Salivary proteomic analysis for the early detection of smoking-related lesions: A narrative literature review

Authors

DOI:

https://doi.org/10.33448/rsd-v14i12.50355

Keywords:

Proteomics, Saliva, Smoking.

Abstract

Smoking is considered a health hazard around the world, with high mortality rates and, above all, harmful consequences that manifest themselves years later. With the potential advances in the use of saliva, as it is a non-invasive, safe, and cheaper collection method for identifying the diagnosis of lesions caused by smoking through proteomic biomarkers, it is being considered a great attractive and useful tool for tests aimed at early results and reflecting the health of the human body. The article presents a qualitative literature review, with little structure and of the specific type of narrative literature review, addressing smoking and saliva, with the aim of detailing the descriptions of each marker that uses the human saliva proteome, showing with data the evidence of their effectiveness and results, also focusing on describing their advantages for use, in addition to discussing some challenges of research in this field.

References

Al-Amrani, S. et al. (2021). Proteomics: Concepts and Applications in Human Medicine. World Journal of Biological Chemistry. 12(5), 57–69.

Alhajj, M. & Babos, M. (2023). Physiology, salivation. Bethesda, MD: National Library of Medicine (US) c2024. https://pubmed.ncbi.nlm.nih.gov/31194408/.

Alharbi, R. A. (2020). Proteomics approach and techniques in identification of reliable biomarkers for diseases. Saudi Journal of Biological Sciences. 27(3), 968–74.

Bansal, P. V. et al. (2024). Assessing the diagnostic accuracy of salivary biomarkers in detecting oral squamous cell carcinoma. Journal of Pharmacy & Bioallied Sciences. 16(supl. 3), S2194–S2196.

Barros, O. et al. (2024). Shaping the future of oral cancer diagnosis: advances in salivary proteomics. Expert Review of Proteomics. Expert Rev Proteomics. 21(4):149-68. doi: 10.1080/14789450.2024.2343585.

Birhanu, A. G. (2023). Mass spectrometry-based proteomics as an emerging tool in clinical laboratories. atual. Bethesda, MD: National Library of Medicine (US), c2023. https://pmc.ncbi.nlm.nih.gov/articles/PMC10464495/.

Cotias, A. S. et al. (2022). O impacto do tabagismo na saúde humana: uma revisão de literatura. Revista Brasileira de Educação, Saúde e Bem-estar. 1(4).

Cui, M. et al. (2022). High-throughput proteomics: a methodological mini-review. Laboratory Investigation. 102(11), 1170–81.

Davidson, S. P. et al. (2023). Assessment of brush biopsy findings and salivary LDH levels in oral mucosal lesions of tobacco users. Indian Journal of Dental Research. 34(2), 191.

Morais, E. A. H. et al. (2022). Fatores individuais e contextuais associados ao tabagismo em adultos jovens brasileiros. Ciência & Saúde Coletiva. 27, 2349–62.

Dongiovanni, P. et al. (2023). Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation. International Journal of Oral Science. 15, 27.

Grocholska, P. et al. (2023). Qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics. Metabolites, Basel, Switzerland. 13(2), 155. DOI: https://doi.org/10.3390/metabo13020155.

Hu, H. & Leung, W. K. (2023). Mass spectrometry-based proteomics for discovering salivary biomarkers in periodontitis: a systematic review. International Journal of Molecular Sciences. 24(19), 14599.

Huang, Y. et al. (2023). Potential noninvasive biomarkers for the malignant transformation of oral leukoplakia: a systematic review and meta-analysis. Cancer Medicine. 12(13), 14718–30.

INCA. (2022a). Mortalidade no Brasil. Rio de janeiro: Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA). https://www.gov.br/inca/pt-br/assuntos/gestor-e-profissional-de-saude/observatorio-dapolitica-nacional-de-controle-do-tabaco/dados-e-numeros-do-tabagismo/mortalidade-nobrasil.

INCA. (2002b). Prevalência do tabagismo. Rio de Janeiro: Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA). https://www.gov.br/inca/pt-br/assuntos/gestor-e-profissional-de-saude/observatorio-dapolitica-nacional-de-controle-do-tabaco/dados-e-numeros-do-tabagismo/prevalencia-dotabagismo.

Leite, R. B. et al. (2021). A influência da associação de tabaco e álcool no câncer bucal: revisão de literatura. Jornal Brasileiro de Patologia e Medicina Laboratorial. 57, e2142021.

Lima, M. P. et al. (2020). Biomarcadores salivares no diagnóstico e monitoramento de patologias orais e sistêmicas. Revista Cubana de Estomatología. 57(1), 1–15.

Li, Y. & Hecht, S. S. (2022). Carcinogenic components of tobacco and tobacco smoke: a 2022 update. Food and Chemical Toxicology. 165, 113179.

Luo, J. et al. (2022). Cigarette smoking and risk of different pathologic types of stroke: a systematic review and dose-response meta-analysis. Frontiers in Neurology. 12, 25 jan. 2022.

Nonaka, T. & Wong, D. T. W. (2022). Saliva diagnostics. Annual Review of Analytical Chemistry. 15(1), 107–21.

Nunes, S. F. & Kock, K. S. (2020). Prevalência de tabagismo e morbimortalidade por câncer de pulmão nos estados brasileiros. Revista Brasileira de Medicina de Família e Comunidade. 19(46), 3598.

Pappa, E. et al. (2020). Proteomic advances in salivary diagnostics. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics. 1868(11), 140494, Amsterdam: Netherlands.

Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. [free ebook]. Santa Maria. Editora da UFSM.

Prakash, A. et al. (2020). Detection of salivary alkaline phosphatase levels in smokers, diabetic patients, potentially malignant diseases and oral malignant tumours. Journal of Pharmacy and Bioallied Sciences. 12(5), 430.

Rodríguez, L. M. et al. (2020). Salivary biomarkers and their application in the diagnosis and monitoring of the most common oral pathologies. International Journal of Molecular Sciences. 21(14), 5173.

Rother, E. T. (2007). Revisão sistemática x revisão narrativa. Acta Paulista de Enfermagem. 20(2), 5-6.

Roy, B. et al. (2024). Early diagnosis of oral submucous fibrosis using salivary 8-OHDG and 8- isoprostane. atual. Bethesda, MD: National Library of Medicine (US), c2024. https://pmc.ncbi.nlm.nih.gov/articles/PMC11795467/.

Sánchez, M. A. A. et al. (2025). Levels of IL-1β, MMP-8, and MMP-9 in the saliva of subjects with periodontitis: a systematic review and meta-analysis. Journal of Clinical Laboratory Analysis. 39(10), e70040.

Shreya, S. et al. (2023). Utility of salivary biomarkers for diagnosis and monitoring the prognosis of nicotine addiction: a systematic review. Journal of Oral Biology and Craniofacial Research. 13(6), 740–50.

Silva, J. R. T. C. et al. (2022). O tabagismo como fator de risco para o câncer bucal: principais evidências e tendências. Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde. 25(5-esp), 724-8.

Silva, P. G., Tavares, B. F. & De Souza, L. C. (2025). Proteômica aplicada ao diagnóstico precoce de doenças dermatológicas. Journal Archives of Health. 6(4), e2799.

Song, M. et al. (2023). Promising applications of human-derived saliva biomarker testing in clinical diagnostics. International Journal of Oral Science. 15(1).

Surdu, A. et al. (2025). Saliva as a diagnostic tool for systemic diseases: a narrative review. 61(2), 243. Basileia, Switzerland: Multidisciplinary Digital Publishing Institute (MDPI), c2025. https://www.mdpi.com/1648-9144/61/2/243.

Swaathi, R., Narayan, M. & Krishnan, R. (2024). Salivary biomarkers in cancer: scoping review. Oral Oncology Reports. 10, 100503.

Verma, A. et al. (2021). Comparative evaluation of salivary biomarker levels in e-cigarette smokers and conventional smokers. Journal of Pharmacy and Bioallied Sciences. 13(6), 1642.

WHO. (2020). Global report on trends in prevalence of tobacco use third edition. Geneva: World Health Organization (WHO). https://iris.who.int/server/api/core/bitstreams/2eca3aea-b772-4272-a2ae6fa26f3f9cd5/content.

Zięba, S. et al. (2024). Impact of smoking on salivary lipid profile and oxidative stress in young adults. atual. Bethesda, MD: National Library of Medicine (US), c2024. https://pmc.ncbi.nlm.nih.gov/articles/PMC10802080/.

Published

2025-12-18

Issue

Section

Health Sciences

How to Cite

Salivary proteomic analysis for the early detection of smoking-related lesions: A narrative literature review. Research, Society and Development, [S. l.], v. 14, n. 12, p. e134141250355, 2025. DOI: 10.33448/rsd-v14i12.50355. Disponível em: https://www.rsdjournal.org/rsd/article/view/50355. Acesso em: 2 jan. 2026.