rohde, D. C., Silveira, S. O. & Vargas, V. R. A. (2006). O uso do corante do urucum (Bixa orellana L.) na técnica de coloração histológica* Revista Brasileira de Análises Clínicas, 38(2): 119-121.
Sá, I. M.; Valle, L. S. & Almeida, G. S. (2007). A Tradição do Uso de Plantas Tintoriais da Comunidade Rural de Santo Antonio do Rio Grande, Revista Brasileira de Biociências, Porto Alegre, 5(1): 276-278.
Silva, P. M. Dos S., Faschitello, T. R., Queiroz, R. S. De, Freeman, H. S., Costa, S. A. Da, Leo, P., Mondemor, A. F., Costa, S. M. Da, Natural dye from Croton urucurana Baill. bark: Extraction, physicochemical characterization, textile dyeing and color fastness properties, Journal & Books Science Direct Elsevier, v173, https://doi.org/10.1016/j.dyepig.2019.107953.
Soldera, C. C.; Zanella, G. N. & Frasson, A. P. Z., (2010). Avaliação da atividade antimicrobiana da Croton urucurana. Revista contexto & saúde, Ijuí-MT, Editora Unijuí, 10(19) 25-31.
Souza, H.Q., Hidalgo, A. F., Chaves, F. C. M. (2007). Preparo do corante (Arrabidaea chica (Bonpl.) B, Verl.) e sua Aplicação em Histologia. Horticultura Brasileira, 25(1): 36.
Souza, L. M. (2011). Estudo fitoquimico de Macroptilium lathyroides (1) urb. (Fabaceae). Universidade Federal do Ceará – Programa de Pós-Graduação em Quimica III.
Timm, L. L. (2005). Técnicas rotineiras de preparação e análise de lâminas Histológicas, Caderno La Salle XI, Canoas, 2(1): 231-9.
Wiam, I. M., Sonfada, M. I., Oke, B. O., Kwari, H. D., & Onyeyili, P. A. (2006). Lawsonia inermis And Hibiscus sabdariffa: Posible Histological Stains. Tropical Veterinarian, 24(1-2), 1-5.
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