Rumen fermentation, nitrogen balance and microbial responses to DDGS supplementation in sheep fed low-quality forages
DOI:
https://doi.org/10.33448/rsd-v14i10.49871Keywords:
Ruminants, Agro-industrial by-products, Protein concentrate.Abstract
The objective of this study was to evaluate the fermentative parameters and rumen microbial population of sheep fed with low quality forage (rhodes grass hay; Chloris gayana, Kunth) supplemented or not with distiller's dried grains with solubles (DDGS). Eight adult sheep (Ovis aries) were used and distributed into two treatments according to the diet received: 1) Hay (H), Rhodes grass hay alone, and 2) H + DDGS, Rhodes grass hay + DDGS (proportion 64:36 on a dry basis), both diets were offered ad libitum. During the experimental stage, feed intake was recorded, and rumen fluid samples were collected to analyze pH, volatile fatty acids (VFA) and rumen ammonia nitrogen (N-NH3). Animals supplemented with DDGS showed higher N-NH3 concentrations (P = 0.0007), while no significant differences were observed in VFA concentrations (P = 0.466) or rumen pH (P = 0.809) compared to those fed only rhodes grass hay. Animals supplemented with DDGS had higher concentrations of methanogenic archaea (P = 0.017) and sulfate-reducing bacteria (P > 0.009) in the rumen but did not present differences in the concentration of total bacteria (P=0.061). Although supplementation with DDGS promoted an improvement in nitrogen balance, no significant differences were detected between treatments (P = 0.094). In conclusion, animals supplemented with DDGS showed improvements in rumen fermentative activity without changes in pH and VFA compared to animals that received hay alone.
References
Association of Official Analytical Chemists International - AOAC. (1990). Official Methods of Analysis, 15th edn (edited by. K. Helrich) Pp. 1028–1039. Arlington, VA: Association of Official Analytical Chemists Inc.
Association of Official Analytical Chemists International – AOAC. (2005). Official methods of the Association of Official Analytical Chemists, 16th edn. Arlington, TX: Association of Official Analytical Chemists.
Balbuena, O., Slanac, A. L., Kucseva, D., & Manghera, P. M. (2008). Consumo de Gramma Rhodes con distintos niveles de suplementación con germen de maíz (Beca de Pregrado). Universidad Nacional del Nordeste, Facultad de Ciencias Veterinarias, 1-13.
Callaway, T. R., Dowd, S. E., Edrington, T. S., Anderson, R. C., Krueger, N., Bauer, N., ... & Nisbet, D. J. (2010). Evaluation of bacterial diversity in the rumen and feces of cattle fed different levels of dried distillers grains plus solubles using bacterial tag-encoded FLX amplicon pyrosequencing. Journal of Animal Science, 88(12), 3977-3983.
Castillo‐Lopez, E., Jenkins, C. J. R., Aluthge, N. D., Tom, W., Kononoff, P. J., & Fernando, S. C. (2017). The effect of regular or reduced‐fat distillers grains with solubles on rumen methanogenesis and the rumen bacterial community. Journal of Applied Microbiology, 123(6), 1381-1395.
Denman, S. E., Tomkins, N. W., & McSweeney, C. S. (2007). Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Mcrobiology Ecology, 62(3), 313-322.
Denman, S. E., & McSweeney, C. S. (2006). Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology, 58(3), 572-582.
Dryden, G. M. (2008). Animal nutrition science. Cabi.
Fernández Pepi, M.G., Wawrzkiewicz, M., Cerón Cucchi, M.E. y Alvarez Ugarte, D. (2019). Efecto de la incorporación de la burlanda de maíz en la dieta sobre el ambiente ruminal de ovinos. Revista Argentina de Producción Animal, NA 9 vol 39 supl. 1: 35.
Friggens, N. C., Oldham, J. D., Dewhurst, R. J., & Horgan, G. (1998). Proportions of volatile fatty acids in relation to the chemical composition of feeds based on grass silage. Journal of Dairy Science, 81(5), 1331-1344.
Fron, M., Madeira, H., Richards, C., & Morrison, M. (1996). The impact of feeding condensed distillers byproducts on rumen microbiology and metabolism. Animal Feed Science and Technology, 61(1-4), 235-245.
Gere, J. I., Feksa Frasson, M., Wawrzkiewicz, M., Fernández Pepi, M. G., Ramos, M. L., Bualó, R., ... & Jaurena, G. (2022). Enteric methane emission from Sheep fed with rhodes grass hay (Chloris gayana) alone or supplemented with dried distillers’ grains with solubles. Methane, 1(3), 210-217.
Goering, H. K., & Van Soest, P. J. (1970). Forage fiber analyses (apparatus, reagents, procedures, and some applications) (No. 379). US Agricultural Research Service.
Gouws, L., & Kistner, A. (1965). Bacteria of the ovine rumen IV. Effect of change of diet on the predominant type of cellulose-digesting bacteria. The Journal of Agricultural Science, 64(1), 51-57.
Hernández, O., López, A., García, E. M., Nazareno, M. A., & Arroquy, J. I. (2021). Dried distiller´ s grains plus solubles supplementation improves low-quality tropical grass utilization on beef steers. Ciência Rural, 52.
Hoffmann, A., Cardoso, A. S., Fonseca, N. V. B., Romanzini, E. P., Siniscalchi, D., Berndt, A., ... & Reis, R. A. (2021). Effects of supplementation with corn distillers’ dried grains on animal performance, nitrogen balance, and enteric CH4 emissions of young Nellore bulls fed a high-tropical forage diet. Animal, 15(3), 100155.
Hoover, W. H., & Stokes, S. R. (1991). Balancing carbohydrates and proteins for optimum rumen microbial yield. Journal of Dairy Science, 74(10), 3630-3644.
Hünerberg, M., McGinn, S. M., Beauchemin, K. A., Okine, E. K., Harstad, O. M., & McAllister, T. A. (2013a). Effect of dried distillers grains plus solubles on enteric methane emissions and nitrogen excretion from growing beef cattle. Journal of Animal Science, 91(6), 2846-2857.
Hünerberg, M., McGinn, S. M., Beauchemin, K. A., Okine, E. K., Harstad, O. M., & McAllister, T. A. (2013b). Effect of dried distillers’ grains with solubles on enteric methane emissions and nitrogen excretion from finishing beef cattle. Canadian Journal of Animal Science, 93(3), 373-385.
Jaurena, G., Wawrzkiewicz, M. (2018) PROMEFA (Programa para el mejoramiento de forrajes y alimentos). Guía de Protocolos Objetivos Estandarizados. Centro de Investigación y Servicios en Nutrición Animal, Buenos Aires - Argentina.
Johnson, K., Huyler, M., Westberg, H., Lamb, B., & Zimmerman, P. (1994). Measurement of methane emissions from ruminant livestock using a sulfur hexafluoride tracer technique. Environmental Science & Technology, 28(2), 359-362.
Leupp, J. L., Lardy, G. P., Karges, K. K., Gibson, M. L., & Caton, J. S. (2009). Effects of increasing levels of corn distillers dried grains with solubles to steers offered moderate-quality forage. Journal of Animal Science, 87(12), 4064-4072.
Li, Y., He, M., Li, C., Forster, R., Beauchemin, K. A., & Yang, W. (2012). Effects of wheat dried distillers' grains with solubles and cinnamaldehyde on in vitro fermentation and protein degradation using the Rusitec technique. Archives of Animal Nutrition, 66(2), 131-148.
Loy, T. W., MacDonald, J. C., Klopfenstein, T. J., & Erickson, G. E. (2007). Effect of distillers grains or corn supplementation frequency on forage intake and digestibility. Journal of Animal Science, 85(10), 2625-2630.
Martínez-Pérez, M. F., Calderón-Mendoza, D., Islas, A., Encinias, A. M., Loya-Olguín, F., & Soto-Navarro, S. A. (2013). Effect of corn dry distiller grains plus solubles supplementation level on performance and digestion characteristics of steers grazing native range during forage growing season. Journal of Animal Science, 91(3), 1350-1361.
Mehrez, A. Z., & Orskov, E. R. (1976). Rates of rumen fermentation in relation to ammonia concentration. The Proceedings of the Nutrition Society, 35(1), 40A-41A.
NASEM (National Academies of Sciences, Engineering, and Medicine). Nutrient requirements of beef cattle. (2016). 8th rev. ed. Washington (DC): National Academic Press.
NASEM (National Academies of Sciences, Engineering, and Medicine). Nutrient Requirements of Dairy Cattle. (2021). 8th rev. ed. Natl. Acad. Press, Washington, D.C.
Nocek, J. E., & Russell, J. (1988). Protein and energy as an integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. Journal of Dairy Science, 71(8), 2070-2107.
NRC - National Research Council. Nutrient requirements for small ruminants. 1st ed. Washington, D. C.: National Academy of Science, 2007.
NRC - National Research Council. (1985). Nutrient requirements of sheep (Vol. 6). National Academies Press.
Ortiz-Chura, A., Pepi, M. F., Wawrzkiewicz, M., Cucchi, M. C., Cravero, S., & Jaurena, G. (2018). Microbial populations and ruminal fermentation of sheep and llamas fed low quality forages. Small Ruminant Research, 168, 47-51.
Ørskov, E. R., and M. Ryle. 1990. Energy Nutrition in Ruminants. Elsevier Science Publishers, New York, NY.
Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. [free ebook]. Santa Maria: Editora da UFSM.
Russell, J. B. (1984). Factors influencing competition and composition of the rumen bacterial flora. Herbivore nutrition in the subtropics and tropics/edited by FMC Gilchrist and RI Mackie.
Sacido, M.; & Cicetti, G. (2016). Evaluation of the production, quality of three cultivars of rhodes grass (Chloris Gayana) and viability of seeds, introduced in the pampas. Journal of Environmental Science and Engineering, A7, (2016) 613-624.
Satter, L. D., & Roffler, R. E. (1975). Nitrogen requirement and utilization in dairy cattle. Journal of Dairy Science, 58(8), 1219-1237.
Satter, L. D., & Slyter, L. L. (1974). Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition, 32(2), 199-208.
Shitsuka, R. et al. (2014). Matemática fundamental para tecnologia. (2ed). Editora Érica.
Spence, C., Whitehead, T. R., & Cotta, M. A. (2008). Development and comparison of SYBR Green quantitative real‐time PCR assays for detection and enumeration of sulfate‐reducing bacteria in stored swine manure. Journal of Applied Microbiology, 105(6), 2143-2152.
Stern M.S., Calsamiglia S., Endres M.I., (1994). Dinámica del metabolismo de los hidratos de carbono y del nitrógeno en el rumen. En: X Curso de Especialización FEONA, Madrid.pp177-194.
Tebot, I., Ibarra, A. L., Purtcher, F., & Cirio, A. (2004). Influence of energy supply on microbial protein synthesis and renal urea handling in Corriedale sheep. Journal of Animal and Feed Sciences, 13, 223-226.
Vieira, S. (2021). Introdução à bioestatística. Editora GEN/Guanabara Koogan.
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Copyright (c) 2025 Monica Feksa Frasson, Marisa Wawrzkiewicz, Maria Gabriela Fernández Pepi, Jose Gere, Ricardo Bualó, Maria Esperanza Cerón-Cucchi, Gustavo Jaurena
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