Cytotoxicity and residual effect of ethanol extract of pequi peel on canine osteosarcoma cells




Dog; Chemotherapeutic; Survival; Tumor; Viability.


The development of new chemotherapy drugs is a premise for the scientific community in recent years, mainly through the use of plants and other natural sources. In this context, analyses of cytotoxicity and residual effect have become essential for the establishment of new therapies. Therefore, this study aims to evaluate the cytotoxic and residual effects of ethanol extract of pequi peel (EEPP) on canine osteosarcoma cells. For this, we used analyses of cell viability, cytotoxicity, IC50, and cell survival after therapy in treatments of 24, 48, and 72 hours. We used the design of the randomized block, using an Analysis of Variance with Tukey’s posthoc test to compare the treatments, considering a 5% significance. The extract showed no cytotoxic effect within 24 hours of treatment, but a protective effect, or possibly a mitotic-inducing effect on osteosarcoma cells. However, at 48 and 72 hours, we found gradual cytotoxicity favoring the effective control of neoplastic cells, obtaining, on average, 55% of cytotoxicities at concentrations of 10 to 100 µg/mL. IC50 values were 4.05 µg/mL for the 48-hour group and 4.53 µg/mL for the 72-hour group. The treatment residual effect reduced cell growth after the exposure to the extract. The survival factor was lower (18.81%) in the 72-hour group, with a concentration of 10 µg/mL and higher (81.33%) in the 48-hour group, with a concentration of 100 µg/mL. These results suggest that the ethanol extract of pequi peel promotes the increase in cell viability within 24 hours, cytotoxic action in exposures over 48 hours, and time-dependent residual effect on canine osteosarcoma cells.


Arnhold E., (2013). Package in the R environment for analysis of variance and complementary analyses. Brazilian Journal of Veterinary Research and Animal Science. 50(6):488-492.

Brown H. K., Schiavone K., Gouin F., Heymann M. F., & Heymann D. (2018). Biology of bone sarcomas and new therapeutic developments. Calcified tissue international. 102(2):174-195.

Cruz V. S., Rodrigues F. A., Braga K., Machado P. A., Bianchi Filho C., Prado Y. C., & Araújo EG. (2018). β Lapachone blocks the cell cycle and induces apoptosis in canine osteosarcoma cells. Pesquisa Veterinária Brasileira. 38(12):2224-2232.

de Oliveira F. F. B., de Araújo J. C. B., Pereira A. F., Brito G. A. C., Gondim D. V., de Albuquerque Ribeiro R., de Menezes I. R. A., & Vale M. L. (2015). Antinociceptive and anti-inflammatory effects of Caryocar coriaceum Wittm fruit pulp fixed ethyl acetate extract on zymosan-induced arthritis in rats. Journal of ethnopharmacology. 174:452-463.

Evola F. R., Costarella L., Pavone V., Caff G., Cannavò L., Sessa A., Avondo S., & Sessa G. (2017). Biomarkers of osteosarcoma, chondrosarcoma, and Ewing sarcoma. Frontiers in pharmacology. 8:150.

Ferreira C. M. (2019). Análise química de extratos de Caryocar brasiliense com potencial antioxidante. Repository UFG. 2019:1-133.

Gabriel G. H., Nepomuceno L. L., Pimenta V. S., & de Araújo E. (2017). Agentes antineoplásicos para o tratamento do osteossarcoma. Cent Cient Conhecer-Goiânia. 14(26):152-66.

Miranda-Vilela A. L., Grisolia C. K., Longo J. P. F., Peixoto R. C. A., Almeida M. C., Barbosa L. C. P., Roll M. M., Portilho F. A., Estevanato L. L.C., Bocca A. L., Báo S. N., & Lacava Z. G. M., (2014). Oil rich in carotenoids instead of vitamins C and E as a better option to reduce doxorubicin-induced damage to normal cells of Ehrlich tumor-bearing mice: hematological, toxicological and histopathological evaluations. The Journal of nutritional biochemistry. 25(11):1161-1176.

Ozi J. M., Suffredini I. B., Paciencia M., Frana S. A., & Dib L. L. (2011). In vitro cytotoxic effects of Brazilian plant extracts on squamous cell carcinoma of the oral cavity. Brazilian oral research. 25(6):519-525.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica.[e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio. ufsm. br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica. pdf.

Plumb J. A. (2004). Cell sensitivity assays: clonogenic assay. Cancer cell culture: Springer. 159-164.

Roesler R., Catharino R. R., Malta L. G., Eberlin M. N., & Pastore G. (2008). Antioxidant activity of Caryocar brasiliense (pequi) and characterization of components by electrospray ionization mass spectrometry. Food Chemistry. 110(3):711-717.

Silva N. C. (2018). Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro. Repository UFG. 2019:1-52.

Souza M. R., de Carvalho R. K., de Carvalho L. S., de Sá S., Andersen M. L., de Araújo E. G., & Mazaro-Costa R. (2019). Effects of subchronic exposure to Caryocar brasiliense peel ethanolic extract on male reproductive functions in Swiss mice. Reproductive Toxicology. 87:118-124.

Stokol T., Wan C., Blakely R., Bellat V., & Law B. (2020). Aldoxorubicin-loaded nanofibers are cytotoxic for canine mammary carcinoma and osteosarcoma cell lines in vitro: A short communication. Research in veterinary science. 128:86-89.

Suárez A., Soto C., Gómez L., Gamboa Ó., Soto D., Escandón S., & Terselich G. (2017). Resultados del tratamiento de osteosarcoma convencional de alto grado en niños y adolescentes: análisis de supervivencia de una cohorte tratada sin metotrexato. revista colombiana de cancerología. 21(2):86-94.

Team R. C. (2013). R: A language and environment for statistical computing. Version 2.6.2.

Xie D., Zheng G. Z, Xie P., Zhang Q. H., Lin F. X., Chang B., Hu Q. X., Du S. X., & Li X. D. (2017). Antitumor activity of resveratrol against human osteosarcoma cells: a key role of Cx43 and Wnt/β-catenin signaling pathway. Oncotarget. 8(67):111419.




How to Cite

NEPOMUCENO, L. L. .; FERREIRA, C. M. .; SOARES, N. P. .; BORGES, J. C. de A. .; PEREIRA, D. K. S.; PEREIRA, K. F.; CRUZ, V. de S. .; FERREIRA, J. L. .; ARNHOLD, E. .; ARAÚJO, E. G. de . Cytotoxicity and residual effect of ethanol extract of pequi peel on canine osteosarcoma cells. Research, Society and Development, [S. l.], v. 9, n. 10, p. e8779109230, 2020. DOI: 10.33448/rsd-v9i10.9230. Disponível em: Acesso em: 8 jun. 2023.



Agrarian and Biological Sciences