In vitro evaluation of the antimicrobial activity of the association of metabolites secreted from the symbiotics kefir and resveratrol, against microorganisms isolated from patients with hospital infections

Authors

DOI:

https://doi.org/10.33448/rsd-v12i12.43973

Keywords:

Probiotics; Prebiotics; Bacterial growth; Kefir; Resveratrol.

Abstract

Hospital-acquired infections are considered a public health problem, with an increasing impact on mortality and morbidity rates among patients and on the costs associated with hospital care. According to the World Health Organization (WHO), antimicrobial resistance is seen as one of the top ten global threats to public health, with its persistence mainly attributed to the inappropriate and excessive use of antimicrobials. In a review of the clinical development of antibiotics aimed at priority resistant pathogens, only six antibiotics were deemed promising by the WHO in 2019. Bacteria causing common infections are the group with the highest antibiotic resistance rates, underscoring the urgency for therapeutic alternatives. It is in this context that the search for new natural antimicrobial alternatives becomes advantageous. This study aims to evaluate, in vitro, the antimicrobial activity of the combination of metabolites secreted from the probiotics kefir and resveratrol against bacteria isolated from patients with hospital-acquired infections.

References

Abedini, E., Khodadadi, E., Zeinalzadeh, E., Moaddab, S. R., Asgharzadeh, M., Mehramouz, B., Dao, S., & Samadi Kafil, H. (2021). A Comprehensive Study on the Antimicrobial Properties of Resveratrol as an Alternative Therapy. Evidence-based complementary and alternative medicine : eCAM, 2021, 8866311. https://doi.org/10.1155/2021/8866311

Azizi, N. F., Kumar, M. R., Yeap, S. K., Abdullah, J. O., Khalid, M., Omar, A. R., Osman, M. A., Mortadza, S. A. S., & Alitheen, N. B. (2021). Kefir and Its Biological Activities. Foods (Basel, Switzerland), 10(6), 1210. https://doi.org/10.3390/foods10061210

Barros, L. M., Bento, J. N. C., Caetano, J. A., Moreira, R. A. N., Pereira, F. G. F., Frota, N. M., Araujo, T. M. de, & Soares, E. (2012). Prevalência de microorganismos e a sensibilidade antimicrobiana de infecções hospitalares em uma unidade de terapia intensiva de um hospital público. Revista de Ciências Farmacêuticas Básica e Aplicada, 33, 429-435. https://rcfba.fcfar.unesp.br/index.php/ojs/article/view/281/279

Bengoa, A. A., Iraporda, C., Garrote, G. L., & Abraham, A. G. (2019). Kefir micro-organisms: their role in grain assembly and health properties of fermented milk. Journal of applied microbiology, 126(3), 686–700. https://doi.org/10.1111/jam.14107

Berman, A.Y., Motechin, R.A., Wiesenfeld, M.Y., & et al. (2017). The therapeutic potential of resveratrol: a review of clinical trials. npj Precision Onc, 1, 35. https://doi.org/10.1038/s41698-017-0038-6

Carreon FT, Mora KA, Barrera ICR, Andrade A. (2022) Serratia marcescens antibiotic resistance mechanisms of an opportunistic pathogen: a literature review. PeerJ. http://doi.org/10.7717/peerj.14399

Clinical and Laboratory Standards Institute. (2023). Performance standards for antimicrobial susceptibility testing (33rd ed.). CLSI supplement M100.

Erdogan, F. S., Ozarslan, S., Seydim, Z. B. G., & Tas, T. K. (2019). The effect of kefir produced from natural kefir grains on the intestinal microbial populations and antioxidant capacities of Balb/c mice. Food Research International, 115, 408-413. https://doi.org/10.1016/j.foodres.2018.10.080

Erginkaya, Z., Turhan, E. U., & Tatlı, D. (2018). Determination of antibiotic resistance of lactic acid bacteria isolated from traditional Turkish fermented dairy products. Iranian journal of veterinary research, 19(1), 53–56.

Estrela, C. (2018). Metodologia Científica: Ciência, Ensino, Pesquisa. Editora Artes Médicas.

Marques, V. D., Franzolin, M. R., Sanabani, S. S., Vigerelli, H., Piazza, R. M. F., Pimenta, D. C., Venâncio, T., Neves, I. V., de Sousa Silva, H. G., Dos Santos Courrol, D., Mendonça-Previato, L., Previato, J. O., Jorge, S. A. C., & de Oliveira Domingos, M. (2020). A new class of antimicrobial molecules derived from kefir, effective against Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus (MRSA) strains. Scientific reports, 10(1), 17434. https://doi.org/10.1038/s41598-020-73651-7

Michelin, A.F., & Fonseca, M.R. (2018). Perfil epidemiológico das infecções hospitalares na unidade de terapia intensiva de um hospital terciário.

Monika, Sharma, S., & Kumar, N. (2020). Use of plant derived antimicrobials as an alternative to antibiotics. J Pharmacogn Phytochem, 9(2), 1524-1532. https://doi.org/10.22271/phyto.2020.v9.i2y.11069

Novelle, M. G., Wahl, D., Diéguez, C., Bernier, M., & de Cabo, R. (2015). Resveratrol supplementation: Where are we now and where should we go? Ageing Research Reviews, 21, 1-15. https://doi.org/10.1016/j.arr.2015.01.002

Rosa, D. D., Dias, M. M. S., Grześkowiak, Ł. M., Reis, S. A., Conceição, L. L., & Peluzio, M. D. C. G. (2017). Milk kefir: nutritional, microbiological and health benefits. Nutrition research reviews, 30(1), 82–96. https://doi.org/10.1017/S0954422416000275

Salehi, B., Mishra, A. P., Nigam, M., Sener, B., Kilic, M., Sharifi-Rad, M., Fokou, P. V. T., Martins, N., & Sharifi-Rad, J. (2018). Resveratrol: A Double-Edged Sword in Health Benefits. Biomedicines, 6(3), 91. https://doi.org/10.3390/biomedicines6030091

Santos, N. de Q. (2004). A resistência bacteriana no contexto da infecção hospitalar. Texto & Contexto Enfermagem, 13, 64–70. https://doi.org/10.1590/S0104-07072004000500007

Singh, A. P., Singh, R., Verma, S. S., Rai, V., Kaschula, C. H., Maiti, P., & Gupta, S. C. (2019). Health benefits of resveratrol: Evidence from clinical studies. Medicinal Research Reviews, 39(5), 1851-1891. https://doi.org/10.1002/med.21565

Turrini, R. N. T. (2002). Infecção hospitalar e mortalidade / Nosocomial infection and mortality. Revista da Escola de Enfermagem da USP, 36(2), 177-183. https://doi.org/10.1590/S0080-62342002000200011

Vestergaard, M., & Ingmer, H. (2019). Antibacterial and antifungal properties of resveratrol. International journal of antimicrobial agents, 53(6), 716–723. https://doi.org/10.1016/j.ijantimicag.2019.02.015

World Health Organization. Regional Office for Europe. (2022). WHO Country Office in Ukraine, 2020–2021 report. World Health Organization. Regional Office for Europe. https://apps.who.int/iris/handle/10665/363750

Zanirati, D. F., Abatemarco, M., Jr, Sandes, S. H. C., Nicoli, J. R., Nunes, Á. C., & Neumann, E. (2015). Selection of lactic acid bacteria from Brazilian kefir grains for potential use as starter or probiotic cultures. Anaerobe, 32, 70–76. https://doi.org/10.1016/j.anaerobe.2014.12.007

Published

14/11/2023

How to Cite

CARNEIRO, S. V. .; BRAGA, F.; ANDRADE, M. C. . In vitro evaluation of the antimicrobial activity of the association of metabolites secreted from the symbiotics kefir and resveratrol, against microorganisms isolated from patients with hospital infections . Research, Society and Development, [S. l.], v. 12, n. 12, p. e67121243973, 2023. DOI: 10.33448/rsd-v12i12.43973. Disponível em: https://www.rsdjournal.org/index.php/rsd/article/view/43973. Acesso em: 26 feb. 2024.

Issue

Section

Health Sciences